Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286210003> ?p ?o ?g. }
- W4286210003 endingPage "7996" @default.
- W4286210003 startingPage "7996" @default.
- W4286210003 abstract "Pollen fertility plays an important role in the application of heterosis in wheat (Triticum aestivum L.). However, the key genes and mechanisms underlying pollen abortion in K-type male sterility remain unclear. TAA1a is an essential gene for pollen development in wheat. Here, we explored the mechanism involved in its transcriptional regulation during pollen development, focusing on a 1315-bp promoter region. Several cis-acting elements were identified in the TAA1a promoter, including binding motifs for Arabidopsis thaliana AtAMS and AtMYB103 (CANNTG and CCAACC, respectively). Evolutionary analysis indicated that TaTDRL and TaMYB103 were the T. aestivum homologs of AtAMS and AtMYB103, respectively, and encoded nucleus-localized transcription factors containing 557 and 352 amino acids, respectively. TaTDRL and TaMYB103 were specifically expressed in wheat anthers, and their expression levels were highest in the early uninucleate stage; this expression pattern was consistent with that of TAA1a. Meanwhile, we found that TaTDRL and TaMYB03 directly interacted, as evidenced by yeast two-hybrid and bimolecular fluorescence complementation assays, while yeast one-hybrid and dual-luciferase assays revealed that both TaTDRL and TaMYB103 could bind the TAA1a promoter and synergistically increase its transcriptional activity. Furthermore, TaTDRL-EAR and TaMYB103-EAR transgenic Arabidopsis plants displayed abnormal microspore morphology, reduced pollen viability, and lowered seed setting rates. Additionally, the expression of AtMS2, a TAA1a homolog, was significantly lower in the two repressor lines than in the corresponding overexpression lines or WT plants. In summary, we identified a potential transcriptional regulatory mechanism associated with wheat pollen development." @default.
- W4286210003 created "2022-07-21" @default.
- W4286210003 creator A5003573683 @default.
- W4286210003 creator A5029703168 @default.
- W4286210003 creator A5048510498 @default.
- W4286210003 creator A5059871359 @default.
- W4286210003 creator A5062585998 @default.
- W4286210003 creator A5063569207 @default.
- W4286210003 creator A5075318484 @default.
- W4286210003 creator A5083275411 @default.
- W4286210003 creator A5091499181 @default.
- W4286210003 date "2022-07-20" @default.
- W4286210003 modified "2023-10-13" @default.
- W4286210003 title "The Transcription Factors TaTDRL and TaMYB103 Synergistically Activate the Expression of TAA1a in Wheat, Which Positively Regulates the Development of Microspore in Arabidopsis" @default.
- W4286210003 cites W1528794162 @default.
- W4286210003 cites W1590268200 @default.
- W4286210003 cites W1955916017 @default.
- W4286210003 cites W1965326888 @default.
- W4286210003 cites W1968719148 @default.
- W4286210003 cites W1986535717 @default.
- W4286210003 cites W1987943951 @default.
- W4286210003 cites W1991985109 @default.
- W4286210003 cites W1993753717 @default.
- W4286210003 cites W2013892862 @default.
- W4286210003 cites W2035908878 @default.
- W4286210003 cites W2039181172 @default.
- W4286210003 cites W2046269809 @default.
- W4286210003 cites W2046479301 @default.
- W4286210003 cites W2051386929 @default.
- W4286210003 cites W2061940962 @default.
- W4286210003 cites W2073857657 @default.
- W4286210003 cites W2076956347 @default.
- W4286210003 cites W2077620996 @default.
- W4286210003 cites W2077744980 @default.
- W4286210003 cites W2078966926 @default.
- W4286210003 cites W2084972544 @default.
- W4286210003 cites W2085815047 @default.
- W4286210003 cites W2086375197 @default.
- W4286210003 cites W2086491289 @default.
- W4286210003 cites W2093019594 @default.
- W4286210003 cites W2093931964 @default.
- W4286210003 cites W2097378319 @default.
- W4286210003 cites W2099267861 @default.
- W4286210003 cites W2100945610 @default.
- W4286210003 cites W2107277218 @default.
- W4286210003 cites W2108769403 @default.
- W4286210003 cites W2111336569 @default.
- W4286210003 cites W2111926354 @default.
- W4286210003 cites W2114891756 @default.
- W4286210003 cites W2117313985 @default.
- W4286210003 cites W2123058210 @default.
- W4286210003 cites W2124866062 @default.
- W4286210003 cites W2124881211 @default.
- W4286210003 cites W2127854400 @default.
- W4286210003 cites W2128615486 @default.
- W4286210003 cites W2133378187 @default.
- W4286210003 cites W2137358559 @default.
- W4286210003 cites W2139081121 @default.
- W4286210003 cites W2147056179 @default.
- W4286210003 cites W2155801118 @default.
- W4286210003 cites W2157257895 @default.
- W4286210003 cites W2157380319 @default.
- W4286210003 cites W2158018108 @default.
- W4286210003 cites W2163515652 @default.
- W4286210003 cites W2167786533 @default.
- W4286210003 cites W2168965248 @default.
- W4286210003 cites W2192080449 @default.
- W4286210003 cites W2280233867 @default.
- W4286210003 cites W2327276016 @default.
- W4286210003 cites W2333574083 @default.
- W4286210003 cites W2402243019 @default.
- W4286210003 cites W2472071648 @default.
- W4286210003 cites W2482918106 @default.
- W4286210003 cites W2596323082 @default.
- W4286210003 cites W2753944295 @default.
- W4286210003 cites W2883166674 @default.
- W4286210003 cites W2906737283 @default.
- W4286210003 cites W2940621239 @default.
- W4286210003 cites W2972058935 @default.
- W4286210003 cites W3000406741 @default.
- W4286210003 cites W3014350479 @default.
- W4286210003 cites W3130393632 @default.
- W4286210003 doi "https://doi.org/10.3390/ijms23147996" @default.
- W4286210003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35887343" @default.
- W4286210003 hasPublicationYear "2022" @default.
- W4286210003 type Work @default.
- W4286210003 citedByCount "2" @default.
- W4286210003 countsByYear W42862100032022 @default.
- W4286210003 countsByYear W42862100032023 @default.
- W4286210003 crossrefType "journal-article" @default.
- W4286210003 hasAuthorship W4286210003A5003573683 @default.
- W4286210003 hasAuthorship W4286210003A5029703168 @default.
- W4286210003 hasAuthorship W4286210003A5048510498 @default.
- W4286210003 hasAuthorship W4286210003A5059871359 @default.
- W4286210003 hasAuthorship W4286210003A5062585998 @default.
- W4286210003 hasAuthorship W4286210003A5063569207 @default.
- W4286210003 hasAuthorship W4286210003A5075318484 @default.
- W4286210003 hasAuthorship W4286210003A5083275411 @default.