FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4286945596> ?p ?o ?g. }

• W4286945596 endingPage "1747" @default.
• W4286945596 startingPage "1741" @default.
• W4286945596 abstract "Abstract Background Keratinocyte culture is a standard method used to study gene expression, cell differentiation and proliferation. Numerous protocols exist, however their application is frequently unsuitable for small specimens, such as 4‐mm punch skin biopsies. Aims This study compared 3 different methods of keratinocyte culture from paediatric skin biopsies to evaluate which one ensures adequate cell growth for RNA extraction and sequencing. Materials and methods Thirty‐six skin samples were obtained from 4‐mm punch skin biopsies from residual human body material from healthy children. They were cultured in vitro according to 3 different methods: enzymatic method, epidermis explant and direct explant method. Keratinocytes were characterized by immunocytochemistry using pan‐cytokeratin. RNA extraction was performed with RNeasy Mini kit. Quantity and quality of the extracted RNA was assessed to meet the requirements of library preparation for sequencing. Results The direct explant method had largely shown its superiority over the two other methods, with a 100% success rate and an average of 15 days of culture. RNA extraction yielded a mean of 8545.85 ng of RNA per sample with an RQN of 10. Cover‐clip immunochemistry staining with pan‐cytokeratin had confirmed the absence of fibroblast contamination. Discussion Although the enzymatic method is the most frequently used for keratinocyte culture, it is not suitable small samples required in dermatology. The direct explant method guarantees a high growth rate and the extraction of high quality RNA. Variation in the amount of RNA harvested are related to inter‐ and intra‐individual variations and to the conditions of the experiment. Conclusion This study allowed to conclude that the direct explant method is the most efficient and easy method to ensure cell growth when the samples are from 4‐mm punch skin biopsies. This technique avoids fibroblasts contamination and obtains a sufficient quantity and quality of RNA to sequence it." @default.
• W4286945596 created "2022-07-25" @default.
• W4286945596 creator A5015321260 @default.
• W4286945596 creator A5018984115 @default.
• W4286945596 creator A5022305264 @default.
• W4286945596 creator A5049160636 @default.
• W4286945596 creator A5084864179 @default.
• W4286945596 creator A5085405059 @default.
• W4286945596 date "2022-07-31" @default.
• W4286945596 modified "2023-09-26" @default.
• W4286945596 title "Comparative study of keratinocyte primary culture methods from paediatric skin biopsies for RNA‐sequencing" @default.
• W4286945596 cites W1552066155 @default.
• W4286945596 cites W1982023029 @default.
• W4286945596 cites W1988702122 @default.
• W4286945596 cites W2013108964 @default.
• W4286945596 cites W2032460464 @default.
• W4286945596 cites W2049043712 @default.
• W4286945596 cites W2053498796 @default.
• W4286945596 cites W2063845786 @default.
• W4286945596 cites W2070942486 @default.
• W4286945596 cites W2074871118 @default.
• W4286945596 cites W2075198678 @default.
• W4286945596 cites W2091291827 @default.
• W4286945596 cites W2137231640 @default.
• W4286945596 cites W2617843944 @default.
• W4286945596 cites W2801546295 @default.
• W4286945596 cites W2929533727 @default.
• W4286945596 cites W2968815091 @default.
• W4286945596 cites W2970262083 @default.
• W4286945596 cites W2985957135 @default.
• W4286945596 cites W2996053991 @default.
• W4286945596 cites W2997387145 @default.
• W4286945596 cites W2998908226 @default.
• W4286945596 cites W3026332377 @default.
• W4286945596 cites W3208256252 @default.
• W4286945596 doi "https://doi.org/10.1111/exd.14652" @default.
• W4286945596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35871540" @default.
• W4286945596 hasPublicationYear "2022" @default.
• W4286945596 type Work @default.
• W4286945596 citedByCount "2" @default.
• W4286945596 countsByYear W42869455962023 @default.
• W4286945596 crossrefType "journal-article" @default.
• W4286945596 hasAuthorship W4286945596A5015321260 @default.
• W4286945596 hasAuthorship W4286945596A5018984115 @default.
• W4286945596 hasAuthorship W4286945596A5022305264 @default.
• W4286945596 hasAuthorship W4286945596A5049160636 @default.
• W4286945596 hasAuthorship W4286945596A5084864179 @default.
• W4286945596 hasAuthorship W4286945596A5085405059 @default.
• W4286945596 hasConcept C104317684 @default.
• W4286945596 hasConcept C105702510 @default.
• W4286945596 hasConcept C112190416 @default.
• W4286945596 hasConcept C142724271 @default.
• W4286945596 hasConcept C153911025 @default.
• W4286945596 hasConcept C157890192 @default.
• W4286945596 hasConcept C202751555 @default.
• W4286945596 hasConcept C203014093 @default.
• W4286945596 hasConcept C204232928 @default.
• W4286945596 hasConcept C205713591 @default.
• W4286945596 hasConcept C2776458125 @default.
• W4286945596 hasConcept C2777074287 @default.
• W4286945596 hasConcept C2779012181 @default.
• W4286945596 hasConcept C55493867 @default.
• W4286945596 hasConcept C67705224 @default.
• W4286945596 hasConcept C71924100 @default.
• W4286945596 hasConcept C86803240 @default.
• W4286945596 hasConceptScore W4286945596C104317684 @default.
• W4286945596 hasConceptScore W4286945596C105702510 @default.
• W4286945596 hasConceptScore W4286945596C112190416 @default.
• W4286945596 hasConceptScore W4286945596C142724271 @default.
• W4286945596 hasConceptScore W4286945596C153911025 @default.
• W4286945596 hasConceptScore W4286945596C157890192 @default.
• W4286945596 hasConceptScore W4286945596C202751555 @default.
• W4286945596 hasConceptScore W4286945596C203014093 @default.
• W4286945596 hasConceptScore W4286945596C204232928 @default.
• W4286945596 hasConceptScore W4286945596C205713591 @default.
• W4286945596 hasConceptScore W4286945596C2776458125 @default.
• W4286945596 hasConceptScore W4286945596C2777074287 @default.
• W4286945596 hasConceptScore W4286945596C2779012181 @default.
• W4286945596 hasConceptScore W4286945596C55493867 @default.
• W4286945596 hasConceptScore W4286945596C67705224 @default.
• W4286945596 hasConceptScore W4286945596C71924100 @default.
• W4286945596 hasConceptScore W4286945596C86803240 @default.
• W4286945596 hasIssue "11" @default.
• W4286945596 hasLocation W42869455961 @default.
• W4286945596 hasLocation W42869455962 @default.
• W4286945596 hasOpenAccess W4286945596 @default.
• W4286945596 hasPrimaryLocation W42869455961 @default.
• W4286945596 hasRelatedWork W1982203362 @default.
• W4286945596 hasRelatedWork W2348628032 @default.
• W4286945596 hasRelatedWork W2363707985 @default.
• W4286945596 hasRelatedWork W2364543258 @default.
• W4286945596 hasRelatedWork W2365126566 @default.
• W4286945596 hasRelatedWork W2367351498 @default.
• W4286945596 hasRelatedWork W2806739216 @default.
• W4286945596 hasRelatedWork W3102962722 @default.
• W4286945596 hasRelatedWork W4280539041 @default.
• W4286945596 hasRelatedWork W4286945596 @default.
• W4286945596 hasVolume "31" @default.

• next