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- W4288045251 abstract "Tissue-resident macrophages (TRMs) perform organ-specific functions that are dependent on factors such as hematopoietic origin, local environment, and biological influences. A diverse range of in vitro culture systems have been developed to decipher TRM functions, including bone marrow-derived macrophages (BMDMs), induced pluripotent stem cell (iPSC)-derived TRMs, or immortalized cell lines. However, despite the usefulness of such systems, there are notable limitations. Attempts to culture primary macrophages often require purification of cells and lack a high cell yield and consistent phenotype. Here, we aimed to address these limitations by establishing an organotypic primary cell culture protocol. We obtained long-term monocultures of macrophages derived from distinct organs without prior purification using specific growth factors and tissue normoxic conditions that largely conserved a TRM-like identity in vitro. Thus, this organotypic system offers an ideal screening platform for primary macrophages from different organs that can be used for a wide range of assays and readouts." @default.
- W4288045251 created "2022-07-27" @default.
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- W4288045251 date "2022-08-01" @default.
- W4288045251 modified "2023-10-13" @default.
- W4288045251 title "An improved organotypic cell culture system to study tissue-resident macrophages ex vivo" @default.
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- W4288045251 doi "https://doi.org/10.1016/j.crmeth.2022.100260" @default.
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