Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285405585> ?p ?o ?g. }
- W4285405585 endingPage "102259" @default.
- W4285405585 startingPage "102259" @default.
- W4285405585 abstract "The structural changes of airway smooth muscle (ASM) that characterize airway remodeling (AR) are crucial to the pathogenesis of asthma. During AR, ASM cells dedifferentiate from a quiescent to a proliferative, migratory, and secretory phenotype. Calcium (Ca2+) is a ubiquitous second messenger that regulates many cellular processes, including proliferation, migration, contraction, and metabolism. Furthermore, mitochondria have emerged as major Ca2+ signaling organelles that buffer Ca2+ through uptake by the mitochondrial Ca2+ uniporter and extrude it through the Na+/Ca2+ exchanger (NCLX/Slc8b1). Here, we show using mitochondrial Ca2+-sensitive dyes that NCLX only partially contributes to mitochondrial Ca2+ extrusion in ASM cells. Yet, NCLX is necessary for ASM cell proliferation and migration. Through cellular imaging, RNA-Seq, and biochemical assays, we demonstrate that NCLX regulates these processes by preventing mitochondrial Ca2+ overload and supporting store-operated Ca2+ entry, activation of Ca2+/calmodulin-dependent kinase II, and transcriptional and metabolic reprogramming. Using small animal respiratory mechanic measurements and immunohistochemistry, we show that smooth muscle-specific NCLX KO mice are protected against AR, fibrosis, and hyperresponsiveness in an experimental model of asthma. Our findings support NCLX as a potential therapeutic target in the treatment of asthma." @default.
- W4285405585 created "2022-07-14" @default.
- W4285405585 creator A5011499966 @default.
- W4285405585 creator A5014288991 @default.
- W4285405585 creator A5038670797 @default.
- W4285405585 creator A5053147342 @default.
- W4285405585 creator A5058980890 @default.
- W4285405585 creator A5061372270 @default.
- W4285405585 creator A5074073706 @default.
- W4285405585 creator A5079581156 @default.
- W4285405585 creator A5086084361 @default.
- W4285405585 creator A5087197408 @default.
- W4285405585 date "2022-08-01" @default.
- W4285405585 modified "2023-10-14" @default.
- W4285405585 title "The airway smooth muscle sodium/calcium exchanger NCLX is critical for airway remodeling and hyperresponsiveness in asthma" @default.
- W4285405585 cites W1077661664 @default.
- W4285405585 cites W1572192011 @default.
- W4285405585 cites W1598892278 @default.
- W4285405585 cites W1875615308 @default.
- W4285405585 cites W1918205979 @default.
- W4285405585 cites W1934528477 @default.
- W4285405585 cites W1963740134 @default.
- W4285405585 cites W1969154199 @default.
- W4285405585 cites W1969709153 @default.
- W4285405585 cites W1970468800 @default.
- W4285405585 cites W1989505831 @default.
- W4285405585 cites W1991078304 @default.
- W4285405585 cites W2002791298 @default.
- W4285405585 cites W2010932100 @default.
- W4285405585 cites W2018956640 @default.
- W4285405585 cites W2019832656 @default.
- W4285405585 cites W2028630600 @default.
- W4285405585 cites W2035968737 @default.
- W4285405585 cites W2039521726 @default.
- W4285405585 cites W2050297509 @default.
- W4285405585 cites W2057517217 @default.
- W4285405585 cites W2067609194 @default.
- W4285405585 cites W2067841051 @default.
- W4285405585 cites W2070805734 @default.
- W4285405585 cites W2083990515 @default.
- W4285405585 cites W2084375915 @default.
- W4285405585 cites W2087726370 @default.
- W4285405585 cites W2094105256 @default.
- W4285405585 cites W2098276532 @default.
- W4285405585 cites W2099036724 @default.
- W4285405585 cites W2101840868 @default.
- W4285405585 cites W2108211053 @default.
- W4285405585 cites W2109380465 @default.
- W4285405585 cites W2111450699 @default.
- W4285405585 cites W2114104545 @default.
- W4285405585 cites W2118025157 @default.
- W4285405585 cites W2120362293 @default.
- W4285405585 cites W2122005351 @default.
- W4285405585 cites W2122374115 @default.
- W4285405585 cites W2127292113 @default.
- W4285405585 cites W2127803322 @default.
- W4285405585 cites W2130116522 @default.
- W4285405585 cites W2130410032 @default.
- W4285405585 cites W2132031140 @default.
- W4285405585 cites W2137692993 @default.
- W4285405585 cites W2142188572 @default.
- W4285405585 cites W2146066778 @default.
- W4285405585 cites W2146252494 @default.
- W4285405585 cites W2153426958 @default.
- W4285405585 cites W2154431984 @default.
- W4285405585 cites W2158331725 @default.
- W4285405585 cites W2163317648 @default.
- W4285405585 cites W2164247949 @default.
- W4285405585 cites W2165536982 @default.
- W4285405585 cites W2168342464 @default.
- W4285405585 cites W2169456326 @default.
- W4285405585 cites W2343452853 @default.
- W4285405585 cites W2344396284 @default.
- W4285405585 cites W2482176933 @default.
- W4285405585 cites W2489374855 @default.
- W4285405585 cites W2517861725 @default.
- W4285405585 cites W2518073968 @default.
- W4285405585 cites W2542623541 @default.
- W4285405585 cites W2589392509 @default.
- W4285405585 cites W2609751917 @default.
- W4285405585 cites W2763526200 @default.
- W4285405585 cites W2782770196 @default.
- W4285405585 cites W2795364183 @default.
- W4285405585 cites W2883314903 @default.
- W4285405585 cites W2913565756 @default.
- W4285405585 cites W2970114486 @default.
- W4285405585 cites W2979467650 @default.
- W4285405585 cites W3040358661 @default.
- W4285405585 cites W3042073243 @default.
- W4285405585 cites W3083084296 @default.
- W4285405585 cites W3085056329 @default.
- W4285405585 cites W3133427814 @default.
- W4285405585 cites W3190273141 @default.
- W4285405585 cites W3198444904 @default.
- W4285405585 cites W4200013068 @default.
- W4285405585 cites W4233491463 @default.
- W4285405585 cites W643497125 @default.
- W4285405585 doi "https://doi.org/10.1016/j.jbc.2022.102259" @default.