FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285804328> ?p ?o ?g. }

• W4285804328 endingPage "888" @default.
• W4285804328 startingPage "881" @default.
• W4285804328 abstract "To construct three-dimensional (3D) pre-vascularized microstructures and explore the promoting effect of human fibroblasts (HFs) on the sprout and migration of human umbilical vein endothelial cells (HUVECs) in 3D co-culture system.HUVECs and HFs were cultured and the 3rd to 5th generation cells were selected for subsequent experiments. In 2D co-culture system, HFs were stained with PKH26 and the cell density was fixed, which co-cultured with HUVECs in different ratios (1∶4, 1∶1, 4∶1) and inoculation methods (HUVECs inoculation at 48 hours after HFs, direct mixed inoculation). Then the formation of vascular like structures was observed under fluorescence microscope. In 3D co-culture system, HUVECs and HFs were labeled with green fluorescent protein and red fluorescent protein by lentivirus transfection, respectively. They were inoculated on porous micro-carriers followed by dynamically culturing in rotating bottles to prepare HF, HUVEC, HF-EC, or HF&EC microstructures. The cell growth in microstructures was testing by low permeability crystal violet staining. Subsequently, the microstructures were embedded in fibrin gel and the cell growth and adhesion in HF and HUVEC microstructures were observed by laser confocal microscopy. Laser confocal microscope were also used to observe the sprouts of 4 kinds of microstructures, as well as the cell composition, the number and length of sprouts from HF-EC and HF&EC microstructures. HFs conditioned medium was prepared to observe its effect on sprouts of HUVEC microstructures with DMEM as control group.In 2D co-culture system, HFs pre-culturing was helpful to the formation and stability of vascular like structures, and the best effect was when the ratio of two kinds of cells was 1∶1. In 3D co-culture system, it was found that the cells grew well on micro-carriers and had the ability of pre-vascularization. HUVEC microstructures did not sprout, but HF, HF-EC, and HF&EC microstructures could which indicated a good vascularization ability. The HF-EC microstructures were superior to HF&EC microstructures in terms of sprouts length and number ( P<0.05). The tubes sprouting from co-cultured group were composed of HFs and HUVECs, and HF microstructures migration preceded HUVEC microstructures always, and their migration trajectories were the same. HUVEC microstructures could sprout when cultured in HFs conditioned media.HF-HUVEC pre-vascularized microstructures can be prepared by pre-culturing HFs before HUVECs and with the cell ratio at 1∶1 in a rotating bottle. In 3D co-culture system, HFs can promote and guide the sprout of HUVECs.构建三维预血管化微组织，探索三维共培养体系中人成纤维细胞（human fibroblasts，HFs）对人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVECs）出芽和迁移的促进作用。.取HUVECs和HFs培养传代至3～5代进行实验。在二维共培养体系中，将HFs行PKH26染色且固定细胞密度后，与HUVECs以不同比例（1∶4、1∶1、4∶1）以及不同接种方式（两种细胞间隔48 h顺序接种、直接混合接种）共培养，荧光显微镜下观察血管网络形成情况。构建三维共培养体系，通过慢病毒转染分别用绿色和红色荧光蛋白标记HUVECs和HFs，将标记细胞接种于多孔微载体上，采用转瓶动态培养，分别制备HF微组织、HUVEC微组织、HF-EC微组织和HF&EC微组织；低渗结晶紫染色法观测微组织中细胞生长情况。将各微组织包埋于纤维蛋白凝胶中，激光共聚焦显微镜下观察HF微组织和HUVEC微组织中细胞生长贴附情况，4种微组织出芽情况以及HF-EC微组织、HF&EC微组织芽体细胞组成、出芽数量及芽体长度。制备HFs条件培养基，观察其对HUVEC微组织出芽的影响，以正常培养基培养作为对照。.二维共培养时HFs预培养有助于血管网状结构形成与稳定，且当两种细胞比例为1∶1时效果最佳。三维共培养时各微组织上细胞生长良好，HUVEC微组织无出芽，HF微组织、HF-EC微组织、HF&EC微组织均能出芽，具备良好血管化能力。HF-EC微组织芽体长度及出芽数目均优于HF&EC微组织（ P<0.05）。共培养微组织中HF微组织先于HUVEC微组织出芽，两者出芽轨迹一致，芽体由HFs和HUVECs组成。在HFs条件培养基中，HUVEC微组织也能出芽。.在转瓶中按1∶1比例顺序接种HFs和HUVECs可制备HF-HUVEC预血管化微组织，且在三维共培养中HFs能够引导和促进HUVECs出芽及迁移。." @default.
• W4285804328 created "2022-07-19" @default.
• W4285804328 creator A5016855459 @default.
• W4285804328 creator A5033997863 @default.
• W4285804328 creator A5042290117 @default.
• W4285804328 creator A5056355344 @default.
• W4285804328 creator A5068478712 @default.
• W4285804328 date "2022-07-15" @default.
• W4285804328 modified "2023-10-05" @default.
• W4285804328 title "[Effect of fibroblasts on promoting the sprout and migration of endothelial cells in three-dimensional pre-vascularized microstructures]." @default.
• W4285804328 cites W1257643254 @default.
• W4285804328 cites W1988787256 @default.
• W4285804328 cites W2042875291 @default.
• W4285804328 cites W2050025717 @default.
• W4285804328 cites W2072341418 @default.
• W4285804328 cites W2076021612 @default.
• W4285804328 cites W2110014836 @default.
• W4285804328 cites W2193257617 @default.
• W4285804328 cites W2575570481 @default.
• W4285804328 cites W2578602994 @default.
• W4285804328 cites W2618307797 @default.
• W4285804328 cites W2786917169 @default.
• W4285804328 cites W2883475305 @default.
• W4285804328 cites W2889107532 @default.
• W4285804328 cites W2891724652 @default.
• W4285804328 cites W2959830553 @default.
• W4285804328 cites W3088120666 @default.
• W4285804328 cites W3100232185 @default.
• W4285804328 doi "https://doi.org/10.7507/1002-1892.202203028" @default.
• W4285804328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35848186" @default.
• W4285804328 hasPublicationYear "2022" @default.
• W4285804328 type Work @default.
• W4285804328 citedByCount "0" @default.
• W4285804328 crossrefType "journal-article" @default.
• W4285804328 hasAuthorship W4285804328A5016855459 @default.
• W4285804328 hasAuthorship W4285804328A5033997863 @default.
• W4285804328 hasAuthorship W4285804328A5042290117 @default.
• W4285804328 hasAuthorship W4285804328A5056355344 @default.
• W4285804328 hasAuthorship W4285804328A5068478712 @default.
• W4285804328 hasConcept C120665830 @default.
• W4285804328 hasConcept C121332964 @default.
• W4285804328 hasConcept C12554922 @default.
• W4285804328 hasConcept C136009344 @default.
• W4285804328 hasConcept C142724271 @default.
• W4285804328 hasConcept C1491633281 @default.
• W4285804328 hasConcept C153911025 @default.
• W4285804328 hasConcept C169274487 @default.
• W4285804328 hasConcept C185592680 @default.
• W4285804328 hasConcept C202751555 @default.
• W4285804328 hasConcept C2777411675 @default.
• W4285804328 hasConcept C2777834122 @default.
• W4285804328 hasConcept C2779178360 @default.
• W4285804328 hasConcept C54355233 @default.
• W4285804328 hasConcept C553184892 @default.
• W4285804328 hasConcept C55493867 @default.
• W4285804328 hasConcept C62112901 @default.
• W4285804328 hasConcept C71924100 @default.
• W4285804328 hasConcept C74864618 @default.
• W4285804328 hasConcept C8010536 @default.
• W4285804328 hasConcept C81885089 @default.
• W4285804328 hasConcept C86803240 @default.
• W4285804328 hasConcept C87976508 @default.
• W4285804328 hasConcept C91881484 @default.
• W4285804328 hasConcept C95444343 @default.
• W4285804328 hasConceptScore W4285804328C120665830 @default.
• W4285804328 hasConceptScore W4285804328C121332964 @default.
• W4285804328 hasConceptScore W4285804328C12554922 @default.
• W4285804328 hasConceptScore W4285804328C136009344 @default.
• W4285804328 hasConceptScore W4285804328C142724271 @default.
• W4285804328 hasConceptScore W4285804328C1491633281 @default.
• W4285804328 hasConceptScore W4285804328C153911025 @default.
• W4285804328 hasConceptScore W4285804328C169274487 @default.
• W4285804328 hasConceptScore W4285804328C185592680 @default.
• W4285804328 hasConceptScore W4285804328C202751555 @default.
• W4285804328 hasConceptScore W4285804328C2777411675 @default.
• W4285804328 hasConceptScore W4285804328C2777834122 @default.
• W4285804328 hasConceptScore W4285804328C2779178360 @default.
• W4285804328 hasConceptScore W4285804328C54355233 @default.
• W4285804328 hasConceptScore W4285804328C553184892 @default.
• W4285804328 hasConceptScore W4285804328C55493867 @default.
• W4285804328 hasConceptScore W4285804328C62112901 @default.
• W4285804328 hasConceptScore W4285804328C71924100 @default.
• W4285804328 hasConceptScore W4285804328C74864618 @default.
• W4285804328 hasConceptScore W4285804328C8010536 @default.
• W4285804328 hasConceptScore W4285804328C81885089 @default.
• W4285804328 hasConceptScore W4285804328C86803240 @default.
• W4285804328 hasConceptScore W4285804328C87976508 @default.
• W4285804328 hasConceptScore W4285804328C91881484 @default.
• W4285804328 hasConceptScore W4285804328C95444343 @default.
• W4285804328 hasIssue "7" @default.
• W4285804328 hasLocation W42858043281 @default.
• W4285804328 hasOpenAccess W4285804328 @default.
• W4285804328 hasPrimaryLocation W42858043281 @default.
• W4285804328 hasRelatedWork W2039516224 @default.
• W4285804328 hasRelatedWork W2041730814 @default.
• W4285804328 hasRelatedWork W2047824326 @default.
• W4285804328 hasRelatedWork W2065748262 @default.
• W4285804328 hasRelatedWork W2101657929 @default.

• next