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• W4285988170 abstract "ABSTRACT The existence of a placental microbiota is under debate. The human placenta has historically been considered sterile and microbial colonization has been associated with adverse pregnancy outcomes. Yet, recent investigations using DNA sequencing reported a microbiota in human placentas from typical term pregnancies. However, this detected microbiota could represent background DNA contamination. Using fifteen publicly available 16S rRNA gene datasets, existing data were uniformly re-analyzed. 16S rRNA gene Amplicon Sequence Variants (ASVs) identified as Lactobacillus were highly abundant in eight of fifteen studies. However, the prevalence of Lactobacillus , a typical vaginal bacterium, was clearly driven by bacterial contamination from vaginal delivery and background DNA. After removal of likely DNA contaminants, Lactobacillus ASVs were highly abundant in only one of five studies for which data analysis could be restricted to placentas from term cesarean deliveries. A six study sub-analysis targeting the 16S rRNA gene V4 hypervariable region demonstrated that bacterial profiles of placental samples and technical controls share principal bacterial ASVs and that placental samples clustered primarily by study origin and mode of delivery. Across studies, placentas from typical term pregnancies did not share a consistent bacterial taxonomic signal. Contemporary DNA- based evidence does not support the existence of a placental microbiota. IMPORTANCE Early-gestational microbial influences on human development are unclear. By applying DNA sequencing technologies to placental tissue, bacterial DNA signals were observed, leading some to conclude that a live bacterial placental microbiome exists in typical term pregnancy. However, the low-biomass nature of the proposed microbiome and high sensitivity of current DNA sequencing technologies indicate that the signal may alternatively derive from environmental or delivery-associated bacterial DNA contamination. Here we address these alternatives with a re- analysis of 16S rRNA gene sequencing data from 15 publicly available placental datasets. After identical DADA2 pipeline processing of the raw data, subanalyses were performed to control for mode of delivery and environmental DNA contamination. Both environment and mode of delivery profoundly influenced the bacterial DNA signal from term-delivered placentas. Aside from these contamination-associated signals, consistency was lacking across studies. Thus, placentas delivered at term are unlikely to be the original source of observed bacterial DNA signals." @default.
• W4285988170 created "2022-07-20" @default.
• W4285988170 creator A5004264500 @default.
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• W4285988170 date "2022-07-20" @default.
• W4285988170 modified "2023-10-18" @default.
• W4285988170 title "Is there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets" @default.
• W4285988170 cites W1576783408 @default.
• W4285988170 cites W1607357496 @default.
• W4285988170 cites W1910713243 @default.
• W4285988170 cites W1966460156 @default.
• W4285988170 cites W1980404240 @default.
• W4285988170 cites W1983943311 @default.
• W4285988170 cites W1985231600 @default.
• W4285988170 cites W1996610163 @default.
• W4285988170 cites W1996956539 @default.
• W4285988170 cites W1998735142 @default.
• W4285988170 cites W1999537838 @default.
• W4285988170 cites W2000858187 @default.
• W4285988170 cites W2009829189 @default.
• W4285988170 cites W2009901678 @default.
• W4285988170 cites W2013371150 @default.
• W4285988170 cites W2021895713 @default.
• W4285988170 cites W2023506796 @default.
• W4285988170 cites W2025568822 @default.
• W4285988170 cites W2028246039 @default.
• W4285988170 cites W2028761993 @default.
• W4285988170 cites W2034285706 @default.
• W4285988170 cites W2039164411 @default.
• W4285988170 cites W2056228221 @default.
• W4285988170 cites W2056279562 @default.
• W4285988170 cites W2056825845 @default.
• W4285988170 cites W2059122601 @default.
• W4285988170 cites W2062011235 @default.
• W4285988170 cites W2069779096 @default.
• W4285988170 cites W2080283023 @default.
• W4285988170 cites W2085727838 @default.
• W4285988170 cites W2093782142 @default.
• W4285988170 cites W2095473593 @default.
• W4285988170 cites W2099888884 @default.
• W4285988170 cites W2101004214 @default.
• W4285988170 cites W2103090529 @default.
• W4285988170 cites W2103296919 @default.
• W4285988170 cites W2106574527 @default.
• W4285988170 cites W2111975989 @default.
• W4285988170 cites W2126467946 @default.
• W4285988170 cites W2131042289 @default.
• W4285988170 cites W2133371096 @default.
• W4285988170 cites W2138111878 @default.
• W4285988170 cites W2158847005 @default.
• W4285988170 cites W2169474803 @default.
• W4285988170 cites W2170486072 @default.
• W4285988170 cites W22033954 @default.
• W4285988170 cites W2285991887 @default.
• W4285988170 cites W2312480680 @default.
• W4285988170 cites W2319290978 @default.
• W4285988170 cites W2326987869 @default.
• W4285988170 cites W2333095042 @default.
• W4285988170 cites W2339073512 @default.
• W4285988170 cites W2346788382 @default.
• W4285988170 cites W2394897061 @default.
• W4285988170 cites W2401404581 @default.
• W4285988170 cites W2406250479 @default.
• W4285988170 cites W2462800864 @default.
• W4285988170 cites W2474696439 @default.
• W4285988170 cites W2494627911 @default.
• W4285988170 cites W2514615451 @default.
• W4285988170 cites W2536667468 @default.
• W4285988170 cites W2557682897 @default.
• W4285988170 cites W2560183723 @default.
• W4285988170 cites W2580445261 @default.
• W4285988170 cites W2592244930 @default.
• W4285988170 cites W2592367925 @default.
• W4285988170 cites W2593726167 @default.
• W4285988170 cites W2599849933 @default.
• W4285988170 cites W2600682639 @default.
• W4285988170 cites W2602772235 @default.
• W4285988170 cites W2605927576 @default.
• W4285988170 cites W2609646274 @default.
• W4285988170 cites W2611957242 @default.
• W4285988170 cites W2615504694 @default.
• W4285988170 cites W2617080443 @default.
• W4285988170 cites W262938957 @default.
• W4285988170 cites W2724320608 @default.
• W4285988170 cites W2735951541 @default.
• W4285988170 cites W2736755748 @default.
• W4285988170 cites W2747926456 @default.
• W4285988170 cites W2749272636 @default.
• W4285988170 cites W2751811618 @default.
• W4285988170 cites W2752349849 @default.
• W4285988170 cites W2763922522 @default.
• W4285988170 cites W2783072167 @default.
• W4285988170 cites W2783736669 @default.
• W4285988170 cites W2783909471 @default.
• W4285988170 cites W2784164089 @default.

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