Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286470928> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W4286470928 abstract "Abstract CRISPR-mediated transcriptional activation (CRISPRa) is a powerful technology for inducing gene expression from endogenous loci with exciting applications in high throughput gain-of-function genomic screens and the engineering of cell-based models. However, current strategies for generating potent, stable, CRISPRa-competent cell-lines present limitations for the broad utility of this approach. Here, we provide a high-efficiency, self-selecting CRISPRa enrichment strategy, which combined with piggyBac transposon technology enables rapid production of CRISPRa-ready cell populations compatible with a variety of downstream assays. We complement this with a new, optimized guide RNA scaffold that significantly enhances CRISPRa functionality. Finally, we describe a novel, synthetic guide RNA tool set that enables transient, population-wide gene activation when used with the self-selecting CRISPRa system. Taken together, this versatile platform greatly enhances the potential for CRISPRa across a wide variety of cellular contexts." @default.
- W4286470928 created "2022-07-22" @default.
- W4286470928 creator A5004485524 @default.
- W4286470928 creator A5019157937 @default.
- W4286470928 creator A5031334804 @default.
- W4286470928 creator A5057026801 @default.
- W4286470928 creator A5069469851 @default.
- W4286470928 date "2022-07-22" @default.
- W4286470928 modified "2023-10-16" @default.
- W4286470928 title "A versatile, high-efficiency platform for CRISPR-based gene activation" @default.
- W4286470928 cites W2050870581 @default.
- W4286470928 cites W2068427957 @default.
- W4286470928 cites W2101749257 @default.
- W4286470928 cites W2123784922 @default.
- W4286470928 cites W2127862779 @default.
- W4286470928 cites W2141157874 @default.
- W4286470928 cites W2145668963 @default.
- W4286470928 cites W2152505144 @default.
- W4286470928 cites W2203807603 @default.
- W4286470928 cites W2204875887 @default.
- W4286470928 cites W2209402024 @default.
- W4286470928 cites W2221809773 @default.
- W4286470928 cites W2268496068 @default.
- W4286470928 cites W2608102145 @default.
- W4286470928 cites W2624845852 @default.
- W4286470928 cites W2735132087 @default.
- W4286470928 cites W2765809583 @default.
- W4286470928 cites W2772524563 @default.
- W4286470928 cites W2803581084 @default.
- W4286470928 cites W2902259724 @default.
- W4286470928 cites W2904226822 @default.
- W4286470928 cites W2936686072 @default.
- W4286470928 cites W2947777875 @default.
- W4286470928 cites W3000424462 @default.
- W4286470928 cites W3026436747 @default.
- W4286470928 cites W3031515954 @default.
- W4286470928 cites W3032340252 @default.
- W4286470928 cites W3045914787 @default.
- W4286470928 cites W3125923516 @default.
- W4286470928 cites W4224210604 @default.
- W4286470928 doi "https://doi.org/10.1101/2022.07.21.501015" @default.
- W4286470928 hasPublicationYear "2022" @default.
- W4286470928 type Work @default.
- W4286470928 citedByCount "0" @default.
- W4286470928 crossrefType "posted-content" @default.
- W4286470928 hasAuthorship W4286470928A5004485524 @default.
- W4286470928 hasAuthorship W4286470928A5019157937 @default.
- W4286470928 hasAuthorship W4286470928A5031334804 @default.
- W4286470928 hasAuthorship W4286470928A5057026801 @default.
- W4286470928 hasAuthorship W4286470928A5069469851 @default.
- W4286470928 hasBestOaLocation W42864709281 @default.
- W4286470928 hasConcept C104317684 @default.
- W4286470928 hasConcept C127413603 @default.
- W4286470928 hasConcept C132455925 @default.
- W4286470928 hasConcept C183696295 @default.
- W4286470928 hasConcept C41008148 @default.
- W4286470928 hasConcept C54355233 @default.
- W4286470928 hasConcept C70721500 @default.
- W4286470928 hasConcept C86803240 @default.
- W4286470928 hasConcept C98108389 @default.
- W4286470928 hasConceptScore W4286470928C104317684 @default.
- W4286470928 hasConceptScore W4286470928C127413603 @default.
- W4286470928 hasConceptScore W4286470928C132455925 @default.
- W4286470928 hasConceptScore W4286470928C183696295 @default.
- W4286470928 hasConceptScore W4286470928C41008148 @default.
- W4286470928 hasConceptScore W4286470928C54355233 @default.
- W4286470928 hasConceptScore W4286470928C70721500 @default.
- W4286470928 hasConceptScore W4286470928C86803240 @default.
- W4286470928 hasConceptScore W4286470928C98108389 @default.
- W4286470928 hasLocation W42864709281 @default.
- W4286470928 hasOpenAccess W4286470928 @default.
- W4286470928 hasPrimaryLocation W42864709281 @default.
- W4286470928 hasRelatedWork W2098022344 @default.
- W4286470928 hasRelatedWork W2116553552 @default.
- W4286470928 hasRelatedWork W2209344393 @default.
- W4286470928 hasRelatedWork W2344454439 @default.
- W4286470928 hasRelatedWork W2794852126 @default.
- W4286470928 hasRelatedWork W2898208578 @default.
- W4286470928 hasRelatedWork W2900771990 @default.
- W4286470928 hasRelatedWork W2943124855 @default.
- W4286470928 hasRelatedWork W2953290252 @default.
- W4286470928 hasRelatedWork W3086851723 @default.
- W4286470928 isParatext "false" @default.
- W4286470928 isRetracted "false" @default.
- W4286470928 workType "article" @default.