FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285386657> ?p ?o ?g. }

• W4285386657 abstract "Fungal mating-type loci ( MAT ) encode transcription factors (TFs) MAT1-1-1 and MAT1-2-1, which govern sexual reproduction as well as other developmental processes. In Penicillium chrysogenum , the major producer of the beta-lactam antibiotic penicillin, a recent chromatin immunoprecipitation followed by sequencing (ChIP-seq) analysis identified 254 genes as direct targets of MAT1-1-1, many of which encode thus far uncharacterized proteins. Here, we characterized one of the major targets of MAT1-1-1, the tom1 gene, which encodes a protein highly conserved within the group of Eurotiomycetes fungi. Using fluorescence microscopy, we demonstrated binding of MAT1-1-1 to the tom1 promoter by reporter gene analysis. Extensive electrophoretic mobility shift assays (EMSAs) further showed that the promoter sequence of tom1 is bound in vitro by both MAT1-1-1 and MAT1-2-1. This indicated an interaction between the two TFs, which was verified by yeast two-hybrid analysis. The sequence of tom1 carries a nuclear localization sequence, and indeed its nuclear localization was verified by fluorescence microscopy. The in vivo function of tom1 was investigated using tom1 deletion strains, as well as a complementing strain where the wild-type tom1 gene was reintroduced. We found a clear sporulation defect in the deletion strain, which became more evident when the fungi were grown at an elevated temperature of 31°C." @default.
• W4285386657 created "2022-07-14" @default.
• W4285386657 creator A5021457266 @default.
• W4285386657 creator A5084307476 @default.
• W4285386657 date "2022-07-14" @default.
• W4285386657 modified "2023-09-27" @default.
• W4285386657 title "The Penicillium chrysogenum tom1 Gene a Major Target of Transcription Factor MAT1-1-1 Encodes a Nuclear Protein Involved in Sporulation" @default.
• W4285386657 cites W1480690280 @default.
• W4285386657 cites W1578938503 @default.
• W4285386657 cites W1763660324 @default.
• W4285386657 cites W1860052163 @default.
• W4285386657 cites W1903792504 @default.
• W4285386657 cites W1913862483 @default.
• W4285386657 cites W1984397663 @default.
• W4285386657 cites W2002503145 @default.
• W4285386657 cites W2007149178 @default.
• W4285386657 cites W2015218321 @default.
• W4285386657 cites W2020465509 @default.
• W4285386657 cites W2031168303 @default.
• W4285386657 cites W2031768623 @default.
• W4285386657 cites W2044383585 @default.
• W4285386657 cites W2045444560 @default.
• W4285386657 cites W2053437843 @default.
• W4285386657 cites W2058877429 @default.
• W4285386657 cites W2058995008 @default.
• W4285386657 cites W2068527948 @default.
• W4285386657 cites W2081133825 @default.
• W4285386657 cites W2084282993 @default.
• W4285386657 cites W2094651657 @default.
• W4285386657 cites W2099540110 @default.
• W4285386657 cites W2119866322 @default.
• W4285386657 cites W2121814712 @default.
• W4285386657 cites W2123638539 @default.
• W4285386657 cites W2125142272 @default.
• W4285386657 cites W2126610283 @default.
• W4285386657 cites W2129944256 @default.
• W4285386657 cites W2130886513 @default.
• W4285386657 cites W2137250917 @default.
• W4285386657 cites W2138892630 @default.
• W4285386657 cites W2140952049 @default.
• W4285386657 cites W2150764377 @default.
• W4285386657 cites W2152359765 @default.
• W4285386657 cites W2159105557 @default.
• W4285386657 cites W2165443664 @default.
• W4285386657 cites W2169105416 @default.
• W4285386657 cites W2336068690 @default.
• W4285386657 cites W2436737319 @default.
• W4285386657 cites W2623012308 @default.
• W4285386657 cites W2734490137 @default.
• W4285386657 cites W274783243 @default.
• W4285386657 cites W2893337845 @default.
• W4285386657 cites W3003808794 @default.
• W4285386657 cites W3036502999 @default.
• W4285386657 cites W3046087333 @default.
• W4285386657 cites W3046198937 @default.
• W4285386657 cites W3132985874 @default.
• W4285386657 cites W1893572312 @default.
• W4285386657 doi "https://doi.org/10.3389/ffunb.2022.937023" @default.
• W4285386657 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37746180" @default.
• W4285386657 hasPublicationYear "2022" @default.
• W4285386657 type Work @default.
• W4285386657 citedByCount "0" @default.
• W4285386657 crossrefType "journal-article" @default.
• W4285386657 hasAuthorship W4285386657A5021457266 @default.
• W4285386657 hasAuthorship W4285386657A5084307476 @default.
• W4285386657 hasBestOaLocation W42853866571 @default.
• W4285386657 hasConcept C104317684 @default.
• W4285386657 hasConcept C138885662 @default.
• W4285386657 hasConcept C150194340 @default.
• W4285386657 hasConcept C153911025 @default.
• W4285386657 hasConcept C161733203 @default.
• W4285386657 hasConcept C179926584 @default.
• W4285386657 hasConcept C198348125 @default.
• W4285386657 hasConcept C2777270571 @default.
• W4285386657 hasConcept C41895202 @default.
• W4285386657 hasConcept C54355233 @default.
• W4285386657 hasConcept C86339819 @default.
• W4285386657 hasConcept C86803240 @default.
• W4285386657 hasConcept C89423630 @default.
• W4285386657 hasConceptScore W4285386657C104317684 @default.
• W4285386657 hasConceptScore W4285386657C138885662 @default.
• W4285386657 hasConceptScore W4285386657C150194340 @default.
• W4285386657 hasConceptScore W4285386657C153911025 @default.
• W4285386657 hasConceptScore W4285386657C161733203 @default.
• W4285386657 hasConceptScore W4285386657C179926584 @default.
• W4285386657 hasConceptScore W4285386657C198348125 @default.
• W4285386657 hasConceptScore W4285386657C2777270571 @default.
• W4285386657 hasConceptScore W4285386657C41895202 @default.
• W4285386657 hasConceptScore W4285386657C54355233 @default.
• W4285386657 hasConceptScore W4285386657C86339819 @default.
• W4285386657 hasConceptScore W4285386657C86803240 @default.
• W4285386657 hasConceptScore W4285386657C89423630 @default.
• W4285386657 hasFunder F4320320879 @default.
• W4285386657 hasLocation W42853866571 @default.
• W4285386657 hasLocation W42853866572 @default.
• W4285386657 hasOpenAccess W4285386657 @default.
• W4285386657 hasPrimaryLocation W42853866571 @default.
• W4285386657 hasRelatedWork W1818003313 @default.
• W4285386657 hasRelatedWork W1886625688 @default.
• W4285386657 hasRelatedWork W1920751942 @default.

• next