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• W4286543209 abstract "This study aimed to reveal the presence of reactive oxygen species (ROS)-induced lipid peroxidation and DNA damage in ovine pulmonary adenocarcinomas (OPA) by evaluating malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expressions by immunohistochemical and immunofluorescence methods. Lung tissue samples were collected from 26 sheep brought to the Pathology Department for routine diagnosis. Lung tissues were fixed in 10% neutral buffered formalin, following routine procedures tissues were stained with hematoxylin and eosin. Avidin-Biotin Peroxidase method was used as immunohistochemical staining. Indirect immunofluorescence method was applied to the sections. Tumoral cells showed acinar, papillary or mixed type patterns. Only 2 of 20 cases metastasized to regional lymph nodes. All OPAs were immune positive for Jaagsiekte Sheep Retrovirus Capsid Protein (JSRV CA), MDA and 8-OHdG. The control group was negative for JSRV CA, MDA and 8-OHdG expressions. Malondialdehyde and 8-OHdG immune positive cells were statistically increased in the OPA group compared to the control group. In conclusion, our results demonstrate that higher MDA and 8-OHdG expressions in sheep with OPA suggest that OPA may be closely related to lipid peroxidation and oxidative DNA damage." @default.
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• W4286543209 date "2022-01-01" @default.
• W4286543209 modified "2023-09-26" @default.
• W4286543209 title "Assessment of MDA and 8-OHdG expressions in ovine pulmonary adenocarcinomas by immunohistochemical and immunofluorescence methods" @default.
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• W4286543209 doi "https://doi.org/10.2754/avb202291030235" @default.
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