Matches in SemOpenAlex for { <https://semopenalex.org/work/W4287201230> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W4287201230 abstract "Disruption of the glomerular filter composed of the glomerular endothelium, glomerular basement membrane and podocytes, results in albuminuria. Podocyte foot processes contain actin bundles that bind to cytoskeletal adaptor proteins such as podocin. Those adaptor proteins, such as podocin, link the backbone of the glomerular slit diaphragm, such as nephrin, to the actin cytoskeleton. Studying the localization and function of these and other podocytic proteins is essential for the understanding of the glomerular filter's role in health and disease. The presented protocol enables the user to visualize actin, podocin, and nephrin in cells with super resolution imaging on a conventional microscope. First, cells are stained with a conventional immunofluorescence technique. All proteins within the sample are then covalently anchored to a swellable hydrogel. Through digestion with proteinase K, structural proteins are cleaved allowing isotropical swelling of the gel in the last step. Dialysis of the sample in water results in a 4-4.5-fold expansion of the sample and the sample can be imaged via a conventional fluorescence microscope, rendering a potential resolution of 70 nm." @default.
- W4287201230 created "2022-07-25" @default.
- W4287201230 creator A5042460929 @default.
- W4287201230 creator A5068721240 @default.
- W4287201230 creator A5068829798 @default.
- W4287201230 creator A5082149512 @default.
- W4287201230 date "2021-04-23" @default.
- W4287201230 modified "2023-09-30" @default.
- W4287201230 title "Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy" @default.
- W4287201230 doi "https://doi.org/10.3791/62079-v" @default.
- W4287201230 hasPublicationYear "2021" @default.
- W4287201230 type Work @default.
- W4287201230 citedByCount "0" @default.
- W4287201230 crossrefType "journal-article" @default.
- W4287201230 hasAuthorship W4287201230A5042460929 @default.
- W4287201230 hasAuthorship W4287201230A5068721240 @default.
- W4287201230 hasAuthorship W4287201230A5068829798 @default.
- W4287201230 hasAuthorship W4287201230A5082149512 @default.
- W4287201230 hasConcept C12554922 @default.
- W4287201230 hasConcept C134018914 @default.
- W4287201230 hasConcept C142669718 @default.
- W4287201230 hasConcept C1491633281 @default.
- W4287201230 hasConcept C185592680 @default.
- W4287201230 hasConcept C2776115139 @default.
- W4287201230 hasConcept C2776855237 @default.
- W4287201230 hasConcept C2777146197 @default.
- W4287201230 hasConcept C2777979848 @default.
- W4287201230 hasConcept C2779561371 @default.
- W4287201230 hasConcept C2780091579 @default.
- W4287201230 hasConcept C2780368995 @default.
- W4287201230 hasConcept C2780819817 @default.
- W4287201230 hasConcept C2781156571 @default.
- W4287201230 hasConcept C2993400109 @default.
- W4287201230 hasConcept C3018201436 @default.
- W4287201230 hasConcept C55493867 @default.
- W4287201230 hasConcept C86803240 @default.
- W4287201230 hasConcept C95444343 @default.
- W4287201230 hasConceptScore W4287201230C12554922 @default.
- W4287201230 hasConceptScore W4287201230C134018914 @default.
- W4287201230 hasConceptScore W4287201230C142669718 @default.
- W4287201230 hasConceptScore W4287201230C1491633281 @default.
- W4287201230 hasConceptScore W4287201230C185592680 @default.
- W4287201230 hasConceptScore W4287201230C2776115139 @default.
- W4287201230 hasConceptScore W4287201230C2776855237 @default.
- W4287201230 hasConceptScore W4287201230C2777146197 @default.
- W4287201230 hasConceptScore W4287201230C2777979848 @default.
- W4287201230 hasConceptScore W4287201230C2779561371 @default.
- W4287201230 hasConceptScore W4287201230C2780091579 @default.
- W4287201230 hasConceptScore W4287201230C2780368995 @default.
- W4287201230 hasConceptScore W4287201230C2780819817 @default.
- W4287201230 hasConceptScore W4287201230C2781156571 @default.
- W4287201230 hasConceptScore W4287201230C2993400109 @default.
- W4287201230 hasConceptScore W4287201230C3018201436 @default.
- W4287201230 hasConceptScore W4287201230C55493867 @default.
- W4287201230 hasConceptScore W4287201230C86803240 @default.
- W4287201230 hasConceptScore W4287201230C95444343 @default.
- W4287201230 hasIssue "170" @default.
- W4287201230 hasLocation W42872012301 @default.
- W4287201230 hasOpenAccess W4287201230 @default.
- W4287201230 hasPrimaryLocation W42872012301 @default.
- W4287201230 hasRelatedWork W1540786657 @default.
- W4287201230 hasRelatedWork W2009109782 @default.
- W4287201230 hasRelatedWork W2041215839 @default.
- W4287201230 hasRelatedWork W2046549589 @default.
- W4287201230 hasRelatedWork W2056995253 @default.
- W4287201230 hasRelatedWork W2059264239 @default.
- W4287201230 hasRelatedWork W2141525487 @default.
- W4287201230 hasRelatedWork W3043287600 @default.
- W4287201230 hasRelatedWork W4210445361 @default.
- W4287201230 hasRelatedWork W2530339862 @default.
- W4287201230 isParatext "false" @default.
- W4287201230 isRetracted "false" @default.
- W4287201230 workType "article" @default.