Matches in SemOpenAlex for { <https://semopenalex.org/work/W4287510521> ?p ?o ?g. }
- W4287510521 endingPage "1064" @default.
- W4287510521 startingPage "1056" @default.
- W4287510521 abstract "SARS-CoV-2 entry into cells requires specific host proteases; however, no successful in vivo applications of host protease inhibitors have yet been reported for treatment of SARS-CoV-2 pathogenesis. Here we describe a chemically engineered nanosystem encapsulating CRISPR-Cas13d, developed to specifically target lung protease cathepsin L (Ctsl) messenger RNA to block SARS-CoV-2 infection in mice. We show that this nanosystem decreases lung Ctsl expression in normal mice efficiently, specifically and safely. We further show that this approach extends survival of mice lethally infected with SARS-CoV-2, correlating with decreased lung virus burden, reduced expression of proinflammatory cytokines/chemokines and diminished severity of pulmonary interstitial inflammation. Postinfection treatment by this nanosystem dramatically lowers the lung virus burden and alleviates virus-induced pathological changes. Our results indicate that targeting lung protease mRNA by Cas13d nanosystem represents a unique strategy for controlling SARS-CoV-2 infection and demonstrate that CRISPR can be used as a potential treatment for SARS-CoV-2 infection." @default.
- W4287510521 created "2022-07-25" @default.
- W4287510521 creator A5009455182 @default.
- W4287510521 creator A5012390831 @default.
- W4287510521 creator A5022713049 @default.
- W4287510521 creator A5023285963 @default.
- W4287510521 creator A5026394330 @default.
- W4287510521 creator A5026793684 @default.
- W4287510521 creator A5029912396 @default.
- W4287510521 creator A5035281555 @default.
- W4287510521 creator A5039431143 @default.
- W4287510521 creator A5047253579 @default.
- W4287510521 creator A5047863309 @default.
- W4287510521 creator A5049021093 @default.
- W4287510521 creator A5062868933 @default.
- W4287510521 creator A5066355670 @default.
- W4287510521 creator A5087681431 @default.
- W4287510521 creator A5091135797 @default.
- W4287510521 creator A5091488989 @default.
- W4287510521 date "2022-07-25" @default.
- W4287510521 modified "2023-10-17" @default.
- W4287510521 title "Cas13d knockdown of lung protease Ctsl prevents and treats SARS-CoV-2 infection" @default.
- W4287510521 cites W1607422675 @default.
- W4287510521 cites W2006286994 @default.
- W4287510521 cites W2010912389 @default.
- W4287510521 cites W2017689556 @default.
- W4287510521 cites W2043828851 @default.
- W4287510521 cites W2055480930 @default.
- W4287510521 cites W2081719521 @default.
- W4287510521 cites W2083106544 @default.
- W4287510521 cites W2107012923 @default.
- W4287510521 cites W2260024370 @default.
- W4287510521 cites W2285232484 @default.
- W4287510521 cites W2412158473 @default.
- W4287510521 cites W2765351619 @default.
- W4287510521 cites W2793359586 @default.
- W4287510521 cites W2795445828 @default.
- W4287510521 cites W2806280499 @default.
- W4287510521 cites W2889370330 @default.
- W4287510521 cites W2914425913 @default.
- W4287510521 cites W2978981832 @default.
- W4287510521 cites W3009912996 @default.
- W4287510521 cites W3013093478 @default.
- W4287510521 cites W3015140168 @default.
- W4287510521 cites W3016048690 @default.
- W4287510521 cites W3021577619 @default.
- W4287510521 cites W3022243369 @default.
- W4287510521 cites W3022285953 @default.
- W4287510521 cites W3027630905 @default.
- W4287510521 cites W3032297724 @default.
- W4287510521 cites W3041901315 @default.
- W4287510521 cites W3080378920 @default.
- W4287510521 cites W3080981445 @default.
- W4287510521 cites W3092477062 @default.
- W4287510521 cites W3093900830 @default.
- W4287510521 cites W3094597057 @default.
- W4287510521 cites W3099347064 @default.
- W4287510521 cites W3107191813 @default.
- W4287510521 cites W3120168938 @default.
- W4287510521 cites W3123064250 @default.
- W4287510521 cites W3126161304 @default.
- W4287510521 cites W3128627243 @default.
- W4287510521 cites W3139099049 @default.
- W4287510521 cites W3151076337 @default.
- W4287510521 cites W3155123963 @default.
- W4287510521 cites W3157786842 @default.
- W4287510521 cites W3161032756 @default.
- W4287510521 cites W3161773261 @default.
- W4287510521 cites W3175961346 @default.
- W4287510521 cites W3198233589 @default.
- W4287510521 cites W3205652863 @default.
- W4287510521 cites W3214054621 @default.
- W4287510521 cites W4200051875 @default.
- W4287510521 cites W4210665241 @default.
- W4287510521 cites W4221056874 @default.
- W4287510521 cites W4221072241 @default.
- W4287510521 cites W4226236384 @default.
- W4287510521 doi "https://doi.org/10.1038/s41589-022-01094-4" @default.
- W4287510521 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35879545" @default.
- W4287510521 hasPublicationYear "2022" @default.
- W4287510521 type Work @default.
- W4287510521 citedByCount "15" @default.
- W4287510521 countsByYear W42875105212022 @default.
- W4287510521 countsByYear W42875105212023 @default.
- W4287510521 crossrefType "journal-article" @default.
- W4287510521 hasAuthorship W4287510521A5009455182 @default.
- W4287510521 hasAuthorship W4287510521A5012390831 @default.
- W4287510521 hasAuthorship W4287510521A5022713049 @default.
- W4287510521 hasAuthorship W4287510521A5023285963 @default.
- W4287510521 hasAuthorship W4287510521A5026394330 @default.
- W4287510521 hasAuthorship W4287510521A5026793684 @default.
- W4287510521 hasAuthorship W4287510521A5029912396 @default.
- W4287510521 hasAuthorship W4287510521A5035281555 @default.
- W4287510521 hasAuthorship W4287510521A5039431143 @default.
- W4287510521 hasAuthorship W4287510521A5047253579 @default.
- W4287510521 hasAuthorship W4287510521A5047863309 @default.
- W4287510521 hasAuthorship W4287510521A5049021093 @default.
- W4287510521 hasAuthorship W4287510521A5062868933 @default.