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• W4287447079 abstract "Herein, we detail a novel reverse-transcription (RT) assay to directly detect chemical adducts on RNA. We optimize a fluorescence quenching assay to detect RT polymerization and employ our approach to detect N1-alkylation of inosine, an important post-transcriptional modification, using a phenylacrylamide as a model compound. We anticipate our approach can be expanded to identify novel reagents that form adducts with RNA and further explored to understand the relationship between RT processivity and natural post-transcriptional modifications in RNA." @default.
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• W4287447079 date "2022-07-25" @default.
• W4287447079 modified "2023-09-30" @default.
• W4287447079 title "A Fluorescent Reverse-Transcription Assay to Detect Chemical Adducts on RNA" @default.
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• W4287447079 doi "https://doi.org/10.1021/acs.biochem.2c00270" @default.
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