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- W4285607704 abstract "With the improvement of sanitation, the infection rate of hookworm is greatly reduced and the severe infected case is rarely reported. Combined morphological and molecular biological examinations, a severe hookworm infection patient was diagnosed in Department of Laboratorial Examination, Quanzhou First Affiliated Hospital of Fujian Medical University. The morphological methods such as direct fecal smear microscopy, saturated brine flotation and hookworm larvae culture methods were used to identify the eggs and larvae from stool samples of the patient. There were a large number of hookworm eggs in patient's stool samples, and the average count was 60 840 per gram by modified Kato method, which belonged to severe hookworm infection. Meanwhile, to distinguish the hookworm species, the semi-nested RT-PCR assay was employed to detect hookworm internal transcribed spacer series from eggs in patient's stool samples, and the result showed that the hookworm species was confirmed to be Necator americanus.随着我国卫生条件的改善，钩虫的感染率得到了大幅度的降低，重症感染的患者已极其罕见。福建医科大学附属泉州第一医院检验科通过形态学检查和分子生物学方法，确诊了1例极重症钩虫感染的患者。该患者粪便直接涂片法和钩蚴培养法可见大量虫卵，从其虫卵和幼虫形态学上判断为钩虫，改良加藤法计数虫卵为每克粪便60 840个，属于极重度感染状态；同时还提取了粪便中虫卵的DNA，采用半巢式RT-PCR对其进行虫种鉴定，结果显示为美洲钩虫。." @default.
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- W4285607704 date "2022-06-28" @default.
- W4285607704 modified "2023-09-29" @default.
- W4285607704 title "A case of severe hookworm infection." @default.
- W4285607704 doi "https://doi.org/10.11817/j.issn.1672-7347.2022.210593" @default.
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