Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285493348> ?p ?o ?g. }
- W4285493348 abstract "A bright and photostable fluorescent dye with a disulfide (S-S) linker and maleimide group (Rho594-S2-mal), as cleavable and reactive sites, was synthesized and conjugated with anti-GFP nanobodies (NB). The binding of EGFP (FRET donor) with anti-GFP NB labeled with one or two Rho594-S2-mal residues was studied in vitro and in cellulo. The linker was cleaved with dithiothreitol recovering the donor (FP) signal. The bioconjugates (FP-NB-dye) were applied in FRET-FLIM assays, confocal imaging, and superresolution STED microscopy." @default.
- W4285493348 created "2022-07-15" @default.
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- W4285493348 date "2022-08-16" @default.
- W4285493348 modified "2023-10-18" @default.
- W4285493348 title "Cleavable Linker Incorporation into a Synthetic Dye‐Nanobody‐Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy" @default.
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- W4285493348 doi "https://doi.org/10.1002/cbic.202200395" @default.
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