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- W4286716098 abstract "Abstract Background Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have emerged as a powerful tool for disease modeling, though their immature nature currently limits translation into clinical practice. Maturation strategies increasingly pay attention to cardiac metabolism because of its pivotal role in cardiomyocyte development and function. Moreover, aberrances in cardiac metabolism are central to the pathogenesis of cardiac disease. Thus, proper modeling of human cardiac disease warrants careful characterization of the metabolic properties of iPSC-CMs. Methods Here, we examined the effect of maturation protocols on healthy iPSC-CMs applied in 23 studies and compared fold changes in functional metabolic characteristics to assess the level of maturation. In addition, pathological metabolic remodeling was assessed in 13 iPSC-CM studies that focus on hypertrophic cardiomyopathy (HCM), which is characterized by abnormalities in metabolism. Results Matured iPSC-CMs were characterized by mitochondrial maturation, increased oxidative capacity and enhanced fatty acid use for energy production. HCM iPSC-CMs presented varying degrees of metabolic remodeling ranging from compensatory to energy depletion stages, likely due to the different types of mutations and clinical phenotypes modeled. HCM further displayed early onset hypertrophy, independent of the type of mutation or disease stage. Conclusions Maturation strategies improve the metabolic characteristics of iPSC-CMs, but not to the level of the adult heart. Therefore, a combination of maturation strategies might prove to be more effective. Due to early onset hypertrophy, HCM iPSC-CMs may be less suitable to detect early disease modifiers in HCM and might prove more useful to examine the effects of gene editing and new drugs in advanced disease stages. With this review, we provide an overview of the assays used for characterization of cardiac metabolism in iPSC-CMs and advise on which metabolic assays to include in future maturation and disease modeling studies." @default.
- W4286716098 created "2022-07-23" @default.
- W4286716098 creator A5004120429 @default.
- W4286716098 creator A5008468085 @default.
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- W4286716098 creator A5064317519 @default.
- W4286716098 creator A5089008867 @default.
- W4286716098 date "2022-07-23" @default.
- W4286716098 modified "2023-10-14" @default.
- W4286716098 title "Characterization of cardiac metabolism in iPSC-derived cardiomyocytes: lessons from maturation and disease modeling" @default.
- W4286716098 cites W1606922151 @default.
- W4286716098 cites W1837909929 @default.
- W4286716098 cites W1938218641 @default.
- W4286716098 cites W1965392584 @default.
- W4286716098 cites W1966187802 @default.
- W4286716098 cites W1968550558 @default.
- W4286716098 cites W1993431987 @default.
- W4286716098 cites W2016752484 @default.
- W4286716098 cites W2022229270 @default.
- W4286716098 cites W2033765226 @default.
- W4286716098 cites W2042091538 @default.
- W4286716098 cites W2050824263 @default.
- W4286716098 cites W2071518542 @default.
- W4286716098 cites W2079592630 @default.
- W4286716098 cites W2082434654 @default.
- W4286716098 cites W2096579994 @default.
- W4286716098 cites W2098188264 @default.
- W4286716098 cites W2128653444 @default.
- W4286716098 cites W2138203975 @default.
- W4286716098 cites W2139200038 @default.
- W4286716098 cites W2141763505 @default.
- W4286716098 cites W2143505819 @default.
- W4286716098 cites W2159697997 @default.
- W4286716098 cites W2165564780 @default.
- W4286716098 cites W2172517621 @default.
- W4286716098 cites W2266308521 @default.
- W4286716098 cites W2347150262 @default.
- W4286716098 cites W2563313653 @default.
- W4286716098 cites W2565014548 @default.
- W4286716098 cites W2571511532 @default.
- W4286716098 cites W2611938973 @default.
- W4286716098 cites W2614974118 @default.
- W4286716098 cites W2616302857 @default.
- W4286716098 cites W2623013881 @default.
- W4286716098 cites W2729548803 @default.
- W4286716098 cites W2743978568 @default.
- W4286716098 cites W2754548624 @default.
- W4286716098 cites W2756221746 @default.
- W4286716098 cites W2763140785 @default.
- W4286716098 cites W2764024258 @default.
- W4286716098 cites W2765646903 @default.
- W4286716098 cites W2768142457 @default.
- W4286716098 cites W2770578593 @default.
- W4286716098 cites W2786635794 @default.
- W4286716098 cites W2787032606 @default.
- W4286716098 cites W2789409476 @default.
- W4286716098 cites W2793820120 @default.
- W4286716098 cites W2793879355 @default.
- W4286716098 cites W2794616878 @default.
- W4286716098 cites W2800658350 @default.
- W4286716098 cites W2800692959 @default.
- W4286716098 cites W2802543878 @default.
- W4286716098 cites W2809116340 @default.
- W4286716098 cites W2810747712 @default.
- W4286716098 cites W2886927628 @default.
- W4286716098 cites W2888495066 @default.
- W4286716098 cites W2893581659 @default.
- W4286716098 cites W2896255493 @default.
- W4286716098 cites W2897602275 @default.
- W4286716098 cites W2905412801 @default.
- W4286716098 cites W2911529505 @default.
- W4286716098 cites W2934563714 @default.
- W4286716098 cites W2944179239 @default.
- W4286716098 cites W2945419690 @default.
- W4286716098 cites W2945624177 @default.
- W4286716098 cites W2953187830 @default.
- W4286716098 cites W2971619350 @default.
- W4286716098 cites W2974176177 @default.
- W4286716098 cites W2975681149 @default.
- W4286716098 cites W2988418871 @default.
- W4286716098 cites W2996314128 @default.
- W4286716098 cites W3001201582 @default.
- W4286716098 cites W3003675501 @default.
- W4286716098 cites W3004299218 @default.
- W4286716098 cites W3011609945 @default.
- W4286716098 cites W3011937803 @default.
- W4286716098 cites W3015529688 @default.
- W4286716098 cites W3027041885 @default.
- W4286716098 cites W3029730275 @default.
- W4286716098 cites W3035054819 @default.
- W4286716098 cites W3036813760 @default.
- W4286716098 cites W3037173749 @default.
- W4286716098 cites W3044901851 @default.
- W4286716098 cites W3045400892 @default.
- W4286716098 cites W3080092630 @default.
- W4286716098 cites W3092932642 @default.