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- W4288077424 abstract "Chryseobacterium indologenes is a non-glucose-fermenting Gram-negative bacillus. This emerging multidrug resistant opportunistic nosocomial pathogen can cause severe infections in neonates and immunocompromised patients. This study aimed to present the first detailed draft genome sequence of a multidrug-resistant C. indologenes strain isolated from the cerebrospinal fluid of an infant hospitalized at the Neonatal Intensive Care Unit of Brazilian Tertiary Hospital. We first analyzed the susceptibility of C. indologenes strain to different antibiotics using the VITEK 2 system. The strain demonstrated an outstanding resistance to all the antibiotic classes tested, including β-lactams, aminoglycosides, glycylcycline, and polymyxin. Next, C. indologenes was whole-genome-sequenced, annotated using Prokka and Rapid Annotation using Subsystems Technology (RAST), and screened for orthologous groups (EggNOG), gene ontology (GO), resistance genes, virulence genes, and mobile genetic elements using different software tools. The draft genome contained one circular chromosome of 4,836,765 bp with 37.32% GC content. The genomic features of the chromosome present numerous genes related to cellular processes that are essential to bacteria. The MDR C. indologenes revealed the presence of genes that corresponded to the resistance phenotypes, including genes to β-lactamases ( bla IND– 13 , bla CIA– 3 , bla TEM– 116 , bla OXA– 209 , bla VEB– 15 ), quinolone ( mcb G), tigecycline ( tet (X6)), and genes encoding efflux pumps which confer resistance to aminoglycosides ( Ran A/ Ran B), and colistin ( Hly D/ Tol C). Amino acid substitutions related to quinolone resistance were observed in GyrA (S83Y) and GyrB (L425I and K473R). A mutation that may play a role in the development of colistin resistance was detected in lpxA (G68D). Chryseobacterium indologenes isolate harbored 19 virulence factors, most of which were involved in infection pathways. We identified 13 Genomic Islands (GIs) and some elements associated with one integrative and conjugative element (ICEs). Other elements linked to mobile genetic elements (MGEs), such as insertion sequence (ISEIsp1), transposon (Tn5393), and integron (In31), were also present in the C. indologenes genome. Although plasmids were not detected, a ColRNAI replicon type and the most resistance genes detected in singletons were identified in unaligned scaffolds. We provided a wide range of information toward the understanding of the genomic diversity of C. indologenes , which can contribute to controlling the evolution and dissemination of this pathogen in healthcare settings." @default.
- W4288077424 created "2022-07-28" @default.
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- W4288077424 date "2022-07-28" @default.
- W4288077424 modified "2023-10-14" @default.
- W4288077424 title "Whole genome sequencing of the multidrug-resistant Chryseobacterium indologenes isolated from a patient in Brazil" @default.
- W4288077424 cites W1484660603 @default.
- W4288077424 cites W1487634705 @default.
- W4288077424 cites W1496543940 @default.
- W4288077424 cites W1504776901 @default.
- W4288077424 cites W1522534339 @default.
- W4288077424 cites W1569874859 @default.
- W4288077424 cites W1582578266 @default.
- W4288077424 cites W1648964476 @default.
- W4288077424 cites W1791897171 @default.
- W4288077424 cites W1851826491 @default.
- W4288077424 cites W1892148740 @default.
- W4288077424 cites W1960965614 @default.
- W4288077424 cites W1964516944 @default.
- W4288077424 cites W1969407777 @default.
- W4288077424 cites W1970010269 @default.
- W4288077424 cites W1970861246 @default.
- W4288077424 cites W1971856087 @default.
- W4288077424 cites W1981229360 @default.
- W4288077424 cites W1983187070 @default.
- W4288077424 cites W1988297980 @default.
- W4288077424 cites W2004548026 @default.
- W4288077424 cites W2007321059 @default.
- W4288077424 cites W2008072020 @default.
- W4288077424 cites W2008738984 @default.
- W4288077424 cites W2009000877 @default.
- W4288077424 cites W2011591489 @default.
- W4288077424 cites W2016510254 @default.
- W4288077424 cites W2017045389 @default.
- W4288077424 cites W2017747041 @default.
- W4288077424 cites W2030259902 @default.
- W4288077424 cites W2030966943 @default.
- W4288077424 cites W2037429250 @default.
- W4288077424 cites W2042614942 @default.
- W4288077424 cites W2042716741 @default.
- W4288077424 cites W2044894409 @default.
- W4288077424 cites W2047983250 @default.
- W4288077424 cites W2048456982 @default.
- W4288077424 cites W2051163727 @default.
- W4288077424 cites W2053594975 @default.
- W4288077424 cites W2060081211 @default.
- W4288077424 cites W2061578584 @default.
- W4288077424 cites W2065337857 @default.
- W4288077424 cites W2066361251 @default.
- W4288077424 cites W2077326186 @default.
- W4288077424 cites W2079891940 @default.
- W4288077424 cites W2086648625 @default.
- W4288077424 cites W2092775842 @default.
- W4288077424 cites W2094095576 @default.
- W4288077424 cites W2096093282 @default.
- W4288077424 cites W2099055899 @default.
- W4288077424 cites W2099059793 @default.
- W4288077424 cites W2100494916 @default.
- W4288077424 cites W2103996413 @default.
- W4288077424 cites W2105838577 @default.
- W4288077424 cites W2107676349 @default.
- W4288077424 cites W2107772251 @default.
- W4288077424 cites W2118688136 @default.
- W4288077424 cites W2120902911 @default.
- W4288077424 cites W2122623487 @default.
- W4288077424 cites W2123395578 @default.
- W4288077424 cites W2127096898 @default.
- W4288077424 cites W2127447560 @default.
- W4288077424 cites W2129359155 @default.
- W4288077424 cites W2129385896 @default.
- W4288077424 cites W2129744420 @default.
- W4288077424 cites W2133676327 @default.
- W4288077424 cites W2139669372 @default.
- W4288077424 cites W2142343447 @default.
- W4288077424 cites W2143007385 @default.
- W4288077424 cites W2143325926 @default.
- W4288077424 cites W2144047666 @default.
- W4288077424 cites W2149452073 @default.
- W4288077424 cites W2149652213 @default.
- W4288077424 cites W2150922581 @default.
- W4288077424 cites W2151183612 @default.
- W4288077424 cites W2156449112 @default.
- W4288077424 cites W2159008215 @default.
- W4288077424 cites W2160378127 @default.
- W4288077424 cites W2160741207 @default.
- W4288077424 cites W2162404814 @default.
- W4288077424 cites W2164154943 @default.
- W4288077424 cites W2165950082 @default.
- W4288077424 cites W2166683258 @default.