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- W4286586720 endingPage "1424" @default.
- W4286586720 startingPage "1424" @default.
- W4286586720 abstract "Oxidative stress has been causally linked to various diseases. Electron transport chain (ETC) inhibitors such as rotenone and antimycin A are frequently used in model systems to study oxidative stress. Oxidative stress that is provoked by ETC inhibitors can be visualized using the fluorogenic probe 2',7'-dichlorodihydrofluorescein-diacetate (DCFH2-DA). Non-fluorescent DCFH2-DA crosses the plasma membrane, is deacetylated to 2',7'-dichlorodihydrofluorescein (DCFH2) by esterases, and is oxidized to its fluorescent form 2',7'-dichlorofluorescein (DCF) by intracellular ROS. DCF fluorescence can, therefore, be used as a semi-quantitative measure of general oxidative stress. However, the use of DCFH2-DA is complicated by various protocol-related factors that mediate DCFH2-to-DCF conversion independently of the degree of oxidative stress. This study therefore analyzed the influence of ancillary factors on DCF formation in the context of ETC inhibitors. It was found that ETC inhibitors trigger DCF formation in cell-free experiments when they are co-dissolved with DCFH2-DA. Moreover, the extent of DCF formation depended on the type of culture medium that was used, the pH of the assay system, the presence of fetal calf serum, and the final DCFH2-DA solvent concentration. Conclusively, experiments with DCFH2-DA should not discount the influence of protocol-related factors such as medium and mitochondrial inhibitors (and possibly other compounds) on the DCFH2-DA-DCF reaction and proper controls should always be built into the assay protocol." @default.
- W4286586720 created "2022-07-22" @default.
- W4286586720 creator A5008937484 @default.
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- W4286586720 creator A5078824354 @default.
- W4286586720 date "2022-07-22" @default.
- W4286586720 modified "2023-10-12" @default.
- W4286586720 title "Experimental Conditions That Influence the Utility of 2′7′-Dichlorodihydrofluorescein Diacetate (DCFH2-DA) as a Fluorogenic Biosensor for Mitochondrial Redox Status" @default.
- W4286586720 cites W1122380940 @default.
- W4286586720 cites W1546618745 @default.
- W4286586720 cites W1554017667 @default.
- W4286586720 cites W1567637492 @default.
- W4286586720 cites W1576822037 @default.
- W4286586720 cites W1855629639 @default.
- W4286586720 cites W1925799544 @default.
- W4286586720 cites W1944769381 @default.
- W4286586720 cites W1967853821 @default.
- W4286586720 cites W1969367445 @default.
- W4286586720 cites W1972779889 @default.
- W4286586720 cites W1973094580 @default.
- W4286586720 cites W1976392585 @default.
- W4286586720 cites W1979731389 @default.
- W4286586720 cites W1981120411 @default.
- W4286586720 cites W1986956461 @default.
- W4286586720 cites W1988521879 @default.
- W4286586720 cites W1988666366 @default.
- W4286586720 cites W1989114406 @default.
- W4286586720 cites W1989729441 @default.
- W4286586720 cites W1990893386 @default.
- W4286586720 cites W1991881222 @default.
- W4286586720 cites W1991952977 @default.
- W4286586720 cites W1997684038 @default.
- W4286586720 cites W1997757821 @default.
- W4286586720 cites W2001355420 @default.
- W4286586720 cites W2003171741 @default.
- W4286586720 cites W2003399049 @default.
- W4286586720 cites W2004691367 @default.
- W4286586720 cites W2005374900 @default.
- W4286586720 cites W2006012100 @default.
- W4286586720 cites W2012854362 @default.
- W4286586720 cites W2013103361 @default.
- W4286586720 cites W2014600874 @default.
- W4286586720 cites W2018039789 @default.
- W4286586720 cites W2020138346 @default.
- W4286586720 cites W2026525843 @default.
- W4286586720 cites W2028520206 @default.
- W4286586720 cites W2030010285 @default.
- W4286586720 cites W2030363360 @default.
- W4286586720 cites W2031544327 @default.
- W4286586720 cites W2035689722 @default.
- W4286586720 cites W2035705887 @default.
- W4286586720 cites W2035845912 @default.
- W4286586720 cites W2036019407 @default.
- W4286586720 cites W2036127346 @default.
- W4286586720 cites W2036167505 @default.
- W4286586720 cites W2037857730 @default.
- W4286586720 cites W2037912573 @default.
- W4286586720 cites W2047758994 @default.
- W4286586720 cites W2050043127 @default.
- W4286586720 cites W2050897300 @default.
- W4286586720 cites W2054226518 @default.
- W4286586720 cites W2057810394 @default.
- W4286586720 cites W2059503903 @default.
- W4286586720 cites W2060363769 @default.
- W4286586720 cites W2065263524 @default.
- W4286586720 cites W2068630823 @default.
- W4286586720 cites W2070780704 @default.
- W4286586720 cites W2072813591 @default.
- W4286586720 cites W2074210465 @default.
- W4286586720 cites W2080304693 @default.
- W4286586720 cites W2081433315 @default.
- W4286586720 cites W2082970352 @default.
- W4286586720 cites W2089032804 @default.
- W4286586720 cites W2096386031 @default.
- W4286586720 cites W2097259163 @default.
- W4286586720 cites W2098227836 @default.
- W4286586720 cites W2101528448 @default.
- W4286586720 cites W2101861123 @default.
- W4286586720 cites W2103858492 @default.
- W4286586720 cites W2111229603 @default.
- W4286586720 cites W2113651620 @default.
- W4286586720 cites W2114467922 @default.
- W4286586720 cites W2122091775 @default.
- W4286586720 cites W2122341467 @default.
- W4286586720 cites W2126516300 @default.
- W4286586720 cites W2136167073 @default.
- W4286586720 cites W2141137988 @default.
- W4286586720 cites W2147465625 @default.
- W4286586720 cites W2147564573 @default.
- W4286586720 cites W2147612602 @default.
- W4286586720 cites W2147993424 @default.
- W4286586720 cites W2148390704 @default.