Matches in SemOpenAlex for { <https://semopenalex.org/work/W4287987652> ?p ?o ?g. }
- W4287987652 endingPage "109" @default.
- W4287987652 startingPage "81" @default.
- W4287987652 abstract "Philadelphia-negative myeloproliferative neoplasms (pH-MPNs) origin from the clonal expansion of hematopoietic stem cells with acquired mutations leading to uncontrolled proliferation of differentiated myeloid cells. The main entities of Ph-MPNs are represented by Polycythemia Vera (PV), Essential Thrombocythemia (ET) and Myelofibrosis (MF) that are characterized by microvascular disorders, thrombosis and bleeding, splenomegaly secondary to extramedullary hematopoiesis, various degree of bone marrow fibrosis and a progressive risk of leukemic transformation. Somatic mutations in myeloid genes including JAK2, CALR, and MPL cause the constitutive activation of the Janus Kinase 2 (JAK)/signal transducer and activator of transcription (STAT) pathway that confers proliferative and differentiative advantage to mutated hematopoietic progenitors and ultimately drives the development of a Ph-MPNs phenotype. Beyond the JAK/STAT axis, a wide number of intracellular signaling pathways were found deregulated in Ph-MPNs including the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) constitutive activation. In this chapter, we provide a detailed protocol for the immunoblotting assisted assessment of Ph-MPNs pathways activation. This protocol can be easily adapted to study protein expression and phosphorylation of hematopoietic stem progenitors and differentiated cell lineages." @default.
- W4287987652 created "2022-07-26" @default.
- W4287987652 creator A5038401147 @default.
- W4287987652 creator A5056606171 @default.
- W4287987652 creator A5059744998 @default.
- W4287987652 date "2022-01-01" @default.
- W4287987652 modified "2023-10-18" @default.
- W4287987652 title "Immunoblotting-assisted assessment of JAK/STAT and PI3K/Akt/mTOR signaling in myeloproliferative neoplasms CD34+ stem cells" @default.
- W4287987652 cites W1978853026 @default.
- W4287987652 cites W1980568348 @default.
- W4287987652 cites W1980642919 @default.
- W4287987652 cites W1985809519 @default.
- W4287987652 cites W1992604276 @default.
- W4287987652 cites W1995981641 @default.
- W4287987652 cites W1998593414 @default.
- W4287987652 cites W2000900457 @default.
- W4287987652 cites W2028390656 @default.
- W4287987652 cites W2029318743 @default.
- W4287987652 cites W2030569551 @default.
- W4287987652 cites W2030868349 @default.
- W4287987652 cites W2036637470 @default.
- W4287987652 cites W2045945994 @default.
- W4287987652 cites W2046567666 @default.
- W4287987652 cites W2049140059 @default.
- W4287987652 cites W2049145190 @default.
- W4287987652 cites W2054792541 @default.
- W4287987652 cites W2055096888 @default.
- W4287987652 cites W2056777053 @default.
- W4287987652 cites W2057360098 @default.
- W4287987652 cites W2075545369 @default.
- W4287987652 cites W2078743099 @default.
- W4287987652 cites W2082449404 @default.
- W4287987652 cites W2084050646 @default.
- W4287987652 cites W2091155023 @default.
- W4287987652 cites W2093301943 @default.
- W4287987652 cites W2094290642 @default.
- W4287987652 cites W2094457899 @default.
- W4287987652 cites W2116878961 @default.
- W4287987652 cites W2117986250 @default.
- W4287987652 cites W2118995216 @default.
- W4287987652 cites W2138709799 @default.
- W4287987652 cites W2144664431 @default.
- W4287987652 cites W2146505525 @default.
- W4287987652 cites W2157694607 @default.
- W4287987652 cites W2166709785 @default.
- W4287987652 cites W2176681260 @default.
- W4287987652 cites W2230798701 @default.
- W4287987652 cites W2339328455 @default.
- W4287987652 cites W2339658711 @default.
- W4287987652 cites W2344384041 @default.
- W4287987652 cites W2346845651 @default.
- W4287987652 cites W2417685891 @default.
- W4287987652 cites W2548605737 @default.
- W4287987652 cites W2620018935 @default.
- W4287987652 cites W2790003237 @default.
- W4287987652 cites W2790425028 @default.
- W4287987652 cites W2963608123 @default.
- W4287987652 cites W2981465698 @default.
- W4287987652 cites W3000101033 @default.
- W4287987652 cites W3026423379 @default.
- W4287987652 cites W3043783835 @default.
- W4287987652 cites W3045772015 @default.
- W4287987652 cites W3155772349 @default.
- W4287987652 cites W3174383320 @default.
- W4287987652 cites W3196753640 @default.
- W4287987652 cites W4249369999 @default.
- W4287987652 doi "https://doi.org/10.1016/bs.mcb.2022.04.005" @default.
- W4287987652 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35953207" @default.
- W4287987652 hasPublicationYear "2022" @default.
- W4287987652 type Work @default.
- W4287987652 citedByCount "1" @default.
- W4287987652 countsByYear W42879876522023 @default.
- W4287987652 crossrefType "book-chapter" @default.
- W4287987652 hasAuthorship W4287987652A5038401147 @default.
- W4287987652 hasAuthorship W4287987652A5056606171 @default.
- W4287987652 hasAuthorship W4287987652A5059744998 @default.
- W4287987652 hasConcept C109159458 @default.
- W4287987652 hasConcept C112392421 @default.
- W4287987652 hasConcept C157333092 @default.
- W4287987652 hasConcept C201750760 @default.
- W4287987652 hasConcept C203014093 @default.
- W4287987652 hasConcept C2778837598 @default.
- W4287987652 hasConcept C2779282312 @default.
- W4287987652 hasConcept C2780007613 @default.
- W4287987652 hasConcept C2780076729 @default.
- W4287987652 hasConcept C2781057849 @default.
- W4287987652 hasConcept C28328180 @default.
- W4287987652 hasConcept C32465701 @default.
- W4287987652 hasConcept C42362537 @default.
- W4287987652 hasConcept C502942594 @default.
- W4287987652 hasConcept C62478195 @default.
- W4287987652 hasConcept C75217442 @default.
- W4287987652 hasConcept C86554907 @default.
- W4287987652 hasConcept C86803240 @default.
- W4287987652 hasConcept C95444343 @default.
- W4287987652 hasConceptScore W4287987652C109159458 @default.
- W4287987652 hasConceptScore W4287987652C112392421 @default.
- W4287987652 hasConceptScore W4287987652C157333092 @default.