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- W4285279027 abstract "This chapter introduces the 3D image reconstruction method for intracellular surgery using a robot-assisted microscopy system. First, a series of optical sections along the vertical direction is obtained by moving the microscope. A three-dimensional (3D) reconstruction model of the specimen is established after processing deconvolution, segmentation, and reconstruction. On the basis of 3D information, intracellular delivery can be achieved. The optimal delivery position can be acquired through geometry analysis based on the volume-based model. A robust controller that can compensate for external disturbances is utilized to manipulate micropipettes to deliver exogenous materials into single cells. Reconstruction accuracy experiments are performed on the cell nucleus, and the results are compared with confocal fluorescent microscopy. Experiments of intracellular nucleus delivery are further conducted using the proposed robotic microscopy system." @default.
- W4285279027 created "2022-07-14" @default.
- W4285279027 creator A5040437342 @default.
- W4285279027 date "2022-01-01" @default.
- W4285279027 modified "2023-09-27" @default.
- W4285279027 title "Three-dimensional image reconstruction and intracellular surgery" @default.
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- W4285279027 doi "https://doi.org/10.1016/b978-0-323-85259-3.00011-8" @default.
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