FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286271010> ?p ?o ?g. }

• W4286271010 endingPage "3836" @default.
• W4286271010 startingPage "3828" @default.
• W4286271010 abstract "This paper explored the protective effect of total flavonoids of Rhododendron simsii(TFR) on focal cerebral ischemia-reperfusion injury(CIRI) in rats and its relationship with the store-operated calcium entry(SOCE) pathway regulated by stromal intera-ction molecule(STIM) and calcium release-activated calcium modulator(Orai).Rats were randomly assigned into the sham group, model(middle cerebral artery occlusion, MCAO) group, TFR(60 mg·kg~(-1)) group, TFR(60 mg·kg~(-1))+SOCE pathway inhibitor 2-aminoethoxydiphenyl borate(2-APB, 2.5 mg·kg~(-1)) group, and 2-APB(2.5 mg·kg~(-1)) group.The rats in the sham group and MCAO group were administrated with normal saline, and those in the TFR group and TFR+2-APB group were administrated with TFR(60 mg·kg~(-1)) by gavage for 14 days until sampling.The rats in the 2-APB group and TFR+2-APB group were intraperitoneally injected with 2-APB(2.5 mg·kg~(-1)) after operation.The levels of interleukin-1(IL-1), interleukin-6(IL-6), and tumor necrosis factor-alpha(TNF-α) in serum were measured by ELISA.The cerebral infarction and the pathological status of ischemic brain tissue were detected via TTC staining and HE staining, respectively.The protein and mRNA levels of STIM1, STIM2, Orai1, cysteinyl aspartate specific proteinase 3(caspase-3), and protein kinase B(PKB) in brain tissue were respectively determined by Western blot and RT-qPCR.The growth of brain neurons in each group was observed via immunofluorescence method.The results showed that compared with the MCAO group, TFR lowered the levels of IL-1, IL-6 and TNF-α in serum and the score of neurological function, ameliorated the pathological injury of brain tissue, and decreased the infarct size.Moreover, TFR up-regulated the mRNA and protein levels of STIM1, STIM2, Orai1, and PKB, down-regulated those of caspase-3 in brain tissue, and increased the double-labeled positive cells under fluorescence microscope.However, the above effects were significantly weakened by the addition of 2-APB, a SOCE inhibitor.The results suggested that TFR may play a protective role against focal cerebral ischemia-reperfusion injury by up-regulating the expression of SOCE-related signal molecules, promoting neurogenesis around the ischemic area, improving the survival state of neurons, and redu-cing the activity of inflammatory mediators." @default.
• W4286271010 created "2022-07-21" @default.
• W4286271010 creator A5004752549 @default.
• W4286271010 creator A5008801056 @default.
• W4286271010 creator A5017825677 @default.
• W4286271010 creator A5018454282 @default.
• W4286271010 creator A5020553354 @default.
• W4286271010 creator A5041888295 @default.
• W4286271010 creator A5059288102 @default.
• W4286271010 creator A5062077638 @default.
• W4286271010 creator A5065618488 @default.
• W4286271010 creator A5084281774 @default.
• W4286271010 date "2022-07-01" @default.
• W4286271010 modified "2023-09-28" @default.
• W4286271010 title "[Protective effect of total flavonoids of Rhododendron simsii on focal cerebral ischemia-reperfusion injury through SOCE pathway in rats]." @default.
• W4286271010 doi "https://doi.org/10.19540/j.cnki.cjcmm.20220323.401" @default.
• W4286271010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35850841" @default.
• W4286271010 hasPublicationYear "2022" @default.
• W4286271010 type Work @default.
• W4286271010 citedByCount "0" @default.
• W4286271010 crossrefType "journal-article" @default.
• W4286271010 hasAuthorship W4286271010A5004752549 @default.
• W4286271010 hasAuthorship W4286271010A5008801056 @default.
• W4286271010 hasAuthorship W4286271010A5017825677 @default.
• W4286271010 hasAuthorship W4286271010A5018454282 @default.
• W4286271010 hasAuthorship W4286271010A5020553354 @default.
• W4286271010 hasAuthorship W4286271010A5041888295 @default.
• W4286271010 hasAuthorship W4286271010A5059288102 @default.
• W4286271010 hasAuthorship W4286271010A5062077638 @default.
• W4286271010 hasAuthorship W4286271010A5065618488 @default.
• W4286271010 hasAuthorship W4286271010A5084281774 @default.
• W4286271010 hasConcept C104317684 @default.
• W4286271010 hasConcept C126322002 @default.
• W4286271010 hasConcept C134018914 @default.
• W4286271010 hasConcept C17991360 @default.
• W4286271010 hasConcept C181199279 @default.
• W4286271010 hasConcept C185592680 @default.
• W4286271010 hasConcept C2776415932 @default.
• W4286271010 hasConcept C2780114680 @default.
• W4286271010 hasConcept C541997718 @default.
• W4286271010 hasConcept C55493867 @default.
• W4286271010 hasConcept C56928146 @default.
• W4286271010 hasConcept C71924100 @default.
• W4286271010 hasConcept C98274493 @default.
• W4286271010 hasConceptScore W4286271010C104317684 @default.
• W4286271010 hasConceptScore W4286271010C126322002 @default.
• W4286271010 hasConceptScore W4286271010C134018914 @default.
• W4286271010 hasConceptScore W4286271010C17991360 @default.
• W4286271010 hasConceptScore W4286271010C181199279 @default.
• W4286271010 hasConceptScore W4286271010C185592680 @default.
• W4286271010 hasConceptScore W4286271010C2776415932 @default.
• W4286271010 hasConceptScore W4286271010C2780114680 @default.
• W4286271010 hasConceptScore W4286271010C541997718 @default.
• W4286271010 hasConceptScore W4286271010C55493867 @default.
• W4286271010 hasConceptScore W4286271010C56928146 @default.
• W4286271010 hasConceptScore W4286271010C71924100 @default.
• W4286271010 hasConceptScore W4286271010C98274493 @default.
• W4286271010 hasIssue "14" @default.
• W4286271010 hasLocation W42862710101 @default.
• W4286271010 hasOpenAccess W4286271010 @default.
• W4286271010 hasPrimaryLocation W42862710101 @default.
• W4286271010 hasRelatedWork W10529949 @default.
• W4286271010 hasRelatedWork W11522807 @default.
• W4286271010 hasRelatedWork W11779203 @default.
• W4286271010 hasRelatedWork W1326407 @default.
• W4286271010 hasRelatedWork W14670481 @default.
• W4286271010 hasRelatedWork W16562739 @default.
• W4286271010 hasRelatedWork W1764682 @default.
• W4286271010 hasRelatedWork W23355895 @default.
• W4286271010 hasRelatedWork W4043397 @default.
• W4286271010 hasRelatedWork W5661899 @default.
• W4286271010 hasVolume "47" @default.
• W4286271010 isParatext "false" @default.
• W4286271010 isRetracted "false" @default.
• W4286271010 workType "article" @default.