Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286797685> ?p ?o ?g. }
- W4286797685 endingPage "8278" @default.
- W4286797685 startingPage "8262" @default.
- W4286797685 abstract "We recently reported that serine-arginine-rich (SR) protein-mediated pre-mRNA structural remodeling generates a pre-mRNA 3D structural scaffold that is stably recognized by the early spliceosomal components. However, the intermediate steps between the free pre-mRNA and the assembled early spliceosome are not yet characterized. By probing the early spliceosomal complexes in vitro and RNA-protein interactions in vivo, we show that the SR proteins bind the pre-mRNAs cooperatively generating a substrate that recruits U1 snRNP and U2AF65 in a splice signal-independent manner. Excess U1 snRNP selectively displaces some of the SR protein molecules from the pre-mRNA generating the substrate for splice signal-specific, sequential recognition by U1 snRNP, U2AF65 and U2AF35. Our work thus identifies a novel function of U1 snRNP in mammalian splicing substrate definition, explains the need for excess U1 snRNP compared to other U snRNPs in vivo, demonstrates how excess SR proteins could inhibit splicing, and provides a conceptual basis to examine if this mechanism of splicing substrate definition is employed by other splicing regulatory proteins." @default.
- W4286797685 created "2022-07-24" @default.
- W4286797685 creator A5034258593 @default.
- W4286797685 creator A5067800581 @default.
- W4286797685 date "2022-07-25" @default.
- W4286797685 modified "2023-10-14" @default.
- W4286797685 title "Cooperative engagement and subsequent selective displacement of SR proteins define the pre-mRNA 3D structural scaffold for early spliceosome assembly" @default.
- W4286797685 cites W1685935480 @default.
- W4286797685 cites W1811005029 @default.
- W4286797685 cites W1915661113 @default.
- W4286797685 cites W1965544475 @default.
- W4286797685 cites W1970108031 @default.
- W4286797685 cites W1993816390 @default.
- W4286797685 cites W2005844899 @default.
- W4286797685 cites W2012098861 @default.
- W4286797685 cites W2026601158 @default.
- W4286797685 cites W2029979788 @default.
- W4286797685 cites W2034793790 @default.
- W4286797685 cites W2053998703 @default.
- W4286797685 cites W2054372604 @default.
- W4286797685 cites W2057368600 @default.
- W4286797685 cites W2073954801 @default.
- W4286797685 cites W2081132215 @default.
- W4286797685 cites W2082138422 @default.
- W4286797685 cites W2082337556 @default.
- W4286797685 cites W2085978470 @default.
- W4286797685 cites W2093485679 @default.
- W4286797685 cites W2103999254 @default.
- W4286797685 cites W2105575347 @default.
- W4286797685 cites W2110163439 @default.
- W4286797685 cites W2116911290 @default.
- W4286797685 cites W2119137031 @default.
- W4286797685 cites W2133528590 @default.
- W4286797685 cites W2135924983 @default.
- W4286797685 cites W2146219830 @default.
- W4286797685 cites W2148393113 @default.
- W4286797685 cites W2151548440 @default.
- W4286797685 cites W2154247868 @default.
- W4286797685 cites W2156186077 @default.
- W4286797685 cites W2161586678 @default.
- W4286797685 cites W2167279371 @default.
- W4286797685 cites W2204166508 @default.
- W4286797685 cites W2307041907 @default.
- W4286797685 cites W2399067949 @default.
- W4286797685 cites W2577586309 @default.
- W4286797685 cites W2590568317 @default.
- W4286797685 cites W2600528227 @default.
- W4286797685 cites W2767345597 @default.
- W4286797685 cites W2774429523 @default.
- W4286797685 cites W2784270681 @default.
- W4286797685 cites W2785743033 @default.
- W4286797685 cites W2806026562 @default.
- W4286797685 cites W2971559235 @default.
- W4286797685 cites W2980949872 @default.
- W4286797685 cites W3024286496 @default.
- W4286797685 cites W3093432916 @default.
- W4286797685 cites W3131196013 @default.
- W4286797685 cites W3137008460 @default.
- W4286797685 cites W3140156444 @default.
- W4286797685 cites W3176730636 @default.
- W4286797685 cites W3214218534 @default.
- W4286797685 cites W41779663 @default.
- W4286797685 cites W4234676107 @default.
- W4286797685 cites W48376435 @default.
- W4286797685 doi "https://doi.org/10.1093/nar/gkac636" @default.
- W4286797685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35871302" @default.
- W4286797685 hasPublicationYear "2022" @default.
- W4286797685 type Work @default.
- W4286797685 citedByCount "2" @default.
- W4286797685 countsByYear W42867976852023 @default.
- W4286797685 crossrefType "journal-article" @default.
- W4286797685 hasAuthorship W4286797685A5034258593 @default.
- W4286797685 hasAuthorship W4286797685A5067800581 @default.
- W4286797685 hasBestOaLocation W42867976851 @default.
- W4286797685 hasConcept C102063271 @default.
- W4286797685 hasConcept C104317684 @default.
- W4286797685 hasConcept C10447831 @default.
- W4286797685 hasConcept C105580179 @default.
- W4286797685 hasConcept C40000846 @default.
- W4286797685 hasConcept C41282012 @default.
- W4286797685 hasConcept C53645450 @default.
- W4286797685 hasConcept C54355233 @default.
- W4286797685 hasConcept C54458228 @default.
- W4286797685 hasConcept C62478195 @default.
- W4286797685 hasConcept C67705224 @default.
- W4286797685 hasConcept C68483431 @default.
- W4286797685 hasConcept C72994624 @default.
- W4286797685 hasConcept C75934600 @default.
- W4286797685 hasConcept C86803240 @default.
- W4286797685 hasConcept C94671646 @default.
- W4286797685 hasConcept C95444343 @default.
- W4286797685 hasConceptScore W4286797685C102063271 @default.
- W4286797685 hasConceptScore W4286797685C104317684 @default.
- W4286797685 hasConceptScore W4286797685C10447831 @default.
- W4286797685 hasConceptScore W4286797685C105580179 @default.
- W4286797685 hasConceptScore W4286797685C40000846 @default.
- W4286797685 hasConceptScore W4286797685C41282012 @default.
- W4286797685 hasConceptScore W4286797685C53645450 @default.