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- W4285498798 abstract "• A new strategy for pyruvate detection in fermentation progress was developed based on the consumption of H 2 O 2 through the non-enzymatic decarboxylation. • An enhanced amperometric H 2 O 2 biosensor was designed using HRP enzyme-inorganic hybrid nanoflowers and thiol graphene modified Au disk electrode. • The biosensor was used for the determination of high concentration pyruvate in real microbial fermentation processes without pretreatment. We report a facile strategy for the detection of pyruvate in fermentation progress based on a non-enzymatic decarboxylation reaction of pyruvate with hydrogen peroxide (H 2 O 2 ), coupled with a horseradish peroxidase (HRP)-incorporating nanomaterial as an enzyme element to replace conventional pyruvate oxidase (POX). HRP-Cu 3 (PO 4 ) 2 •3H 2 O nanoflowers (HRP-CuP NFs) and thiol graphene (tG) were immobilized onto an Au disk electrode via layer-by-layer assembly to construct an improved amperometric H 2 O 2 biosensor. The as-prepared HRP-CuP NF/tG/Au electrode exhibited a high electrocatalytic performance toward H 2 O 2 , showing a sensitive response of 67.6 μA mM -1 cm -2 , as well as a linear range from 0.1-8.2 mM with a detection limit (LOD) of 0.06 mM (S/N=3). Furthermore, the resulting biosensor also demonstrated a linear range for pyruvate detection from 1.0 to 8.0 mM with LOD of 0.3 mM (S/N=3), under a working potential of -0.3 V. Finally, the biosensor was successfully used for the determination of pyruvate in real microbial fermentation processes without pretreatment, showing an excellent correlation coefficient compared to the standard HPLC assay with RSD values of 3.03-6.45%. This work provides a new approach for the design and preparation of portable sensors for indirect pyruvate detection with high performance, low cost, and easy operation." @default.
- W4285498798 created "2022-07-15" @default.
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- W4285498798 date "2022-09-01" @default.
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- W4285498798 title "A novel strategy for the detection of pyruvate in fermentation processes based on well-distributed enzyme-inorganic hybrid nanoflowers on thiol graphene modified gold electrodes" @default.
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- W4285498798 doi "https://doi.org/10.1016/j.electacta.2022.140855" @default.
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