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• W4285394522 abstract "Abstract Understanding the immunological effects of chemotherapy is of great importance, especially now that we have entered an era where ever-increasing pre-clinical and clinical efforts are put into combining chemotherapy and immunotherapy to combat cancer. Single-cell RNA sequencing (scRNA-seq) has proved to be a powerful technique with a broad range of applications, studies evaluating drug effects in co-cultures of tumor and immune cells are however scarce. We treated a co-culture comprised of human colorectal cancer (CRC) cells and peripheral blood mononuclear cells (PBMCs) with the nucleoside analogue trifluridine (FTD) and used scRNA-seq to analyze posttreatment gene expression profiles in thousands of individual cancer and immune cells concurrently. ScRNA-seq recapitulated major mechanisms of action previously described for FTD and provided new insight into possible treatment-induced effects on T-cell mediated antitumor responses." @default.
• W4285394522 created "2022-07-14" @default.
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• W4285394522 date "2022-07-13" @default.
• W4285394522 modified "2023-10-18" @default.
• W4285394522 title "Single-cell transcriptional pharmacodynamics of trifluridine in a tumor-immune model" @default.
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• W4285394522 doi "https://doi.org/10.1038/s41598-022-16077-7" @default.
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• W4285394522 hasPublicationYear "2022" @default.
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