FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285384237> ?p ?o ?g. }

• W4285384237 endingPage "104493" @default.
• W4285384237 startingPage "104493" @default.
• W4285384237 abstract "DDX3, a member of the DEAD-box RNA helicase family and has highly conserved ATP-dependent RNA helicase activity, has important roles in RNA metabolism and innate anti-viral immune responses. In this study, five transcript variants of the DDX3 gene were cloned and characterized from rainbow trout (Oncorhynchus mykiss). These five transcript variants of DDX3 encoded proteins were 74.2 kDa (686 aa), 76.4 kDa (709 aa), 77.8 kDa (711 aa), 78.0 kDa (718 aa), and 78.8 kDa (729 aa) and the predicted isoelectric points were 6.91, 7.63, 7.63, 7.18, and 7.23, respectively. All rainbow trout DDX3 proteins contained two conserved RecA-like domains that were similar to the DDX3 protein reported in mammals. Phylogenetic analysis showed that the five cloned rainbow trout DDX3 were separate from mammals but clustered with fish, especially Northern pike (Esox lucius) and Nile tilapia (Oreochromis niloticus). RT-qPCR analysis showed that the DDX3 gene was broadly expressed in all tissues studied. The expression of DDX3 after infectious hematopoietic necrosis virus (IHNV) infection increased gradually after the early stage of IHNV infection, decreased gradually with the proliferation of IHNV in vivo (liver, spleen, and kidney), and was significantly decreased after the in vitro infection of epithelioma papulosum cyprini (EPC) and rainbow trout gonad cell line-2 (RTG-2) cell lines. We also found that rainbow trout DDX3 was significantly increased by a time-dependent mechanism after the poly I:C treatment of EPC and RTG cells; however no significant changes were observed with lipopolysaccharide (LPS) treatment. Knockdown of DDX3 by siRNA showed significantly increased IHNV replication in infected RTG cells. This study suggests that DDX3 has an important role in host defense against IHNV infection and these results may provide new insights into IHNV pathogenesis and antiviral drug research." @default.
• W4285384237 created "2022-07-14" @default.
• W4285384237 creator A5012263514 @default.
• W4285384237 creator A5027195409 @default.
• W4285384237 creator A5027584684 @default.
• W4285384237 creator A5050075582 @default.
• W4285384237 creator A5051391732 @default.
• W4285384237 date "2022-10-01" @default.
• W4285384237 modified "2023-09-28" @default.
• W4285384237 title "Identification and characterization of DEAD-box RNA helicase DDX3 in rainbow trout (Oncorhynchus mykiss) and its relationship with infectious hematopoietic necrosis virus infection" @default.
• W4285384237 cites W1538085012 @default.
• W4285384237 cites W1953495563 @default.
• W4285384237 cites W1980321878 @default.
• W4285384237 cites W1991436313 @default.
• W4285384237 cites W1993374643 @default.
• W4285384237 cites W2006427830 @default.
• W4285384237 cites W2012130783 @default.
• W4285384237 cites W2019300904 @default.
• W4285384237 cites W2032639971 @default.
• W4285384237 cites W2036164721 @default.
• W4285384237 cites W2058210634 @default.
• W4285384237 cites W2065118037 @default.
• W4285384237 cites W2072690167 @default.
• W4285384237 cites W2078188527 @default.
• W4285384237 cites W2092110803 @default.
• W4285384237 cites W2096113894 @default.
• W4285384237 cites W2096166831 @default.
• W4285384237 cites W2104538438 @default.
• W4285384237 cites W2123417984 @default.
• W4285384237 cites W2130014003 @default.
• W4285384237 cites W2142961497 @default.
• W4285384237 cites W2167655650 @default.
• W4285384237 cites W2171173836 @default.
• W4285384237 cites W2295641663 @default.
• W4285384237 cites W2309400418 @default.
• W4285384237 cites W2343385949 @default.
• W4285384237 cites W2344390401 @default.
• W4285384237 cites W2561919277 @default.
• W4285384237 cites W2591737580 @default.
• W4285384237 cites W2593053488 @default.
• W4285384237 cites W2741442634 @default.
• W4285384237 cites W2758711982 @default.
• W4285384237 cites W2793764828 @default.
• W4285384237 cites W2897967635 @default.
• W4285384237 cites W2898106022 @default.
• W4285384237 cites W2902293379 @default.
• W4285384237 cites W2906903513 @default.
• W4285384237 cites W2980904247 @default.
• W4285384237 cites W3007314268 @default.
• W4285384237 cites W3035353759 @default.
• W4285384237 cites W3166186004 @default.
• W4285384237 cites W3213530942 @default.
• W4285384237 doi "https://doi.org/10.1016/j.dci.2022.104493" @default.
• W4285384237 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35840014" @default.
• W4285384237 hasPublicationYear "2022" @default.
• W4285384237 type Work @default.
• W4285384237 citedByCount "1" @default.
• W4285384237 countsByYear W42853842372023 @default.
• W4285384237 crossrefType "journal-article" @default.
• W4285384237 hasAuthorship W4285384237A5012263514 @default.
• W4285384237 hasAuthorship W4285384237A5027195409 @default.
• W4285384237 hasAuthorship W4285384237A5027584684 @default.
• W4285384237 hasAuthorship W4285384237A5050075582 @default.
• W4285384237 hasAuthorship W4285384237A5051391732 @default.
• W4285384237 hasConcept C104317684 @default.
• W4285384237 hasConcept C153911025 @default.
• W4285384237 hasConcept C159047783 @default.
• W4285384237 hasConcept C161223559 @default.
• W4285384237 hasConcept C2778966499 @default.
• W4285384237 hasConcept C2779363728 @default.
• W4285384237 hasConcept C2909208804 @default.
• W4285384237 hasConcept C2994167347 @default.
• W4285384237 hasConcept C505870484 @default.
• W4285384237 hasConcept C54355233 @default.
• W4285384237 hasConcept C67705224 @default.
• W4285384237 hasConcept C68592252 @default.
• W4285384237 hasConcept C86803240 @default.
• W4285384237 hasConceptScore W4285384237C104317684 @default.
• W4285384237 hasConceptScore W4285384237C153911025 @default.
• W4285384237 hasConceptScore W4285384237C159047783 @default.
• W4285384237 hasConceptScore W4285384237C161223559 @default.
• W4285384237 hasConceptScore W4285384237C2778966499 @default.
• W4285384237 hasConceptScore W4285384237C2779363728 @default.
• W4285384237 hasConceptScore W4285384237C2909208804 @default.
• W4285384237 hasConceptScore W4285384237C2994167347 @default.
• W4285384237 hasConceptScore W4285384237C505870484 @default.
• W4285384237 hasConceptScore W4285384237C54355233 @default.
• W4285384237 hasConceptScore W4285384237C67705224 @default.
• W4285384237 hasConceptScore W4285384237C68592252 @default.
• W4285384237 hasConceptScore W4285384237C86803240 @default.
• W4285384237 hasFunder F4320335070 @default.
• W4285384237 hasFunder F4320335777 @default.
• W4285384237 hasFunder F4320338141 @default.
• W4285384237 hasLocation W42853842371 @default.
• W4285384237 hasLocation W42853842372 @default.
• W4285384237 hasOpenAccess W4285384237 @default.
• W4285384237 hasPrimaryLocation W42853842371 @default.
• W4285384237 hasRelatedWork W1963789155 @default.

• next