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- W4288434553 abstract "In situ conjugation of fluorescent molecules to biomolecules such as proteins under spatiotemporal control offers a powerful means for studying biological systems. For that purpose, the o-quinone methide chemistry involving a sequence of the trigger-release-conjugation (TRC) process provides a versatile conjugation method. We have developed a new TRC platform bearing a quaternary ammonium salt for the release process, which can be structurally modified and readily synthesized from commonly used aryl alcohol-type organic fluorophores under environmentally benign conditions. We show that different aryl alcohol fluorophores containing the o-(morpholinium)methyl group for the release process allow efficient fluorophore labeling of proteins under both light- and chemical-triggering conditions. The bioconjugation in cells as well as in tissues was further demonstrated with an o-(morpholinium)methyl analogue containing a triggering group sensitive to reactive oxygen species. The new TRC system thus provides a versatile and unique platform for in situ fluorophore labeling of proteins in biological systems under spatiotemporal control." @default.
- W4288434553 created "2022-07-29" @default.
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- W4288434553 date "2022-07-28" @default.
- W4288434553 modified "2023-09-29" @default.
- W4288434553 title "Fluorophore Labeling of Proteins: a Versatile Trigger–Release–Conjugation Platform Based on the Quinone Methide Chemistry" @default.
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- W4288434553 doi "https://doi.org/10.1021/acs.bioconjchem.2c00297" @default.
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