Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286500378> ?p ?o ?g. }
- W4286500378 abstract "Summary Chromosome alignment to the spindle equator is a hallmark of mitosis that is thought to promote chromosome segregation fidelity in metazoans. Yet, chromosome alignment is only indirectly supervised by the spindle assembly checkpoint (SAC) as a byproduct of chromosome bi-orientation, and the consequences of defective chromosome alignment remain unclear. Here we investigated how human cells respond to chromosome alignment defects of distinct molecular nature by following the fate of live HeLa cells after RNAi-mediated depletion of 120 proteins previously implicated in chromosome alignment. Surprisingly, in all cases, cells frequently entered anaphase after a delay with chronically misaligned chromosomes. Using depletion of key proteins as prototypes for defective chromosome alignment, we show that chronically misaligned chromosomes often satisfy the SAC and directly missegregate. In-depth analysis of specific molecular perturbations that prevent proper kinetochore-microtubule attachments revealed that chronically misaligned chromosomes that missegregate frequently result in micronuclei. Higher-resolution live-cell imaging indicated that, contrary to most anaphase lagging chromosomes that correct and reintegrate the main nuclei, chronically misaligned chromosomes are a strong predictor of micronuclei formation in a cancer cell model of chromosomal instability, but not in normal near-diploid cells. We provide evidence supporting that intrinsic differences in kinetochore-microtubule attachment stability on misaligned chromosomes account for this distinct outcome. Thus, chronically misaligned chromosomes that satisfy the SAC may represent a previously overlooked mechanism driving chromosomal/genomic instability during cancer cell division, and we unveil genetic conditions predisposing for these events." @default.
- W4286500378 created "2022-07-22" @default.
- W4286500378 creator A5016676719 @default.
- W4286500378 creator A5022892366 @default.
- W4286500378 creator A5040568994 @default.
- W4286500378 creator A5074171647 @default.
- W4286500378 creator A5076009876 @default.
- W4286500378 creator A5079633840 @default.
- W4286500378 date "2022-07-21" @default.
- W4286500378 modified "2023-10-16" @default.
- W4286500378 title "Misaligned chromosomes that satisfy the spindle assembly checkpoint are a strong predictor of micronuclei formation in dividing cancer cells" @default.
- W4286500378 cites W1483395792 @default.
- W4286500378 cites W1522090640 @default.
- W4286500378 cites W1601755300 @default.
- W4286500378 cites W1657157395 @default.
- W4286500378 cites W1733295177 @default.
- W4286500378 cites W1967696278 @default.
- W4286500378 cites W1974319312 @default.
- W4286500378 cites W1982341304 @default.
- W4286500378 cites W1987824985 @default.
- W4286500378 cites W2004616980 @default.
- W4286500378 cites W2005036868 @default.
- W4286500378 cites W2006124751 @default.
- W4286500378 cites W2014436930 @default.
- W4286500378 cites W2022565225 @default.
- W4286500378 cites W2026725038 @default.
- W4286500378 cites W2032542204 @default.
- W4286500378 cites W2034086671 @default.
- W4286500378 cites W2036530157 @default.
- W4286500378 cites W2037404897 @default.
- W4286500378 cites W2037685033 @default.
- W4286500378 cites W2042107535 @default.
- W4286500378 cites W2043477237 @default.
- W4286500378 cites W2045539221 @default.
- W4286500378 cites W2047521557 @default.
- W4286500378 cites W2050953095 @default.
- W4286500378 cites W2054929208 @default.
- W4286500378 cites W2057286714 @default.
- W4286500378 cites W2058703970 @default.
- W4286500378 cites W2060839127 @default.
- W4286500378 cites W2066275355 @default.
- W4286500378 cites W2067499825 @default.
- W4286500378 cites W2071174853 @default.
- W4286500378 cites W2088006036 @default.
- W4286500378 cites W2088492866 @default.
- W4286500378 cites W2095617425 @default.
- W4286500378 cites W2098318897 @default.
- W4286500378 cites W2100813299 @default.
- W4286500378 cites W2102502996 @default.
- W4286500378 cites W2102922697 @default.
- W4286500378 cites W2102960633 @default.
- W4286500378 cites W2105557635 @default.
- W4286500378 cites W2109948920 @default.
- W4286500378 cites W2117015042 @default.
- W4286500378 cites W2118562565 @default.
- W4286500378 cites W2125240364 @default.
- W4286500378 cites W2125306062 @default.
- W4286500378 cites W2126441143 @default.
- W4286500378 cites W2126499761 @default.
- W4286500378 cites W2126683450 @default.
- W4286500378 cites W2129153399 @default.
- W4286500378 cites W2137429755 @default.
- W4286500378 cites W2140493627 @default.
- W4286500378 cites W2141144464 @default.
- W4286500378 cites W2141793615 @default.
- W4286500378 cites W2144259787 @default.
- W4286500378 cites W2144412383 @default.
- W4286500378 cites W2152277325 @default.
- W4286500378 cites W2154603906 @default.
- W4286500378 cites W2156035827 @default.
- W4286500378 cites W2156783837 @default.
- W4286500378 cites W2160508741 @default.
- W4286500378 cites W2170748583 @default.
- W4286500378 cites W2317194968 @default.
- W4286500378 cites W2461106335 @default.
- W4286500378 cites W2564335181 @default.
- W4286500378 cites W2588416196 @default.
- W4286500378 cites W263277881 @default.
- W4286500378 cites W2883183618 @default.
- W4286500378 cites W2886679589 @default.
- W4286500378 cites W2891405227 @default.
- W4286500378 cites W2913794189 @default.
- W4286500378 cites W2915000951 @default.
- W4286500378 cites W2921140401 @default.
- W4286500378 cites W2967107299 @default.
- W4286500378 cites W2981751153 @default.
- W4286500378 cites W3016718031 @default.
- W4286500378 cites W3116548071 @default.
- W4286500378 cites W3130960912 @default.
- W4286500378 cites W3147598242 @default.
- W4286500378 cites W3158685382 @default.
- W4286500378 cites W3176219224 @default.
- W4286500378 cites W3178769426 @default.
- W4286500378 cites W3200400740 @default.
- W4286500378 cites W3211459544 @default.
- W4286500378 doi "https://doi.org/10.1101/2022.07.20.500828" @default.
- W4286500378 hasPublicationYear "2022" @default.
- W4286500378 type Work @default.
- W4286500378 citedByCount "1" @default.
- W4286500378 countsByYear W42865003782022 @default.