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- W4286208105 endingPage "e1010607" @default.
- W4286208105 startingPage "e1010607" @default.
- W4286208105 abstract "Metals such as copper (Cu) and zinc (Zn) are important trace elements that can affect bacterial cell physiology but can also intoxicate bacteria at high concentrations. Discrete genetic systems for management of Cu and Zn efflux have been described in several bacterial pathogens, including streptococci. However, insight into molecular cross-talk between systems for Cu and Zn management in bacteria that drive metal detoxification, is limited. Here, we describe a biologically consequential cross-system effect of metal management in group B Streptococcus (GBS) governed by the Cu-responsive copY regulator in response to Zn. RNAseq analysis of wild-type (WT) and copY -deficient GBS subjected to metal stress revealed unique transcriptional links between the systems for Cu and Zn detoxification. We show that the Cu-sensing role of CopY extends beyond Cu and enables CopY to regulate Cu and Zn stress responses that effect changes in gene function for central cellular processes, including riboflavin synthesis. CopY also supported GBS intracellular survival in human macrophages and virulence during disseminated infection in mice. In addition, we show a novel role for CovR in modulating GBS resistance to Zn intoxication. Identification of the Zn resistome of GBS using TraDIS revealed a suite of genes essential for GBS growth in metal stress. Several of the genes identified are novel to systems that support bacterial survival in metal stress and represent a diverse set of mechanisms that underpin microbial metal homeostasis during cell stress. Overall, this study reveals a new and important mechanism of cross-system complexity driven by CopY in bacteria to regulate cellular management of metal stress and survival." @default.
- W4286208105 created "2022-07-21" @default.
- W4286208105 creator A5028113161 @default.
- W4286208105 creator A5058445985 @default.
- W4286208105 creator A5060677832 @default.
- W4286208105 date "2022-07-21" @default.
- W4286208105 modified "2023-09-27" @default.
- W4286208105 title "Regulatory cross-talk supports resistance to Zn intoxication in Streptococcus" @default.
- W4286208105 cites W1532273383 @default.
- W4286208105 cites W1657109975 @default.
- W4286208105 cites W1905908362 @default.
- W4286208105 cites W1914010951 @default.
- W4286208105 cites W1968136212 @default.
- W4286208105 cites W1974213037 @default.
- W4286208105 cites W1996641418 @default.
- W4286208105 cites W1997114098 @default.
- W4286208105 cites W2030621944 @default.
- W4286208105 cites W2031512086 @default.
- W4286208105 cites W2034162042 @default.
- W4286208105 cites W2075045948 @default.
- W4286208105 cites W2075350111 @default.
- W4286208105 cites W2075840772 @default.
- W4286208105 cites W2084760062 @default.
- W4286208105 cites W2087862053 @default.
- W4286208105 cites W2089172583 @default.
- W4286208105 cites W2090032009 @default.
- W4286208105 cites W2097270286 @default.
- W4286208105 cites W2103740237 @default.
- W4286208105 cites W2108244474 @default.
- W4286208105 cites W2110871531 @default.
- W4286208105 cites W2112274740 @default.
- W4286208105 cites W2114908128 @default.
- W4286208105 cites W2119612735 @default.
- W4286208105 cites W2119685853 @default.
- W4286208105 cites W2125460607 @default.
- W4286208105 cites W2129677884 @default.
- W4286208105 cites W2130046639 @default.
- W4286208105 cites W2134157602 @default.
- W4286208105 cites W2134526812 @default.
- W4286208105 cites W2141533388 @default.
- W4286208105 cites W2141631862 @default.
- W4286208105 cites W2143403799 @default.
- W4286208105 cites W2144244803 @default.
- W4286208105 cites W2149176191 @default.
- W4286208105 cites W2150603656 @default.
- W4286208105 cites W2153038986 @default.
- W4286208105 cites W2159930438 @default.
- W4286208105 cites W2162098634 @default.
- W4286208105 cites W2168276387 @default.
- W4286208105 cites W2168420558 @default.
- W4286208105 cites W2179438025 @default.
- W4286208105 cites W2217737927 @default.
- W4286208105 cites W226258753 @default.
- W4286208105 cites W2262816559 @default.
- W4286208105 cites W2301985416 @default.
- W4286208105 cites W2310902680 @default.
- W4286208105 cites W2460540891 @default.
- W4286208105 cites W2463297289 @default.
- W4286208105 cites W2501022724 @default.
- W4286208105 cites W2507288897 @default.
- W4286208105 cites W2562542841 @default.
- W4286208105 cites W2563859344 @default.
- W4286208105 cites W2567666868 @default.
- W4286208105 cites W2582651744 @default.
- W4286208105 cites W2593870782 @default.
- W4286208105 cites W2597506409 @default.
- W4286208105 cites W2620480148 @default.
- W4286208105 cites W2766292714 @default.
- W4286208105 cites W2766440777 @default.
- W4286208105 cites W2767797639 @default.
- W4286208105 cites W2768024065 @default.
- W4286208105 cites W2791130909 @default.
- W4286208105 cites W2794368593 @default.
- W4286208105 cites W2802779526 @default.
- W4286208105 cites W2805794351 @default.
- W4286208105 cites W2806579973 @default.
- W4286208105 cites W2829277060 @default.
- W4286208105 cites W2885564449 @default.
- W4286208105 cites W2901531046 @default.
- W4286208105 cites W2921330802 @default.
- W4286208105 cites W2941007283 @default.
- W4286208105 cites W2964865257 @default.
- W4286208105 cites W2969837772 @default.
- W4286208105 cites W2980145670 @default.
- W4286208105 cites W2991323459 @default.
- W4286208105 cites W3003687593 @default.
- W4286208105 cites W3031020505 @default.
- W4286208105 cites W3043085355 @default.
- W4286208105 cites W3043665879 @default.
- W4286208105 cites W3046037115 @default.
- W4286208105 cites W3080393910 @default.
- W4286208105 cites W3102581385 @default.
- W4286208105 cites W3109532390 @default.
- W4286208105 cites W3126868050 @default.
- W4286208105 cites W3135534550 @default.
- W4286208105 cites W3161474249 @default.
- W4286208105 cites W3180306812 @default.
- W4286208105 cites W3197792426 @default.