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- W4285258416 abstract "Quartz crystal microbalance (QCM) sensor is still a high-precision surface sensing technique. Here, we described an immunoglobulin G (IgG) biofunctionalized QCM sensor for <italic xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>Staphylococcus aureus</i> (S. aureus) sensing application. In the case of the QCM sensor, a gold-titanium (Au/Ti) electrode layer was fabricated by magnetron sputtering and sequentially immobilized with thoil self-assembled monolayer (SAM) and IgG gripper. The selectivity test showed that the rabbit IgG-based QCM sensor exhibited a good sensitivity performance. IgG immobilization optimal experiment was conducted by adjusting parameters including immobilization time, temperature, buffer pH and IgG concentration. The lower limit of detection and sensitivity of the IgG-based QCM biosensor toward <italic xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>S. aureus</i> were suggested to be <inline-formula xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink> <tex-math notation=LaTeX>$1.0times {10}^{{4}}$ </tex-math></inline-formula> CFU/mL and 562.5 Hz/ng, respectively. Furthermore, the real-time monitoring and frequency response of IgG immobilization process and its sensitive adsorption behavior to <italic xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>S. aureus</i> were performed in droplet environment. By comparison, the average sensitivity of IgG-immobilized QCM sensor in liquid phase is 2-3.5 times higher than that in air. Overall, it is indicative that IgG-based QCM sensors do have great potential for biological monitoring applications." @default.
- W4285258416 created "2022-07-14" @default.
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- W4285258416 date "2022-06-15" @default.
- W4285258416 modified "2023-10-11" @default.
- W4285258416 title "Immunoglobulin-Immobilized Quartz Crystal Microbalance for Staphylococcus Aureus Real-Time Detection" @default.
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- W4285258416 doi "https://doi.org/10.1109/jsen.2022.3174161" @default.
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