Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286852012> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W4286852012 abstract "We report a novel adaptation of the Radial Monolayer Cell Migration assay, first reported to measure the radial migration of adherent tumor cells on extracellular matrix proteins, for measuring the motility of fluorescently-labeled, non-adherent human or murine effector immune cells. This technique employs a stainless steel manifold and 10-well Teflon slide to focally deposit non-adherent T cells into wells prepared with either confluent tumor cell monolayers or extracellular matrix proteins. Light and/or multi-channel fluorescence microscopy is used to track the movement and behavior of the effector cells over time. Fluorescent dyes and/or viral vectors that code for fluorescent transgenes are used to differentially label the cell types for imaging. This method is distinct from similar-type in vitro assays that track horizontal or vertical migration/invasion utilizing slide chambers, agar or transwell plates. The assay allows detailed imaging data to be collected with different cell types distinguished by specific fluorescent markers; even specific subpopulations of cells (i.e., transduced/nontransduced) can be monitored. Surface intensity fluorescence plots are generated using specific fluorescence channels that correspond to the migrating cell type. This allows for better visualization of the non-adherent immune cell mobility at specific times. It is possible to gather evidence of other effector cell functions, such as cytotoxicity or transfer of viral vectors from effector to target cells, as well. Thus, the method allows researchers to microscopically document cell-to-cell interactions of differentially-labeled, non-adherent with adherent cells of various types. Such information may be especially relevant in the assessment of biologically-manipulated or activated immune cell types, where visual proof of functionality is desired with tumor target cells before their use for cancer therapy." @default.
- W4286852012 created "2022-07-25" @default.
- W4286852012 creator A5010449744 @default.
- W4286852012 creator A5015193605 @default.
- W4286852012 creator A5026711791 @default.
- W4286852012 creator A5030901529 @default.
- W4286852012 creator A5043863125 @default.
- W4286852012 creator A5063562007 @default.
- W4286852012 creator A5079188247 @default.
- W4286852012 creator A5079316205 @default.
- W4286852012 creator A5083605239 @default.
- W4286852012 date "2015-02-11" @default.
- W4286852012 modified "2023-10-15" @default.
- W4286852012 title "Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes" @default.
- W4286852012 doi "https://doi.org/10.3791/52416-v" @default.
- W4286852012 hasPublicationYear "2015" @default.
- W4286852012 type Work @default.
- W4286852012 citedByCount "0" @default.
- W4286852012 crossrefType "journal-article" @default.
- W4286852012 hasAuthorship W4286852012A5010449744 @default.
- W4286852012 hasAuthorship W4286852012A5015193605 @default.
- W4286852012 hasAuthorship W4286852012A5026711791 @default.
- W4286852012 hasAuthorship W4286852012A5030901529 @default.
- W4286852012 hasAuthorship W4286852012A5043863125 @default.
- W4286852012 hasAuthorship W4286852012A5063562007 @default.
- W4286852012 hasAuthorship W4286852012A5079188247 @default.
- W4286852012 hasAuthorship W4286852012A5079316205 @default.
- W4286852012 hasAuthorship W4286852012A5083605239 @default.
- W4286852012 hasBestOaLocation W42868520122 @default.
- W4286852012 hasConcept C121332964 @default.
- W4286852012 hasConcept C12554922 @default.
- W4286852012 hasConcept C137738243 @default.
- W4286852012 hasConcept C1491633281 @default.
- W4286852012 hasConcept C153911025 @default.
- W4286852012 hasConcept C154317977 @default.
- W4286852012 hasConcept C169274487 @default.
- W4286852012 hasConcept C185592680 @default.
- W4286852012 hasConcept C189014844 @default.
- W4286852012 hasConcept C189165786 @default.
- W4286852012 hasConcept C202751555 @default.
- W4286852012 hasConcept C2779178360 @default.
- W4286852012 hasConcept C28406088 @default.
- W4286852012 hasConcept C51785407 @default.
- W4286852012 hasConcept C55493867 @default.
- W4286852012 hasConcept C62520636 @default.
- W4286852012 hasConcept C86803240 @default.
- W4286852012 hasConcept C91881484 @default.
- W4286852012 hasConcept C95444343 @default.
- W4286852012 hasConceptScore W4286852012C121332964 @default.
- W4286852012 hasConceptScore W4286852012C12554922 @default.
- W4286852012 hasConceptScore W4286852012C137738243 @default.
- W4286852012 hasConceptScore W4286852012C1491633281 @default.
- W4286852012 hasConceptScore W4286852012C153911025 @default.
- W4286852012 hasConceptScore W4286852012C154317977 @default.
- W4286852012 hasConceptScore W4286852012C169274487 @default.
- W4286852012 hasConceptScore W4286852012C185592680 @default.
- W4286852012 hasConceptScore W4286852012C189014844 @default.
- W4286852012 hasConceptScore W4286852012C189165786 @default.
- W4286852012 hasConceptScore W4286852012C202751555 @default.
- W4286852012 hasConceptScore W4286852012C2779178360 @default.
- W4286852012 hasConceptScore W4286852012C28406088 @default.
- W4286852012 hasConceptScore W4286852012C51785407 @default.
- W4286852012 hasConceptScore W4286852012C55493867 @default.
- W4286852012 hasConceptScore W4286852012C62520636 @default.
- W4286852012 hasConceptScore W4286852012C86803240 @default.
- W4286852012 hasConceptScore W4286852012C91881484 @default.
- W4286852012 hasConceptScore W4286852012C95444343 @default.
- W4286852012 hasIssue "96" @default.
- W4286852012 hasLocation W42868520121 @default.
- W4286852012 hasLocation W42868520122 @default.
- W4286852012 hasOpenAccess W4286852012 @default.
- W4286852012 hasPrimaryLocation W42868520121 @default.
- W4286852012 hasRelatedWork W1543053641 @default.
- W4286852012 hasRelatedWork W1675599972 @default.
- W4286852012 hasRelatedWork W1979570124 @default.
- W4286852012 hasRelatedWork W2010254549 @default.
- W4286852012 hasRelatedWork W2028518625 @default.
- W4286852012 hasRelatedWork W2042678520 @default.
- W4286852012 hasRelatedWork W2075729495 @default.
- W4286852012 hasRelatedWork W2079053718 @default.
- W4286852012 hasRelatedWork W2902899725 @default.
- W4286852012 hasRelatedWork W3169834474 @default.
- W4286852012 isParatext "false" @default.
- W4286852012 isRetracted "false" @default.
- W4286852012 workType "article" @default.