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- W4287930183 abstract "As fossil fuels are declining, global economies need sustainable energy sources. Biofuel, the most promising candidate for the energy crisis, is still not competitive as compared to fossil fuels. Thus, higher yields of biofuel and simultaneous production of high-value commodities (HVCs) to compensate for the production cost are a plausible strategy. Microalgae in this context are a successful candidate, due to their ability to produce various nutraceuticals, pharmaceuticals, and many other HVCs alongside biofuels. Higher sequestration of greenhouse gases (GHGs) to biomass and the ability to recycle nutrient-rich cosmopolitan waste with a biorefinery-based approach give microalgae an advantage over other photoautotrophs. This biofuel and HVC-rich biomass can be further enhanced by regulating some specific targets like pathway genes, transcription factors, enhancers, and repressors. Zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and Clustered regulatory interspaced short palindromic repeats /CRISPR-associated protein 9 (CRISPR/Cas9) are some technologies used for this purpose. The combination of these synthetic biology approaches with bioprocess improvement can help in increasing biofuel and HVC production. However, the unavailability of microalgal genomes is a bottleneck in their genetic engineering for a sustainable biorefinery. The advances in multi-omics technologies give possible solutions for effectively understanding the microalgal genomes. Thus, in this chapter, we discuss microalgal physiology in light of synthetic biology and aforementioned genome editing techniques in combination with major bottlenecks and their solutions." @default.
- W4287930183 created "2022-07-26" @default.
- W4287930183 creator A5006268071 @default.
- W4287930183 creator A5052836692 @default.
- W4287930183 creator A5057290314 @default.
- W4287930183 date "2022-01-01" @default.
- W4287930183 modified "2023-10-14" @default.
- W4287930183 title "Synthetic Biology-Based Advanced Biotechnological Approach in Microalgal Biorefinery" @default.
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