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• W4286768800 abstract "Introduction: The commonly used method for hematocrit detection, by visual examination of microcapillary tube, known as micro-HCT, is subjective but still remains one of the key sources for false hematocrit evaluation. Analytical automation techniques have increased standardization of RBC indexes detection; however, indirect hematocrit measurements by blood analyzer, the automated HCT, does not correlate well with micro-HCT results in patients with hematological pathologies. We aimed to overcome those disadvantages in micro-HCT analysis by using ImageJ, processing software.&#x0D; Methods: 223 blood samples from the general population and 19 from sickle cell disease patients were examined in parallel for hematocrit values using the automated HCT, standard micro-HCT and ImageJ micro-HCT methods.&#x0D; Results: For the general population samples, the ImageJ values were significantly higher than the corresponding values evaluated by standard micro-HCT and automated HCT, except for the 0 to 2 months old newborns, in which the automated HCT results were similar to the ImageJ evaluated HCT. Similar to the general population cohort, we found significantly higher values measured by ImageJ compared to either micro-HCT or the automated HCT in SCD patients. Correspondent differences for the MCV and MCHC were also found.&#x0D; Conclusions: This study introduces micro-HCT assessment technique using the image-analysis module of ImageJ software. This procedure allows overcoming most of the data errors associated with the standard micro-HCT evaluation and can replace the use of complicated and expensive automated equipment. The presented results may be also used to develop new standards for calculations of hematocrit and associated parameters for routine clinical practice." @default.
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• W4286768800 date "2022-06-29" @default.
• W4286768800 modified "2023-09-30" @default.
• W4286768800 title "BACK TO THE “GOLD STANDARD”: HOW PRECISE IS HEMATOCRIT DETECTION TODAY?" @default.
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• W4286768800 doi "https://doi.org/10.4084/mjhid.2022.049" @default.
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