Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286680891> ?p ?o ?g. }
- W4286680891 abstract "Abstract Gene expression heterogeneity is ubiquitous within single cell datasets, even among cells of the same type. Heritable expression differences, defined here as those which persist over multiple cell divisions, are of particular interest, as they can underlie processes including cell differentiation during development as well as the clonal selection of drug-resistant cancer cells. However, heritable sources of variation are difficult to disentangle from non-heritable ones, such as cell cycle stage, asynchronous transcription, and measurement noise. Since heritable states should be shared by lineally related cells, we sought to leverage CRISPR-based lineage tracing, together with single cell molecular profiling, to discriminate between heritable and non-heritable variation in gene expression. We show that high efficiency capture of lineage profiles alongside single cell gene expression enables accurate lineage tree reconstruction and reveals an abundance of progressive, heritable gene expression changes. We find that a subset of these are likely mediated by structural genetic variation (copy number alterations, translocations), but that the stable attributes of others cannot be understood with expression data alone. Towards addressing this, we develop a method to capture cell lineage histories alongside single cell chromatin accessibility profiles, such that expression and chromatin accessibility of closely related cells can be linked via their lineage histories. We call this indirect “coassay” approach “THE LORAX” and leverage it to explore the genetic and epigenetic mechanisms underlying heritable gene expression changes. Using this approach, we show that we can discern between heritable gene expression differences mediated by large and small copy number changes, trans effects, and possible epigenetic variation." @default.
- W4286680891 created "2022-07-23" @default.
- W4286680891 creator A5005318156 @default.
- W4286680891 creator A5007281089 @default.
- W4286680891 creator A5022715650 @default.
- W4286680891 date "2022-05-12" @default.
- W4286680891 modified "2023-10-17" @default.
- W4286680891 title "Tethering distinct molecular profiles of single cells by their lineage histories to investigate sources of cell state heterogeneity" @default.
- W4286680891 cites W1492083415 @default.
- W4286680891 cites W1991410775 @default.
- W4286680891 cites W2027685672 @default.
- W4286680891 cites W2030017878 @default.
- W4286680891 cites W2054872934 @default.
- W4286680891 cites W2074174832 @default.
- W4286680891 cites W2097238709 @default.
- W4286680891 cites W2164740045 @default.
- W4286680891 cites W2169269276 @default.
- W4286680891 cites W2203229763 @default.
- W4286680891 cites W2406991867 @default.
- W4286680891 cites W2515000591 @default.
- W4286680891 cites W2561754210 @default.
- W4286680891 cites W2577994754 @default.
- W4286680891 cites W2747545374 @default.
- W4286680891 cites W2781886817 @default.
- W4286680891 cites W2785797026 @default.
- W4286680891 cites W2794478094 @default.
- W4286680891 cites W2795331645 @default.
- W4286680891 cites W2796538159 @default.
- W4286680891 cites W2802302382 @default.
- W4286680891 cites W2868208638 @default.
- W4286680891 cites W2885735662 @default.
- W4286680891 cites W2886855629 @default.
- W4286680891 cites W2887910040 @default.
- W4286680891 cites W2889236955 @default.
- W4286680891 cites W2896203075 @default.
- W4286680891 cites W2902451951 @default.
- W4286680891 cites W2907783748 @default.
- W4286680891 cites W2916020270 @default.
- W4286680891 cites W2920802237 @default.
- W4286680891 cites W2946902586 @default.
- W4286680891 cites W2949177718 @default.
- W4286680891 cites W2949307008 @default.
- W4286680891 cites W2949490462 @default.
- W4286680891 cites W2950195808 @default.
- W4286680891 cites W2950491429 @default.
- W4286680891 cites W2951343553 @default.
- W4286680891 cites W2952814943 @default.
- W4286680891 cites W2970098032 @default.
- W4286680891 cites W2979911343 @default.
- W4286680891 cites W2985765936 @default.
- W4286680891 cites W3002219094 @default.
- W4286680891 cites W3005287223 @default.
- W4286680891 cites W3005469082 @default.
- W4286680891 cites W3013268105 @default.
- W4286680891 cites W3013900499 @default.
- W4286680891 cites W3015748341 @default.
- W4286680891 cites W3024554698 @default.
- W4286680891 cites W3026159272 @default.
- W4286680891 cites W3033278867 @default.
- W4286680891 cites W3033689934 @default.
- W4286680891 cites W3044262883 @default.
- W4286680891 cites W3045872583 @default.
- W4286680891 cites W3081575535 @default.
- W4286680891 cites W3093858607 @default.
- W4286680891 cites W3120612913 @default.
- W4286680891 cites W3122943587 @default.
- W4286680891 cites W3131634425 @default.
- W4286680891 cites W3138433830 @default.
- W4286680891 cites W3155656876 @default.
- W4286680891 cites W3161116336 @default.
- W4286680891 cites W3171133702 @default.
- W4286680891 cites W3185053431 @default.
- W4286680891 cites W3197625584 @default.
- W4286680891 cites W3201343366 @default.
- W4286680891 cites W3211738199 @default.
- W4286680891 cites W4200187755 @default.
- W4286680891 cites W4200227839 @default.
- W4286680891 cites W4206964309 @default.
- W4286680891 cites W4210519974 @default.
- W4286680891 cites W4213319096 @default.
- W4286680891 doi "https://doi.org/10.1101/2022.05.12.491602" @default.
- W4286680891 hasPublicationYear "2022" @default.
- W4286680891 type Work @default.
- W4286680891 citedByCount "3" @default.
- W4286680891 countsByYear W42866808912022 @default.
- W4286680891 countsByYear W42866808912023 @default.
- W4286680891 crossrefType "posted-content" @default.
- W4286680891 hasAuthorship W4286680891A5005318156 @default.
- W4286680891 hasAuthorship W4286680891A5007281089 @default.
- W4286680891 hasAuthorship W4286680891A5022715650 @default.
- W4286680891 hasBestOaLocation W42866808911 @default.
- W4286680891 hasConcept C104317684 @default.
- W4286680891 hasConcept C148738053 @default.
- W4286680891 hasConcept C1491633281 @default.
- W4286680891 hasConcept C150194340 @default.
- W4286680891 hasConcept C165864922 @default.
- W4286680891 hasConcept C18431079 @default.
- W4286680891 hasConcept C189014844 @default.
- W4286680891 hasConcept C2776817793 @default.
- W4286680891 hasConcept C2776950831 @default.