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- W4287901211 abstract "We investigate rotational diffusion of fluorescent molecules in angular potential wells, the excitation and subsequent emissions from these diffusing molecules, and the imaging of these emissions with high-NA aplanatic optical microscopes. Although dipole emissions only transmit six low-frequency angular components, we show that angular structured illumination can alias higher frequency angular components into the passband of the imaging system. We show that the number of measurable angular components is limited by the relationships between three time scales: the rotational diffusion time, the fluorescence decay time, and the acquisition time. We demonstrate our model by simulating a numerical phantom in the limits of fast angular diffusion, slow angular diffusion, and weak potentials." @default.
- W4287901211 created "2022-07-26" @default.
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- W4287901211 date "2020-01-24" @default.
- W4287901211 modified "2023-10-03" @default.
- W4287901211 title "Spatio-angular fluorescence microscopy III. Constrained angular diffusion, polarized excitation, and high-NA imaging" @default.
- W4287901211 doi "https://doi.org/10.48550/arxiv.2001.09232" @default.
- W4287901211 hasPublicationYear "2020" @default.
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