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- W4285392945 abstract "Electrical detection of messenger ribonucleic acid (mRNA) is a promising approach to enhancing transcriptomics and disease diagnostics because of its sensitivity, rapidity, and modularity. Reported here is a fast SARS-CoV-2 mRNA biosensor (<1 min) with a limit of detection of 100 aM and a linear sensitivity of 22 mV per molar decade. These figures of merit were obtained on photoresistlessly patterned monolayer graphene/SiO2 field-effect transistors (FETs) derived from commercial four-inch graphene on 90 nm of silicon dioxide on p-type silicon. Then, to facilitate mRNA hybridization, graphene sensing mesa were coated with an ultrathin sub-percolation threshold gold film for bonding 3′-thiolated single-stranded deoxyribonucleic acid (ssDNA) probes complementary to the SARS-CoV-2 nucleocapsid phosphoprotein (N) gene. Sub-percolated gold was used to minimize the distance between the graphene material and surface hybridization events. The liquid-transfer characteristics of the graphene/SiO2 FETs repeatedly shows correlation between the Dirac voltage and the copy number of polynucleotide. Ultrathin percolated gold films on graphene FETs facilitate two-dimensional electron gas (2DEG) mRNA biosensors for transcriptomic profiling." @default.
- W4285392945 created "2022-07-14" @default.
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- W4285392945 date "2022-07-13" @default.
- W4285392945 modified "2023-10-02" @default.
- W4285392945 title "Single-Stranded Deoxyribonucleic Acid Bonded to Sub-Percolated Gold Films on Monolayer Graphene Field-Effect Transistors as Coronavirus Ribonucleic Acid Sensors" @default.
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- W4285392945 doi "https://doi.org/10.1021/acsanm.2c01944" @default.
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