Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285390868> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W4285390868 endingPage "487" @default.
- W4285390868 startingPage "476" @default.
- W4285390868 abstract "The accuracies of two molecular tests, PCR and loop-mediated isothermal amplification (LAMP) assay were compared with bacterial culture in detection of salmonella in poultry clinical samples. The icIR family transcriptional regulator gene was targeted and, out of 56 clinical specimens, 20 poultry field isolates were found positive for salmonella. Along with human isolates, reference strains of three different serovars, Salmonella Enteritidis (S. Enteritidis), S. Typhimurium and S. Infantis, were also tested. Eight different but genetically closely related bacterial genera (Klebsiella, Pseudomonas, Enterobacter, Campylobacter, Staphylococcus, Streptococcus, Escherichia and Pasteurella) were also used to evaluate the specificity of assay. The LAMP assay showed 80.8% sensitivity (95% CI, 0.66-0.95) and 100% specificity (95% CI, 0.71-1.00) when compared with microbiological culture and PCR, both with 100% sensitivity (95% CI, 0.87-1.00) and 100% specificity (95% CI, 0.71-1.00). High-resolution melt (HRM) curve analysis following PCR was able to differentiate between salmonella isolates based on their melting points, and all specimens were genotyped in three distinct HRM curve profiles. Each normalized melt curve profile represented one salmonella serotype and differences between the three melt profiles were correlated with nucleotide variations in the target gene sequences which demonstrated high discriminatory power of this technique. The colourimetric LAMP assay provided an alternative detection method capable of being used in the field, and showed analytical sensitivity for detection of 1 pg of salmonella DNA per reaction. The advantages and disadvantages of each test in detection of salmonella are discussed." @default.
- W4285390868 created "2022-07-14" @default.
- W4285390868 creator A5021422720 @default.
- W4285390868 creator A5033351779 @default.
- W4285390868 creator A5035271839 @default.
- W4285390868 date "2022-07-29" @default.
- W4285390868 modified "2023-09-27" @default.
- W4285390868 title "Comparison of colourimetric loop-mediated isothermal amplification (LAMP), PCR and high-resolution melt curve analysis and culture-based diagnostic assays in the detection of three salmonella serotypes in poultry" @default.
- W4285390868 cites W2006252131 @default.
- W4285390868 cites W2020898325 @default.
- W4285390868 cites W2029893373 @default.
- W4285390868 cites W2030938248 @default.
- W4285390868 cites W2032649327 @default.
- W4285390868 cites W2066306274 @default.
- W4285390868 cites W2077461808 @default.
- W4285390868 cites W2084454118 @default.
- W4285390868 cites W2101997581 @default.
- W4285390868 cites W2105275554 @default.
- W4285390868 cites W2106882534 @default.
- W4285390868 cites W2113355660 @default.
- W4285390868 cites W2165567383 @default.
- W4285390868 cites W2432391161 @default.
- W4285390868 cites W2523288704 @default.
- W4285390868 cites W2562404114 @default.
- W4285390868 cites W2767079245 @default.
- W4285390868 cites W2792325215 @default.
- W4285390868 cites W2794538558 @default.
- W4285390868 cites W2800826938 @default.
- W4285390868 cites W2807932594 @default.
- W4285390868 cites W2810095996 @default.
- W4285390868 cites W2935350986 @default.
- W4285390868 cites W2943007999 @default.
- W4285390868 cites W2986555333 @default.
- W4285390868 cites W2995308792 @default.
- W4285390868 cites W3002376498 @default.
- W4285390868 cites W3048376396 @default.
- W4285390868 cites W3091961119 @default.
- W4285390868 cites W3117339477 @default.
- W4285390868 cites W3156333852 @default.
- W4285390868 cites W3215296137 @default.
- W4285390868 doi "https://doi.org/10.1080/03079457.2022.2101916" @default.
- W4285390868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35833568" @default.
- W4285390868 hasPublicationYear "2022" @default.
- W4285390868 type Work @default.
- W4285390868 citedByCount "1" @default.
- W4285390868 countsByYear W42853908682023 @default.
- W4285390868 crossrefType "journal-article" @default.
- W4285390868 hasAuthorship W4285390868A5021422720 @default.
- W4285390868 hasAuthorship W4285390868A5033351779 @default.
- W4285390868 hasAuthorship W4285390868A5035271839 @default.
- W4285390868 hasConcept C10389963 @default.
- W4285390868 hasConcept C104317684 @default.
- W4285390868 hasConcept C2781065037 @default.
- W4285390868 hasConcept C2992956773 @default.
- W4285390868 hasConcept C34372108 @default.
- W4285390868 hasConcept C49105822 @default.
- W4285390868 hasConcept C523546767 @default.
- W4285390868 hasConcept C54355233 @default.
- W4285390868 hasConcept C552990157 @default.
- W4285390868 hasConcept C60635243 @default.
- W4285390868 hasConcept C84133368 @default.
- W4285390868 hasConcept C86803240 @default.
- W4285390868 hasConcept C89423630 @default.
- W4285390868 hasConceptScore W4285390868C10389963 @default.
- W4285390868 hasConceptScore W4285390868C104317684 @default.
- W4285390868 hasConceptScore W4285390868C2781065037 @default.
- W4285390868 hasConceptScore W4285390868C2992956773 @default.
- W4285390868 hasConceptScore W4285390868C34372108 @default.
- W4285390868 hasConceptScore W4285390868C49105822 @default.
- W4285390868 hasConceptScore W4285390868C523546767 @default.
- W4285390868 hasConceptScore W4285390868C54355233 @default.
- W4285390868 hasConceptScore W4285390868C552990157 @default.
- W4285390868 hasConceptScore W4285390868C60635243 @default.
- W4285390868 hasConceptScore W4285390868C84133368 @default.
- W4285390868 hasConceptScore W4285390868C86803240 @default.
- W4285390868 hasConceptScore W4285390868C89423630 @default.
- W4285390868 hasFunder F4320320961 @default.
- W4285390868 hasIssue "5" @default.
- W4285390868 hasLocation W42853908681 @default.
- W4285390868 hasLocation W42853908682 @default.
- W4285390868 hasOpenAccess W4285390868 @default.
- W4285390868 hasPrimaryLocation W42853908681 @default.
- W4285390868 hasRelatedWork W1977144460 @default.
- W4285390868 hasRelatedWork W2097366957 @default.
- W4285390868 hasRelatedWork W2161518991 @default.
- W4285390868 hasRelatedWork W2262744754 @default.
- W4285390868 hasRelatedWork W2792325215 @default.
- W4285390868 hasRelatedWork W2911973517 @default.
- W4285390868 hasRelatedWork W2990903192 @default.
- W4285390868 hasRelatedWork W4248728976 @default.
- W4285390868 hasRelatedWork W4285390868 @default.
- W4285390868 hasRelatedWork W4321434455 @default.
- W4285390868 hasVolume "51" @default.
- W4285390868 isParatext "false" @default.
- W4285390868 isRetracted "false" @default.
- W4285390868 workType "article" @default.