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• W4285807717 abstract "Initial retention of the bacterium Escherichia coli on model poly(dimethylsiloxane) (PDMS) surfaces was studied as a function of substrate bulk and surface mechanical stiffness values. Our reference PDMS system was designed such that out of the parameters that govern bacterial adhesion only the mechanical stiffness was systematically varied. This was achieved by varying the crosslinking density of PDMS. Following crosslinking, we performed Soxhlet extraction of non-crosslinked, free chains to rule out their effect on bacterial response. Bulk moduli were assessed by dynamic mechanical analysis at 1 rad sec−1 frequency and the values obtained ranged between 0.03 and 1.8 MPa. The increase in crosslink density resulted in increasing surface modulus, as measured by atomic force microscopy, with values ranging between 0.7 and 9 MPa. The number of bacteria retained was then assessed. We observed a decreasing trend with the increase of both bulk and surface mechanical stiffnesses down to a limit corresponding to the Young’s modulus of the bacterial cell surface. For higher values than this threshold, the number of retained bacteria remained constant. We tentatively explain this observation by considering conformal overlay of bacterial and material surfaces." @default.
• W4285807717 created "2022-07-19" @default.
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• W4285807717 date "2022-09-01" @default.
• W4285807717 modified "2023-10-18" @default.
• W4285807717 title "Initial bacterial retention on polydimethylsiloxane of various stiffnesses: The relevance of modulus (mis)match" @default.
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• W4285807717 doi "https://doi.org/10.1016/j.colsurfb.2022.112709" @default.
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