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- W4286009574 abstract "Arginine methylation is emerging as a key post-translational modification involved in a large range of biological processes. Its study in tissue is often limited by the lack of a specific antibody recognizing the target arginine residue. Proximity ligation assay (PLA) was originally developed to study protein/protein interactions. Here, we describe in detail a PLA protocol dedicated to the detection of arginine methylation that we applied to the glucocorticoid receptor (GR). Having previously shown that PRMT5 dimethylates GRs in cells, we used PLA with a pan symmetrical dimethyl antibody and an anti-GR antibody to measure GR methylation in breast tumors. We demonstrate that PLA offers a unique approach to measure arginine methylation of a target protein, even when the site of methylation has not been identified. This technique could be extended to other post-translational modifications where effective pan antibodies are available. Hence, we detail the PLA technology used to detect arginine methylation in fixed tissue using GR as an example." @default.
- W4286009574 created "2022-07-21" @default.
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- W4286009574 date "2022-07-20" @default.
- W4286009574 modified "2023-10-16" @default.
- W4286009574 title "Proximity Ligation Assay Allows the Detection, Localization, and Quantification of Protein Arginine Methylation in Fixed Tissue" @default.
- W4286009574 doi "https://doi.org/10.3791/64294" @default.
- W4286009574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35938827" @default.
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