Matches in SemOpenAlex for { <https://semopenalex.org/work/W4287121352> ?p ?o ?g. }
Showing items 1 to 53 of
53
with 100 items per page.
- W4287121352 abstract "Fibro-adipogenic Progenitors (FAPs) are resident interstitial cells in skeletal muscle that, together with myogenic progenitors (MPs), play a key role in muscle homeostasis, injury, and repair. Current protocols for FAPs identification and isolation use flow cytometry/fluorescence-activated cell sorting (FACS) and studies evaluating their function in vivo to date have been undertaken exclusively in mice. The larger inherent size of the rat allows for a more comprehensive analysis of FAPs in skeletal muscle injury models, especially in severely atrophic muscle or when investigators require substantial tissue mass to conduct multiple downstream assays. The rat additionally provides a larger selection of muscle functional assays that do not require animal sedation or sacrifice, thus minimizing morbidity and animal use by enabling serial assessments. The flow cytometry/FACS protocols optimized for mice are species specific, notably restricted by the characteristics of commercially available antibodies. They have not been optimized for separating FAPs from rat or highly fibrotic muscle. A flow cytometry/FACS protocol for the identification and isolation of FAPs and MPs from both healthy and denervated rat skeletal muscle was developed, relying on the differential expression of surface markers CD31, CD45, Sca-1, and VCAM-1. As rat-specific, flow cytometry-validated primary antibodies are severely limited, in-house conjugation of the antibody targeting Sca-1 was performed. Using this protocol, successful Sca-1 conjugation was confirmed, and flow cytometric identification of FAPs and MPs was validated by cell culture and immunostaining of FACS-isolated FAPs and MPs. Finally, we report a novel FAPs time-course in a prolonged (14 week) rat denervation model. This method provides the investigators the ability to study FAPs in a novel animal model." @default.
- W4287121352 created "2022-07-25" @default.
- W4287121352 creator A5007616821 @default.
- W4287121352 creator A5066582610 @default.
- W4287121352 creator A5086187566 @default.
- W4287121352 creator A5087820805 @default.
- W4287121352 date "2021-06-09" @default.
- W4287121352 modified "2023-09-23" @default.
- W4287121352 title "Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat" @default.
- W4287121352 doi "https://doi.org/10.3791/61750-v" @default.
- W4287121352 hasPublicationYear "2021" @default.
- W4287121352 type Work @default.
- W4287121352 citedByCount "0" @default.
- W4287121352 crossrefType "journal-article" @default.
- W4287121352 hasAuthorship W4287121352A5007616821 @default.
- W4287121352 hasAuthorship W4287121352A5066582610 @default.
- W4287121352 hasAuthorship W4287121352A5086187566 @default.
- W4287121352 hasAuthorship W4287121352A5087820805 @default.
- W4287121352 hasConcept C105702510 @default.
- W4287121352 hasConcept C201750760 @default.
- W4287121352 hasConcept C203014093 @default.
- W4287121352 hasConcept C2778630268 @default.
- W4287121352 hasConcept C2779959927 @default.
- W4287121352 hasConcept C28328180 @default.
- W4287121352 hasConcept C553184892 @default.
- W4287121352 hasConcept C86803240 @default.
- W4287121352 hasConcept C95444343 @default.
- W4287121352 hasConceptScore W4287121352C105702510 @default.
- W4287121352 hasConceptScore W4287121352C201750760 @default.
- W4287121352 hasConceptScore W4287121352C203014093 @default.
- W4287121352 hasConceptScore W4287121352C2778630268 @default.
- W4287121352 hasConceptScore W4287121352C2779959927 @default.
- W4287121352 hasConceptScore W4287121352C28328180 @default.
- W4287121352 hasConceptScore W4287121352C553184892 @default.
- W4287121352 hasConceptScore W4287121352C86803240 @default.
- W4287121352 hasConceptScore W4287121352C95444343 @default.
- W4287121352 hasIssue "172" @default.
- W4287121352 hasLocation W42871213521 @default.
- W4287121352 hasOpenAccess W4287121352 @default.
- W4287121352 hasPrimaryLocation W42871213521 @default.
- W4287121352 hasRelatedWork W2048957342 @default.
- W4287121352 hasRelatedWork W2135793203 @default.
- W4287121352 hasRelatedWork W2300366807 @default.
- W4287121352 hasRelatedWork W2319078064 @default.
- W4287121352 hasRelatedWork W2405513515 @default.
- W4287121352 hasRelatedWork W2589262745 @default.
- W4287121352 hasRelatedWork W2801524442 @default.
- W4287121352 hasRelatedWork W2942732239 @default.
- W4287121352 hasRelatedWork W3213450778 @default.
- W4287121352 hasRelatedWork W4200619956 @default.
- W4287121352 isParatext "false" @default.
- W4287121352 isRetracted "false" @default.
- W4287121352 workType "article" @default.