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- W4288064185 abstract "Visualization of single mRNA molecules in fixed cells can be achieved using single molecule fluorescent in situ hybridization (smFISH). This approach enables accurate quantification of mRNA numbers and localization at a single-cell level. To ensure reliable results using smFISH, it is critical to use fluorescent probes that are highly specific to their RNA target. To facilitate probe design, we have created anglerFISH, a user-friendly command-line based pipeline. In this chapter, we present how to perform a smFISH experiment using user-designed and labeled probes." @default.
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- W4288064185 date "2022-01-01" @default.
- W4288064185 modified "2023-10-17" @default.
- W4288064185 title "Design, Labeling, and Application of Probes for RNA smFISH" @default.
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- W4288064185 doi "https://doi.org/10.1007/978-1-0716-2521-7_10" @default.
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