FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286511254> ?p ?o ?g. }

• W4286511254 endingPage "e12755" @default.
• W4286511254 startingPage "e12755" @default.
• W4286511254 abstract "BackgroundPlatelet‐fibrin clot contraction is critical for wound closure and maintenance of vessel patency, yet a molecular understanding of the process has lagged because of a lack of flexible quantitative assay systems capable of assaying multiple samples simultaneously.ObjectivesWe devised a sensitive and inexpensive method to assess clot contraction kinetics under multiple conditions.MethodsClot contraction was measured using time‐lapse digital photography, automated image processing with customized software, and detailed kinetic analysis using available commercial programs.ResultsOur system was responsive to alterations in platelet counts and calcium, fibrinogen, and thrombin concentrations, and our analysis detected and defined three phases of platelet‐fibrin clot formation: initiation, contraction, and stabilization. Lag time, average contraction velocity, contraction extent, and area under the curve were readily calculated from the data. Using pharmacological agents (blebbistatin and eptifibatide), we confirmed the importance of myosin IIA and the interactions of integrin αIIbβ3‐fibrinogen/fibrin in clot contraction. As further proof of our system's utility, we showed how 2‐deoxyglucose affects contraction, demonstrating the importance of platelet bioenergetics, specifically glycolysis.ConclusionsOur system is an adaptable platform for assessing the effects of multiple conditions and interventions on clot contraction kinetics in a regular laboratory setting, using readily available materials. The automated image processing software we developed will be made freely available for noncommercial uses. This assay system can be used to directly compare and define the effects of different treatments or genetic manipulations on platelet function and should provide a robust tool for future hemostasis/thrombosis research and therapeutic development." @default.
• W4286511254 created "2022-07-22" @default.
• W4286511254 creator A5000261720 @default.
• W4286511254 creator A5007472725 @default.
• W4286511254 creator A5070554662 @default.
• W4286511254 creator A5074630977 @default.
• W4286511254 creator A5085893830 @default.
• W4286511254 creator A5026838675 @default.
• W4286511254 date "2022-07-01" @default.
• W4286511254 modified "2023-10-14" @default.
• W4286511254 title "A sensitive and adaptable method to measure platelet‐fibrin clot contraction kinetics" @default.
• W4286511254 cites W1912550709 @default.
• W4286511254 cites W1967238923 @default.
• W4286511254 cites W1981248837 @default.
• W4286511254 cites W2006841845 @default.
• W4286511254 cites W2009582775 @default.
• W4286511254 cites W2010146256 @default.
• W4286511254 cites W2011193686 @default.
• W4286511254 cites W2030806904 @default.
• W4286511254 cites W2054164838 @default.
• W4286511254 cites W2056612835 @default.
• W4286511254 cites W2057637682 @default.
• W4286511254 cites W2088399299 @default.
• W4286511254 cites W2092752244 @default.
• W4286511254 cites W2097503680 @default.
• W4286511254 cites W2128913650 @default.
• W4286511254 cites W2172079983 @default.
• W4286511254 cites W2257788424 @default.
• W4286511254 cites W2345636639 @default.
• W4286511254 cites W2409882338 @default.
• W4286511254 cites W2553332304 @default.
• W4286511254 cites W2557812157 @default.
• W4286511254 cites W2572149156 @default.
• W4286511254 cites W2593092312 @default.
• W4286511254 cites W2733120897 @default.
• W4286511254 cites W2736776512 @default.
• W4286511254 cites W2744761285 @default.
• W4286511254 cites W2765204564 @default.
• W4286511254 cites W2766226726 @default.
• W4286511254 cites W2782020047 @default.
• W4286511254 cites W2903727129 @default.
• W4286511254 cites W2923030606 @default.
• W4286511254 cites W2968422623 @default.
• W4286511254 cites W2997406734 @default.
• W4286511254 cites W3093913800 @default.
• W4286511254 cites W3111499168 @default.
• W4286511254 cites W4210564819 @default.
• W4286511254 cites W4241102995 @default.
• W4286511254 doi "https://doi.org/10.1002/rth2.12755" @default.
• W4286511254 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35873218" @default.
• W4286511254 hasPublicationYear "2022" @default.
• W4286511254 type Work @default.
• W4286511254 citedByCount "2" @default.
• W4286511254 countsByYear W42865112542023 @default.
• W4286511254 crossrefType "journal-article" @default.
• W4286511254 hasAuthorship W4286511254A5000261720 @default.
• W4286511254 hasAuthorship W4286511254A5007472725 @default.
• W4286511254 hasAuthorship W4286511254A5026838675 @default.
• W4286511254 hasAuthorship W4286511254A5070554662 @default.
• W4286511254 hasAuthorship W4286511254A5074630977 @default.
• W4286511254 hasAuthorship W4286511254A5085893830 @default.
• W4286511254 hasBestOaLocation W42865112541 @default.
• W4286511254 hasConcept C12554922 @default.
• W4286511254 hasConcept C126322002 @default.
• W4286511254 hasConcept C136229726 @default.
• W4286511254 hasConcept C141071460 @default.
• W4286511254 hasConcept C163415756 @default.
• W4286511254 hasConcept C185592680 @default.
• W4286511254 hasConcept C203014093 @default.
• W4286511254 hasConcept C2778589496 @default.
• W4286511254 hasConcept C54173615 @default.
• W4286511254 hasConcept C71924100 @default.
• W4286511254 hasConcept C86803240 @default.
• W4286511254 hasConcept C89560881 @default.
• W4286511254 hasConceptScore W4286511254C12554922 @default.
• W4286511254 hasConceptScore W4286511254C126322002 @default.
• W4286511254 hasConceptScore W4286511254C136229726 @default.
• W4286511254 hasConceptScore W4286511254C141071460 @default.
• W4286511254 hasConceptScore W4286511254C163415756 @default.
• W4286511254 hasConceptScore W4286511254C185592680 @default.
• W4286511254 hasConceptScore W4286511254C203014093 @default.
• W4286511254 hasConceptScore W4286511254C2778589496 @default.
• W4286511254 hasConceptScore W4286511254C54173615 @default.
• W4286511254 hasConceptScore W4286511254C71924100 @default.
• W4286511254 hasConceptScore W4286511254C86803240 @default.
• W4286511254 hasConceptScore W4286511254C89560881 @default.
• W4286511254 hasFunder F4320306127 @default.
• W4286511254 hasFunder F4320337338 @default.
• W4286511254 hasIssue "5" @default.
• W4286511254 hasLocation W42865112541 @default.
• W4286511254 hasLocation W42865112542 @default.
• W4286511254 hasLocation W42865112543 @default.
• W4286511254 hasLocation W42865112544 @default.
• W4286511254 hasOpenAccess W4286511254 @default.
• W4286511254 hasPrimaryLocation W42865112541 @default.
• W4286511254 hasRelatedWork W2008483939 @default.
• W4286511254 hasRelatedWork W2069246489 @default.
• W4286511254 hasRelatedWork W2412885513 @default.

• next