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• W4286776259 endingPage "463361" @default.
• W4286776259 startingPage "463361" @default.
• W4286776259 abstract "• Commercially available pyrene-1-boronic acid was effective to label catecholamines. • On-tissue labeling strategy was developed to label catecholamines in-situ . • The strategy succeeded in imaging catecholamines in adrenal gland tissue sections. • The strategy proved to be robust, easy-to-use and low-cost. • Distributions of catecholamines obtained by MALDI-MSI matched well optical images. In this study, an on-tissue chemical labeling - matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) method was developed for visualization of the distribution of three catecholamine (CA) compounds (dopamine, epinephrine and norepinephrine) in porcine adrenal gland. Commercially available pyrene-1-boronic acid (PBA) was employed as an effective in situ derivatizing reagent dissolved in acetonitrile containing 0.1% pyridine for the chemical labeling and the matrix coating. Without extra matrix coating, the tissue section was directly analyzed by MALDI-MS. The detection specificity and sensitivity were greatly improved with the on-tissue PBA labeling and successful imaging of the three CAs in porcine adrenal gland was achieved. Compared with previously reported methods for MALDI-MSI of the CAs, the analytical strategy proposed in the study provided a robust, easy-to-use and low-cost on-tissue chemical derivatization method that facilitated simultaneous molecular imaging of the three compounds." @default.
• W4286776259 created "2022-07-23" @default.
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• W4286776259 date "2022-08-01" @default.
• W4286776259 modified "2023-10-13" @default.
• W4286776259 title "On-tissue pyrene-1-boronic acid labeling assisted MALDI imaging of catecholamines in porcine adrenal gland" @default.
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• W4286776259 doi "https://doi.org/10.1016/j.chroma.2022.463361" @default.
• W4286776259 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35914408" @default.
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