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- W4287306363 abstract "Detection of pyrophosphate is important in quantifying enzyme activity, particularly adenylation domain activity during non-ribosomal peptide synthesis. The previous development of an enzyme coupled PPi /NADH assay allowed the measurement of such activity in an online fashion using commercially available components. Now, with a key enzyme - 6-phosphofructokinase - no longer available, we have screened and identified viable replacement enzymes that can be expressed in high yield and that are far superior in activity to the now discontinued commercial product. This will support the ability of groups to continue to use this established online assay for pyrophosphate detection." @default.
- W4287306363 created "2022-07-25" @default.
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- W4287306363 date "2022-07-25" @default.
- W4287306363 modified "2023-10-14" @default.
- W4287306363 title "Replacing Commercial 6‐Phosphofructokinase in an Online Pyrophosphate Detection Assay" @default.
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- W4287306363 doi "https://doi.org/10.1002/cbic.202200325" @default.
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