Matches in SemOpenAlex for { <https://semopenalex.org/work/W4287180879> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W4287180879 abstract "Thermal shift assays (TSAs) examine how the melting temperature (Tm) of a target protein changes in response to changes in its environment (e.g., buffer composition). The utility of TSA, and specifically of nano-Differential Scanning Fluorimetry (nano-DSF), has been established over the years, both for finding conditions that help stabilize a specific protein and for looking at ligand binding by monitoring changes in the apparent Tm. This paper presents an efficient screening of the Diamond-SGC-iNEXT Poised (DSi-Poised) fragment library (768 compounds) by the use of nano-DSF, monitoring Tm to identify potential fragment binding. The prerequisites regarding protein quality and concentration for performing nano-DSF experiments are briefly outlined followed by a step-by-step protocol that uses a nano-liter robotic dispenser commonly used in structural biology laboratories for preparing the required samples in 96-well plates. The protocol describes how the reagent mixtures are transferred to the capillaries needed for nano-DSF measurements. In addition, this paper provides protocols to measure thermal denaturation (monitoring intrinsic tryptophan fluorescence) and aggregation (monitoring light back-scattering) and the subsequent steps for data transfer and analysis. Finally, screening experiments with three different protein targets are discussed to illustrate the use of this procedure in the context of lead discovery campaigns. The overall principle of the method described can be easily transferred to other fragment libraries or adapted to other instruments." @default.
- W4287180879 created "2022-07-25" @default.
- W4287180879 creator A5014431830 @default.
- W4287180879 creator A5015693995 @default.
- W4287180879 creator A5024945543 @default.
- W4287180879 creator A5031178846 @default.
- W4287180879 creator A5032564649 @default.
- W4287180879 creator A5038389197 @default.
- W4287180879 creator A5063171176 @default.
- W4287180879 creator A5065902823 @default.
- W4287180879 creator A5073065359 @default.
- W4287180879 date "2021-05-16" @default.
- W4287180879 modified "2023-10-01" @default.
- W4287180879 title "Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery" @default.
- W4287180879 doi "https://doi.org/10.3791/62469-v" @default.
- W4287180879 hasPublicationYear "2021" @default.
- W4287180879 type Work @default.
- W4287180879 citedByCount "0" @default.
- W4287180879 crossrefType "journal-article" @default.
- W4287180879 hasAuthorship W4287180879A5014431830 @default.
- W4287180879 hasAuthorship W4287180879A5015693995 @default.
- W4287180879 hasAuthorship W4287180879A5024945543 @default.
- W4287180879 hasAuthorship W4287180879A5031178846 @default.
- W4287180879 hasAuthorship W4287180879A5032564649 @default.
- W4287180879 hasAuthorship W4287180879A5038389197 @default.
- W4287180879 hasAuthorship W4287180879A5063171176 @default.
- W4287180879 hasAuthorship W4287180879A5065902823 @default.
- W4287180879 hasAuthorship W4287180879A5073065359 @default.
- W4287180879 hasBestOaLocation W42871808791 @default.
- W4287180879 hasConcept C11413529 @default.
- W4287180879 hasConcept C120665830 @default.
- W4287180879 hasConcept C121332964 @default.
- W4287180879 hasConcept C12554922 @default.
- W4287180879 hasConcept C142724271 @default.
- W4287180879 hasConcept C151730666 @default.
- W4287180879 hasConcept C158711907 @default.
- W4287180879 hasConcept C159985019 @default.
- W4287180879 hasConcept C171250308 @default.
- W4287180879 hasConcept C185592680 @default.
- W4287180879 hasConcept C186060115 @default.
- W4287180879 hasConcept C192562407 @default.
- W4287180879 hasConcept C204787440 @default.
- W4287180879 hasConcept C2776235265 @default.
- W4287180879 hasConcept C2779343474 @default.
- W4287180879 hasConcept C2780357685 @default.
- W4287180879 hasConcept C2780385302 @default.
- W4287180879 hasConcept C41008148 @default.
- W4287180879 hasConcept C70721500 @default.
- W4287180879 hasConcept C71924100 @default.
- W4287180879 hasConcept C86803240 @default.
- W4287180879 hasConcept C91881484 @default.
- W4287180879 hasConceptScore W4287180879C11413529 @default.
- W4287180879 hasConceptScore W4287180879C120665830 @default.
- W4287180879 hasConceptScore W4287180879C121332964 @default.
- W4287180879 hasConceptScore W4287180879C12554922 @default.
- W4287180879 hasConceptScore W4287180879C142724271 @default.
- W4287180879 hasConceptScore W4287180879C151730666 @default.
- W4287180879 hasConceptScore W4287180879C158711907 @default.
- W4287180879 hasConceptScore W4287180879C159985019 @default.
- W4287180879 hasConceptScore W4287180879C171250308 @default.
- W4287180879 hasConceptScore W4287180879C185592680 @default.
- W4287180879 hasConceptScore W4287180879C186060115 @default.
- W4287180879 hasConceptScore W4287180879C192562407 @default.
- W4287180879 hasConceptScore W4287180879C204787440 @default.
- W4287180879 hasConceptScore W4287180879C2776235265 @default.
- W4287180879 hasConceptScore W4287180879C2779343474 @default.
- W4287180879 hasConceptScore W4287180879C2780357685 @default.
- W4287180879 hasConceptScore W4287180879C2780385302 @default.
- W4287180879 hasConceptScore W4287180879C41008148 @default.
- W4287180879 hasConceptScore W4287180879C70721500 @default.
- W4287180879 hasConceptScore W4287180879C71924100 @default.
- W4287180879 hasConceptScore W4287180879C86803240 @default.
- W4287180879 hasConceptScore W4287180879C91881484 @default.
- W4287180879 hasIssue "171" @default.
- W4287180879 hasLocation W42871808791 @default.
- W4287180879 hasOpenAccess W4287180879 @default.
- W4287180879 hasPrimaryLocation W42871808791 @default.
- W4287180879 hasRelatedWork W1998831308 @default.
- W4287180879 hasRelatedWork W2029538319 @default.
- W4287180879 hasRelatedWork W2034173185 @default.
- W4287180879 hasRelatedWork W2050538415 @default.
- W4287180879 hasRelatedWork W2070061241 @default.
- W4287180879 hasRelatedWork W2080319452 @default.
- W4287180879 hasRelatedWork W2085590670 @default.
- W4287180879 hasRelatedWork W2619318515 @default.
- W4287180879 hasRelatedWork W2952230677 @default.
- W4287180879 hasRelatedWork W4200216331 @default.
- W4287180879 isParatext "false" @default.
- W4287180879 isRetracted "false" @default.
- W4287180879 workType "article" @default.