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- W4288045478 endingPage "130216" @default.
- W4288045478 startingPage "130216" @default.
- W4288045478 abstract "Apurinic/apyrimidinic (AP) endonuclease Nfo from Escherichia coli recognises AP sites in DNA and catalyses phosphodiester bond cleavage on the 5' side of AP sites and some damaged or undamaged nucleotides. Here, the mechanism of target nucleotide recognition by Nfo was analysed by pulsed electron-electron double resonance (PELDOR, also known as DEER) spectroscopy and pre-steady-state kinetic analysis with Förster resonance energy transfer detection of DNA conformational changes during DNA binding. The efficiency of endonucleolytic cleavage of a target nucleotide in model DNA substrates was ranked as (2R,3S)-2-(hydroxymethyl)-3-hydroxytetrahydrofuran [F-site] > 5,6-dihydro-2'-deoxyuridine > α-anomer of 2'-deoxyadenosine >2'-deoxyuridine > undamaged DNA. Real-time conformational changes of DNA during interaction with Nfo revealed an increase of distances between duplex ends during the formation of the initial enzyme-substrate complex. The use of rigid-linker spin-labelled DNA duplexes in DEER measurements indicated that double-helix bending and unwinding by the target nucleotide itself is one of the key factors responsible for indiscriminate recognition of a target nucleotide by Nfo. The results for the first time show that AP endonucleases from different structural families utilise a common strategy of damage recognition, which globally may be integrated with the mechanism of searching for specific sites in DNA by other enzymes." @default.
- W4288045478 created "2022-07-27" @default.
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- W4288045478 date "2022-11-01" @default.
- W4288045478 modified "2023-10-18" @default.
- W4288045478 title "The mechanism of damage recognition by apurinic/apyrimidinic endonuclease Nfo from Escherichia coli" @default.
- W4288045478 cites W1499918639 @default.
- W4288045478 cites W1519057123 @default.
- W4288045478 cites W1535534179 @default.
- W4288045478 cites W1614551514 @default.
- W4288045478 cites W1874262838 @default.
- W4288045478 cites W1911855042 @default.
- W4288045478 cites W1965225383 @default.
- W4288045478 cites W1968895302 @default.
- W4288045478 cites W1969030243 @default.
- W4288045478 cites W1969205934 @default.
- W4288045478 cites W1983898731 @default.
- W4288045478 cites W1987044677 @default.
- W4288045478 cites W1992584287 @default.
- W4288045478 cites W1996245511 @default.
- W4288045478 cites W2000774559 @default.
- W4288045478 cites W2002486758 @default.
- W4288045478 cites W2003447156 @default.
- W4288045478 cites W2007759834 @default.
- W4288045478 cites W2016773562 @default.
- W4288045478 cites W2017807035 @default.
- W4288045478 cites W2019024014 @default.
- W4288045478 cites W2021300382 @default.
- W4288045478 cites W2026161770 @default.
- W4288045478 cites W2030242240 @default.
- W4288045478 cites W2033666481 @default.
- W4288045478 cites W2033735656 @default.
- W4288045478 cites W2034678374 @default.
- W4288045478 cites W2035494017 @default.
- W4288045478 cites W2037431380 @default.
- W4288045478 cites W2040825860 @default.
- W4288045478 cites W2050663632 @default.
- W4288045478 cites W2052679982 @default.
- W4288045478 cites W2053960813 @default.
- W4288045478 cites W2055952174 @default.
- W4288045478 cites W2067546483 @default.
- W4288045478 cites W2068668716 @default.
- W4288045478 cites W2073091925 @default.
- W4288045478 cites W2073553571 @default.
- W4288045478 cites W2078793281 @default.
- W4288045478 cites W2080392363 @default.
- W4288045478 cites W2080623420 @default.
- W4288045478 cites W2088814535 @default.
- W4288045478 cites W2088863316 @default.
- W4288045478 cites W2091521381 @default.
- W4288045478 cites W2093895993 @default.
- W4288045478 cites W2100796216 @default.
- W4288045478 cites W2100904609 @default.
- W4288045478 cites W2125038006 @default.
- W4288045478 cites W2130632113 @default.
- W4288045478 cites W2132766092 @default.
- W4288045478 cites W2133016391 @default.
- W4288045478 cites W2133805329 @default.
- W4288045478 cites W2141980923 @default.
- W4288045478 cites W2142984118 @default.
- W4288045478 cites W2148019381 @default.
- W4288045478 cites W2149174597 @default.
- W4288045478 cites W2149922717 @default.
- W4288045478 cites W2154915448 @default.
- W4288045478 cites W2156112169 @default.
- W4288045478 cites W2323274627 @default.
- W4288045478 cites W2415260692 @default.
- W4288045478 cites W2428398873 @default.
- W4288045478 cites W2474486403 @default.
- W4288045478 cites W2507224841 @default.
- W4288045478 cites W2553169723 @default.
- W4288045478 cites W2574615428 @default.
- W4288045478 cites W2613717888 @default.
- W4288045478 cites W2748537268 @default.
- W4288045478 cites W2763572277 @default.
- W4288045478 cites W2794914378 @default.
- W4288045478 cites W2888530628 @default.
- W4288045478 cites W2897150104 @default.
- W4288045478 cites W2946280249 @default.
- W4288045478 cites W2951785591 @default.
- W4288045478 cites W2982272824 @default.
- W4288045478 cites W3021057201 @default.
- W4288045478 cites W3080163430 @default.
- W4288045478 cites W3082841381 @default.
- W4288045478 cites W3096968888 @default.
- W4288045478 cites W3134296933 @default.
- W4288045478 cites W3150902302 @default.
- W4288045478 cites W3204971563 @default.
- W4288045478 cites W3208345469 @default.
- W4288045478 cites W4206288518 @default.