Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285389007> ?p ?o ?g. }
- W4285389007 abstract "Bacteriophages (phages) are capable of infecting specific bacteria, and therefore can be used as a biological control agent to control bacteria-induced animal, plant, and human diseases. In this study, two homolog phages (named PPAY and PPAT) that infect Pseudomonas aeruginosa PAO1 were isolated and characterized. The results of the phage plaque assay showed that PPAT plaques were transparent dots, while the PPAY plaques were translucent dots with a halo. Transmission electron microscopy results showed that PPAT (65 nm) and PPAY (60 nm) strains are similar in size and have an icosahedral head and a short tail. Therefore, these belong to the short-tailed phage family Podoviridae. One-step growth curves revealed the latent period of 20 min and burst time of 30 min for PPAT and PPAY. The burst size of PPAT (953 PFUs/infected cell) was higher than that of PPAY (457 PFUs/infected cell). Also, the adsorption rate constant of PPAT (5.97 × 10-7 ml/min) was higher than that of PPAY (1.32 × 10-7 ml/min) at 5 min. Whole-genome sequencing of phages was carried out using the Illumina HiSeq platform. The genomes of PPAT and PPAY have 54,888 and 50,154 bp, respectively. Only 17 of the 352 predicted ORFs of PPAT could be matched to homologous genes of known function. Likewise, among the 351 predicted ORFs of PPAY, only 18 ORFs could be matched to genes of established functions. Homology and evolutionary analysis indicated that PPAT and PPAY are closely related to PA11. The presence of tail fiber proteins in PPAY but not in PPAT may have contributed to the halo effect of its plaque spots. In all, PPAT and PPAY, newly discovered P. aeruginosa phages, showed growth inhibitory effects on bacteria and can be used for research and clinical purposes." @default.
- W4285389007 created "2022-07-14" @default.
- W4285389007 creator A5004360668 @default.
- W4285389007 creator A5016619443 @default.
- W4285389007 creator A5017157541 @default.
- W4285389007 creator A5030689354 @default.
- W4285389007 creator A5041777855 @default.
- W4285389007 creator A5045085415 @default.
- W4285389007 creator A5053004159 @default.
- W4285389007 creator A5054816741 @default.
- W4285389007 creator A5055030119 @default.
- W4285389007 creator A5061435321 @default.
- W4285389007 creator A5064175285 @default.
- W4285389007 creator A5068281654 @default.
- W4285389007 creator A5072950289 @default.
- W4285389007 creator A5083838646 @default.
- W4285389007 date "2022-07-14" @default.
- W4285389007 modified "2023-10-18" @default.
- W4285389007 title "Isolation and characterization of two homolog phages infecting Pseudomonas aeruginosa" @default.
- W4285389007 cites W129814170 @default.
- W4285389007 cites W1565042634 @default.
- W4285389007 cites W1700071556 @default.
- W4285389007 cites W1905970497 @default.
- W4285389007 cites W1963315039 @default.
- W4285389007 cites W1969077482 @default.
- W4285389007 cites W1976666054 @default.
- W4285389007 cites W1982383354 @default.
- W4285389007 cites W1987261946 @default.
- W4285389007 cites W1989261502 @default.
- W4285389007 cites W2000047180 @default.
- W4285389007 cites W2016145919 @default.
- W4285389007 cites W2026751874 @default.
- W4285389007 cites W2034744968 @default.
- W4285389007 cites W2044569644 @default.
- W4285389007 cites W2051133017 @default.
- W4285389007 cites W2056377972 @default.
- W4285389007 cites W2060774265 @default.
- W4285389007 cites W2063089818 @default.
- W4285389007 cites W2084433407 @default.
- W4285389007 cites W2095369542 @default.
- W4285389007 cites W2102474285 @default.
- W4285389007 cites W2104821962 @default.
- W4285389007 cites W2106263124 @default.
- W4285389007 cites W2109036909 @default.
- W4285389007 cites W2116788484 @default.
- W4285389007 cites W2125199856 @default.
- W4285389007 cites W2137149377 @default.
- W4285389007 cites W2140024588 @default.
- W4285389007 cites W2145652485 @default.
- W4285389007 cites W2154760096 @default.
- W4285389007 cites W2157402147 @default.
- W4285389007 cites W2163883372 @default.
- W4285389007 cites W2167054611 @default.
- W4285389007 cites W2174658119 @default.
- W4285389007 cites W2201787120 @default.
- W4285389007 cites W2269181712 @default.
- W4285389007 cites W2270093937 @default.
- W4285389007 cites W2299218023 @default.
- W4285389007 cites W2312974550 @default.
- W4285389007 cites W2346787319 @default.
- W4285389007 cites W2398550380 @default.
- W4285389007 cites W2461172035 @default.
- W4285389007 cites W2462034472 @default.
- W4285389007 cites W2518197527 @default.
- W4285389007 cites W2526803370 @default.
- W4285389007 cites W2603734291 @default.
- W4285389007 cites W2604443133 @default.
- W4285389007 cites W2606654615 @default.
- W4285389007 cites W2610642423 @default.
- W4285389007 cites W2618456216 @default.
- W4285389007 cites W2626068580 @default.
- W4285389007 cites W2735330212 @default.
- W4285389007 cites W2754895882 @default.
- W4285389007 cites W2756670863 @default.
- W4285389007 cites W2770467375 @default.
- W4285389007 cites W2774526471 @default.
- W4285389007 cites W2790264421 @default.
- W4285389007 cites W2799598773 @default.
- W4285389007 cites W2800051480 @default.
- W4285389007 cites W2801186127 @default.
- W4285389007 cites W2802355844 @default.
- W4285389007 cites W2805178232 @default.
- W4285389007 cites W2806176346 @default.
- W4285389007 cites W2808528175 @default.
- W4285389007 cites W2883707477 @default.
- W4285389007 cites W2883752853 @default.
- W4285389007 cites W2886418331 @default.
- W4285389007 cites W2892490545 @default.
- W4285389007 cites W2898102872 @default.
- W4285389007 cites W2903381708 @default.
- W4285389007 cites W2904354086 @default.
- W4285389007 cites W2909648129 @default.
- W4285389007 cites W2909758314 @default.
- W4285389007 cites W2916687996 @default.
- W4285389007 cites W2932297240 @default.
- W4285389007 cites W2936280925 @default.
- W4285389007 cites W2937347447 @default.
- W4285389007 cites W2942646416 @default.
- W4285389007 cites W2943878465 @default.
- W4285389007 cites W2948347995 @default.