Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286587462> ?p ?o ?g. }
- W4286587462 abstract "We report the cloning of a 12-year-old transgenic green fluorescent protein (GFP) monkey by somatic cell nuclear transfer (SCNT) and base editing of the embryos, accompanied with safety evaluation of adenine base editors (ABEs). We first show the ability of ABEmax to silence GFP through A-to-G editing of the GFP sequence in 293T cells. Subsequently, using donor cells from a monkey expressing GFP, we have successfully generated 207 ABEmax-edited (SCNT-ABE) and 87 wild-type (SCNT) embryos for embryo transfer, genotyping, and genome and transcriptome analysis. SCNT-ABE and SCNT embryos are compared for off-target analysis without the interference of genetic variants using a new method named as OA-SCNT. ABEmax does not induce obvious off-target DNA mutations but induces widespread off-target RNA mutations, 35% of which are exonic, in edited monkey embryos. These results provide important references for clinical application of ABE." @default.
- W4286587462 created "2022-07-22" @default.
- W4286587462 creator A5029491236 @default.
- W4286587462 creator A5033185368 @default.
- W4286587462 creator A5046463041 @default.
- W4286587462 creator A5046819993 @default.
- W4286587462 creator A5050334079 @default.
- W4286587462 creator A5053701912 @default.
- W4286587462 creator A5057787567 @default.
- W4286587462 creator A5060599889 @default.
- W4286587462 creator A5071145977 @default.
- W4286587462 creator A5076999427 @default.
- W4286587462 creator A5085762347 @default.
- W4286587462 creator A5088797126 @default.
- W4286587462 creator A5091129802 @default.
- W4286587462 date "2022-07-22" @default.
- W4286587462 modified "2023-09-27" @default.
- W4286587462 title "Cloning and base editing of GFP transgenic rhesus monkey and off-target analysis" @default.
- W4286587462 cites W1482671240 @default.
- W4286587462 cites W1964807436 @default.
- W4286587462 cites W1983005863 @default.
- W4286587462 cites W1984068087 @default.
- W4286587462 cites W1999453796 @default.
- W4286587462 cites W2010765881 @default.
- W4286587462 cites W2035618305 @default.
- W4286587462 cites W2060965924 @default.
- W4286587462 cites W2103441770 @default.
- W4286587462 cites W2108234281 @default.
- W4286587462 cites W2119180969 @default.
- W4286587462 cites W2128505445 @default.
- W4286587462 cites W2138207763 @default.
- W4286587462 cites W2146005104 @default.
- W4286587462 cites W2336346625 @default.
- W4286587462 cites W2336828812 @default.
- W4286587462 cites W2337209831 @default.
- W4286587462 cites W2607273379 @default.
- W4286587462 cites W2626796823 @default.
- W4286587462 cites W2737196618 @default.
- W4286587462 cites W2766599608 @default.
- W4286587462 cites W2784769965 @default.
- W4286587462 cites W2803557679 @default.
- W4286587462 cites W2805505209 @default.
- W4286587462 cites W2867868648 @default.
- W4286587462 cites W2889368001 @default.
- W4286587462 cites W2897559392 @default.
- W4286587462 cites W2916364858 @default.
- W4286587462 cites W2917855738 @default.
- W4286587462 cites W2939749480 @default.
- W4286587462 cites W2943918361 @default.
- W4286587462 cites W2951233129 @default.
- W4286587462 cites W2951912016 @default.
- W4286587462 cites W2973637541 @default.
- W4286587462 cites W2993258524 @default.
- W4286587462 cites W3119813698 @default.
- W4286587462 cites W3129026263 @default.
- W4286587462 cites W3161425918 @default.
- W4286587462 cites W3163681195 @default.
- W4286587462 doi "https://doi.org/10.1126/sciadv.abo3123" @default.
- W4286587462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35867792" @default.
- W4286587462 hasPublicationYear "2022" @default.
- W4286587462 type Work @default.
- W4286587462 citedByCount "1" @default.
- W4286587462 countsByYear W42865874622023 @default.
- W4286587462 crossrefType "journal-article" @default.
- W4286587462 hasAuthorship W4286587462A5029491236 @default.
- W4286587462 hasAuthorship W4286587462A5033185368 @default.
- W4286587462 hasAuthorship W4286587462A5046463041 @default.
- W4286587462 hasAuthorship W4286587462A5046819993 @default.
- W4286587462 hasAuthorship W4286587462A5050334079 @default.
- W4286587462 hasAuthorship W4286587462A5053701912 @default.
- W4286587462 hasAuthorship W4286587462A5057787567 @default.
- W4286587462 hasAuthorship W4286587462A5060599889 @default.
- W4286587462 hasAuthorship W4286587462A5071145977 @default.
- W4286587462 hasAuthorship W4286587462A5076999427 @default.
- W4286587462 hasAuthorship W4286587462A5085762347 @default.
- W4286587462 hasAuthorship W4286587462A5088797126 @default.
- W4286587462 hasAuthorship W4286587462A5091129802 @default.
- W4286587462 hasBestOaLocation W42865874622 @default.
- W4286587462 hasConcept C102230213 @default.
- W4286587462 hasConcept C104317684 @default.
- W4286587462 hasConcept C121050878 @default.
- W4286587462 hasConcept C134305767 @default.
- W4286587462 hasConcept C142613039 @default.
- W4286587462 hasConcept C150194340 @default.
- W4286587462 hasConcept C153911025 @default.
- W4286587462 hasConcept C162317418 @default.
- W4286587462 hasConcept C196843134 @default.
- W4286587462 hasConcept C199360897 @default.
- W4286587462 hasConcept C202438428 @default.
- W4286587462 hasConcept C2778177303 @default.
- W4286587462 hasConcept C41008148 @default.
- W4286587462 hasConcept C54355233 @default.
- W4286587462 hasConcept C86803240 @default.
- W4286587462 hasConcept C87073359 @default.
- W4286587462 hasConcept C95444343 @default.
- W4286587462 hasConceptScore W4286587462C102230213 @default.
- W4286587462 hasConceptScore W4286587462C104317684 @default.
- W4286587462 hasConceptScore W4286587462C121050878 @default.
- W4286587462 hasConceptScore W4286587462C134305767 @default.
- W4286587462 hasConceptScore W4286587462C142613039 @default.