Matches in SemOpenAlex for { <https://semopenalex.org/work/W4285992523> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4285992523 endingPage "S24" @default.
- W4285992523 startingPage "S24" @default.
- W4285992523 abstract "The Human Cell Atlas project has previously catalogued the cell types in human skin derived from adult, fetal skin and inflammatory skin diseases. However, we lack a comprehensive understanding of how these cell types are organized spatially within the tissue and how they differ qualitatively and across anatomical sites and in cutaneous malignancy. To address these questions, we have analyzed single cell RNA sequencing of 120,000 cells from diverse healthy anatomical sites and compared to 60 000 cells from basal cell carcinoma (BCC) - the most common malignancy in humans. We have integrated this with spatial transcriptomic data across 24 samples to map the spatial patterns of cellular localization at 50 micron resolution. Subsequently, in situ sequencing of 12 samples using a panel of 200 genes allowed us to define cellular-resolution maps of identity for these tissue states. We find that whilst cellular identities are broadly conserved across anatomical sites, cells retain a memory of embryological origin. We find T cells to be more abundant in the skin of the head compared to the trunk and limbs with differential expression of T cell markers. Additionally, we find that fibroblast subpopulations differ subtly in their identity across anatomical sites and exhibit distinct patterns of spatial localization within the dermis. In BCC, we observe a characteristic spatial arrangement of fibroblast populations at the interface of the epithelial and mesenchymal elements of the tumor. We dissect the relative importance of intrinsic transcriptional programs and spatial context in the specification of fibroblast identity through RNA FISH analysis in the immediate transition to cell culture conditions. Our findings provide key insights into the cellular landscape of skin from different healthy anatomical locations and BCC and suggest that the interface between epithelial and mesenchymal elements of the tumor may offer new therapeutic targets for the treatment of cutaneous malignancy." @default.
- W4285992523 created "2022-07-21" @default.
- W4285992523 creator A5013573794 @default.
- W4285992523 creator A5021226680 @default.
- W4285992523 creator A5029049559 @default.
- W4285992523 creator A5032093545 @default.
- W4285992523 creator A5066508811 @default.
- W4285992523 creator A5085311539 @default.
- W4285992523 date "2022-08-01" @default.
- W4285992523 modified "2023-09-28" @default.
- W4285992523 title "140 Dissecting the cellular landscape of human skin across anatomical sites and in cutaneous malignancy through single cell transcriptomics and in situ sequencing" @default.
- W4285992523 doi "https://doi.org/10.1016/j.jid.2022.05.146" @default.
- W4285992523 hasPublicationYear "2022" @default.
- W4285992523 type Work @default.
- W4285992523 citedByCount "0" @default.
- W4285992523 crossrefType "journal-article" @default.
- W4285992523 hasAuthorship W4285992523A5013573794 @default.
- W4285992523 hasAuthorship W4285992523A5021226680 @default.
- W4285992523 hasAuthorship W4285992523A5029049559 @default.
- W4285992523 hasAuthorship W4285992523A5032093545 @default.
- W4285992523 hasAuthorship W4285992523A5066508811 @default.
- W4285992523 hasAuthorship W4285992523A5085311539 @default.
- W4285992523 hasBestOaLocation W42859925231 @default.
- W4285992523 hasConcept C104317684 @default.
- W4285992523 hasConcept C105702510 @default.
- W4285992523 hasConcept C142724271 @default.
- W4285992523 hasConcept C1491633281 @default.
- W4285992523 hasConcept C150194340 @default.
- W4285992523 hasConcept C151730666 @default.
- W4285992523 hasConcept C162317418 @default.
- W4285992523 hasConcept C189014844 @default.
- W4285992523 hasConcept C2777459323 @default.
- W4285992523 hasConcept C2777624698 @default.
- W4285992523 hasConcept C2779343474 @default.
- W4285992523 hasConcept C2779399171 @default.
- W4285992523 hasConcept C2780381497 @default.
- W4285992523 hasConcept C54355233 @default.
- W4285992523 hasConcept C71924100 @default.
- W4285992523 hasConcept C81885089 @default.
- W4285992523 hasConcept C86803240 @default.
- W4285992523 hasConceptScore W4285992523C104317684 @default.
- W4285992523 hasConceptScore W4285992523C105702510 @default.
- W4285992523 hasConceptScore W4285992523C142724271 @default.
- W4285992523 hasConceptScore W4285992523C1491633281 @default.
- W4285992523 hasConceptScore W4285992523C150194340 @default.
- W4285992523 hasConceptScore W4285992523C151730666 @default.
- W4285992523 hasConceptScore W4285992523C162317418 @default.
- W4285992523 hasConceptScore W4285992523C189014844 @default.
- W4285992523 hasConceptScore W4285992523C2777459323 @default.
- W4285992523 hasConceptScore W4285992523C2777624698 @default.
- W4285992523 hasConceptScore W4285992523C2779343474 @default.
- W4285992523 hasConceptScore W4285992523C2779399171 @default.
- W4285992523 hasConceptScore W4285992523C2780381497 @default.
- W4285992523 hasConceptScore W4285992523C54355233 @default.
- W4285992523 hasConceptScore W4285992523C71924100 @default.
- W4285992523 hasConceptScore W4285992523C81885089 @default.
- W4285992523 hasConceptScore W4285992523C86803240 @default.
- W4285992523 hasIssue "8" @default.
- W4285992523 hasLocation W42859925231 @default.
- W4285992523 hasOpenAccess W4285992523 @default.
- W4285992523 hasPrimaryLocation W42859925231 @default.
- W4285992523 hasRelatedWork W150990555 @default.
- W4285992523 hasRelatedWork W1990963425 @default.
- W4285992523 hasRelatedWork W2303877139 @default.
- W4285992523 hasRelatedWork W2398349929 @default.
- W4285992523 hasRelatedWork W2785713700 @default.
- W4285992523 hasRelatedWork W2947570218 @default.
- W4285992523 hasRelatedWork W3157307916 @default.
- W4285992523 hasRelatedWork W3176611933 @default.
- W4285992523 hasRelatedWork W3184775979 @default.
- W4285992523 hasRelatedWork W193816056 @default.
- W4285992523 hasVolume "142" @default.
- W4285992523 isParatext "false" @default.
- W4285992523 isRetracted "false" @default.
- W4285992523 workType "article" @default.