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- W4285687553 abstract "The following protocol has been optimized for image analysis-based quantification of collagen within FFPE spheroid samples. It is run on a Leica ST5020 automated stainer. This protocol can be adapted to manual staining. PURPOSE: Trichrome stains are used to differentiate collagen from other tissues. They are usually nuclear and cytoplasmic stains, followed by differentiation, then followed by a collagen fiber stain. Trichrome stains are useful for indicating fibrotic changes. PRINCIPLE: The staining reaction is based on the differential effect of acid dye on collagen and other tissue components." @default.
- W4285687553 created "2022-07-17" @default.
- W4285687553 creator A5052827209 @default.
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- W4285687553 date "2020-04-27" @default.
- W4285687553 modified "2023-09-28" @default.
- W4285687553 title "Masson's Trichrome Staining Protocol for FFPE spheroids and microtissues v1" @default.
- W4285687553 doi "https://doi.org/10.17504/protocols.io.bfnsjmee" @default.
- W4285687553 hasPublicationYear "2020" @default.
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