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- W4285490626 abstract "Abstract Aliarcobacter butzleri (formerly known as Arcobacter butzleri) is an emerging foodborne zoonotic pathogen that establishes in vitro endosymbiotic relationships with Acanthamoeba castellanii, a free-living amoeba. Previously, we described that this bacterium acts as an endocytobiont of A. castellanii, surviving for at least 10 days in absence of bacterial replication. Thus, the aim of this study was to evaluate the ability of A. butzleri to survive as a long-term endosymbiont of A. castellanii for 30 days in two models of symbiotic interaction with A. castellanii: i) endosymbiotic culture followed by gentamicin protection assay and ii) transwell co-culture assay. The results allow us to conclude that A. butzleri is capable of surviving as an endosymbiont of A. castellanii for at least 30 days, without multiplying, under controlled laboratory conditions. In addition, in the absence of nutrients and as both microorganisms remain in the same culture, separated by semi-permeable membranes, A. castellanii does not promote the survival of A. butzleri, nor does it multiply. Our findings suggest that the greater survival capacity of A. butzleri is associated with their endosymbiont status inside A. castellanii, pointing out the complexity of this type of symbiotic relationship." @default.
- W4285490626 created "2022-07-15" @default.
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- W4285490626 date "2022-07-15" @default.
- W4285490626 modified "2023-10-16" @default.
- W4285490626 title "Long-term survive of Aliarcobacter butzleri in two models symbiotic interaction with Acanthamoeba castellanii" @default.
- W4285490626 doi "https://doi.org/10.21203/rs.3.rs-1832763/v1" @default.
- W4285490626 hasPublicationYear "2022" @default.
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