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- W4288077867 abstract "Abstract Riboswitches are regulatory sequences composed of an aptamer domain capable of binding a ligand and an expression platform that allows the control of the downstream gene expression based on a conformational change. Current bioinformatic methods for their discovery have various limitations. To circumvent this, we developed an experimental technique to discover new riboswitches called SR-PAGE (Shifted Reverse Polyacrylamide Gel Electrophoresis). A ligand-based regulatory molecule is recognized by exploiting the conformational change of the sequence following binding with the ligand within a native polyacrylamide gel. Known riboswitches were tested with their corresponding ligands to validate our method. SR-PAGE was imbricated within an SELEX to enrich switching RNAs from a TPP riboswitch-based degenerate library to change its binding preference from TPP to thiamine. The SR-PAGE technique allows performing a large screening for riboswitches, search in several organisms and test more than one ligand simultaneously." @default.
- W4288077867 created "2022-07-28" @default.
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- W4288077867 date "2022-07-26" @default.
- W4288077867 modified "2023-09-27" @default.
- W4288077867 title "Shifted Reverse PAGE: a novel approach based on structure switching for the discovery of riboswitches and aptamers" @default.
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- W4288077867 doi "https://doi.org/10.1101/2022.07.26.501614" @default.
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